7ahl - Revision history

OCA at 14:51, 6 March 2024

2024-03-06T14:51:44Z

←Older revision		Revision as of 14:51, 6 March 2024
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		+
	==ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS==		==ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS==
-	<StructureSection load='7ahl' size='340' side='right' caption='[[7ahl]], [[Resolution\|resolution]] 1.89Å' scene=''>	+	<StructureSection load='7ahl' size='340' side='right'caption='[[7ahl]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=7AHL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AHL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [~~http~~://pdbe.org/7ahl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7ahl PDBsum]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.89Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [https://pdbe.org/7ahl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [https://www.ebi.ac.uk/pdbsum/7ahl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ahl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU]] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.	+	[https://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/7ahl_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/7ahl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 17:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7ahl ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7ahl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The structure of the Staphylococcus aureus alpha-hemolysin pore has been determined to 1.9 A resolution. Contained within the mushroom-shaped homo-oligomeric heptamer is a solvent-filled channel, 100 A in length, that runs along the sevenfold axis and ranges from 14 A to 46 A in diameter. The lytic, transmembrane domain comprises the lower half of a 14-strand antiparallel beta barrel, to which each protomer contributes two beta strands, each 65 A long. The interior of the beta barrel is primarily hydrophilic, and the exterior has a hydrophobic belt 28 A wide. The structure proves the heptameric subunit stoichiometry of the alpha-hemolysin oligomer, shows that a glycine-rich and solvent-exposed region of a water-soluble protein can self-assemble to form a transmembrane pore of defined structure, and provides insight into the principles of membrane interaction and transport activity of beta barrel pore-forming toxins.
-
-	Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore.,Song L, Hobaugh MR, Shustak C, Cheley S, Bayley H, Gouaux JE Science. 1996 Dec 13;274(5294):1859-66. PMID:8943190<ref>PMID:8943190</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7ahl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Hemolysin\|Hemolysin]]		*[[Hemolysin\|Hemolysin]]
		+	*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
	*[[Pore forming toxin%2C ÃÂ±-hemolysin\|Pore forming toxin%2C ÃÂ±-hemolysin]]		*[[Pore forming toxin%2C ÃÂ±-hemolysin\|Pore forming toxin%2C ÃÂ±-hemolysin]]
-	*[[Practical Guide to Homology Modeling\|Practical Guide to Homology Modeling]]
-	*[[User:Eric Martz/JSmol Notes\|User:Eric Martz/JSmol Notes]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Bayley, H]]	+	[[Category: Bayley H]]
-	[[Category: Cheley, S]]	+	[[Category: Cheley S]]
-	[[Category: Gouaux~~, J E~~]]	+	[[Category: Gouaux JE]]
-	[[Category: Hobaugh, M]]	+	[[Category: Hobaugh M]]
-	[[Category: Shustak, C]]	+	[[Category: Shustak C]]
-	[[Category: Song, L]]	+	[[Category: Song L]]
-	~~[[Category: Cytolytic protein]]~~	+
-	~~[[Category: Hemolysin]]~~	+
-	~~[[Category: Transmembrane pore~~]]	+

OCA at 01:28, 7 February 2016

2016-02-07T01:28:59Z

←Older revision		Revision as of 01:28, 7 February 2016
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7AHL FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7AHL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [http://www.ebi.ac.uk/pdbsum/7ahl PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [http://pdbe.org/7ahl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [http://www.ebi.ac.uk/pdbsum/7ahl PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7ahl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 7ahl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Hemolysin\|Hemolysin]]		*[[Hemolysin\|Hemolysin]]
	*[[Pore forming toxin%2C ÃÂ±-hemolysin\|Pore forming toxin%2C ÃÂ±-hemolysin]]		*[[Pore forming toxin%2C ÃÂ±-hemolysin\|Pore forming toxin%2C ÃÂ±-hemolysin]]
		+	*[[Practical Guide to Homology Modeling\|Practical Guide to Homology Modeling]]
	*[[User:Eric Martz/JSmol Notes\|User:Eric Martz/JSmol Notes]]		*[[User:Eric Martz/JSmol Notes\|User:Eric Martz/JSmol Notes]]
	== References ==		== References ==

OCA at 14:13, 24 December 2014

2014-12-24T14:13:06Z

←Older revision		Revision as of 14:13, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7AHL FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7AHL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [http://www.ebi.ac.uk/pdbsum/7ahl PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [http://www.ebi.ac.uk/pdbsum/7ahl PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU]] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Bayley, H.]]	+	[[Category: Bayley, H]]
-	[[Category: Cheley, S.]]	+	[[Category: Cheley, S]]
-	[[Category: Gouaux, J E.]]	+	[[Category: Gouaux, J E]]
-	[[Category: Hobaugh, M.]]	+	[[Category: Hobaugh, M]]
-	[[Category: Shustak, C.]]	+	[[Category: Shustak, C]]
-	[[Category: Song, L.]]	+	[[Category: Song, L]]
	[[Category: Cytolytic protein]]		[[Category: Cytolytic protein]]
	[[Category: Hemolysin]]		[[Category: Hemolysin]]
	[[Category: Transmembrane pore]]		[[Category: Transmembrane pore]]

OCA: Protected "7ahl" [edit=sysop:move=sysop]

2014-09-29T20:22:59Z

Protected "7ahl" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:22, 29 September 2014

OCA: New page: ==ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS== == Structural highl...

2014-09-29T20:22:57Z

New page: ==ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS== <StructureSection load='7ahl' size='340' side='right' caption='7ahl, resolution 1.89Å' scene=''> == Structural highl...

New page

==ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS==
<StructureSection load='7ahl' size='340' side='right' caption='[[7ahl]], [[Resolution|resolution]] 1.89Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7AHL FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [http://www.ebi.ac.uk/pdbsum/7ahl PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
<jmolCheckbox>
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/7ahl_consurf.spt"</scriptWhenChecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the Staphylococcus aureus alpha-hemolysin pore has been determined to 1.9 A resolution. Contained within the mushroom-shaped homo-oligomeric heptamer is a solvent-filled channel, 100 A in length, that runs along the sevenfold axis and ranges from 14 A to 46 A in diameter. The lytic, transmembrane domain comprises the lower half of a 14-strand antiparallel beta barrel, to which each protomer contributes two beta strands, each 65 A long. The interior of the beta barrel is primarily hydrophilic, and the exterior has a hydrophobic belt 28 A wide. The structure proves the heptameric subunit stoichiometry of the alpha-hemolysin oligomer, shows that a glycine-rich and solvent-exposed region of a water-soluble protein can self-assemble to form a transmembrane pore of defined structure, and provides insight into the principles of membrane interaction and transport activity of beta barrel pore-forming toxins.

Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore.,Song L, Hobaugh MR, Shustak C, Cheley S, Bayley H, Gouaux JE Science. 1996 Dec 13;274(5294):1859-66. PMID:8943190<ref>PMID:8943190</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>

==See Also==
*[[Hemolysin|Hemolysin]]
*[[Pore forming toxin%2C ÃÂ±-hemolysin|Pore forming toxin%2C ÃÂ±-hemolysin]]
*[[User:Eric Martz/JSmol Notes|User:Eric Martz/JSmol Notes]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Staphylococcus aureus]]
[[Category: Bayley, H.]]
[[Category: Cheley, S.]]
[[Category: Gouaux, J E.]]
[[Category: Hobaugh, M.]]
[[Category: Shustak, C.]]
[[Category: Song, L.]]
[[Category: Cytolytic protein]]
[[Category: Hemolysin]]
[[Category: Transmembrane pore]]