OCA at 12:27, 9 June 2021

2021-06-09T12:27:10Z

←Older revision		Revision as of 12:27, 9 June 2021
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	==Structure of Human Potassium Chloride Transporter KCC3 S45D/T940D/T997D in NaCl (Reference Map)==		==Structure of Human Potassium Chloride Transporter KCC3 S45D/T940D/T997D in NaCl (Reference Map)==
-	<StructureSection load='7ain' size='340' side='right'caption='[[7ain]]' scene=''>	+	<StructureSection load='7ain' size='340' side='right'caption='[[7ain]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ain]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AIN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ain OCA], [https://pdbe.org/7ain PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ain RCSB], [https://www.ebi.ac.uk/pdbsum/7ain PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ain ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC12A6, KCC3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ain OCA], [https://pdbe.org/7ain PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ain RCSB], [https://www.ebi.ac.uk/pdbsum/7ain PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ain ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[https://www.uniprot.org/uniprot/S12A6_HUMAN S12A6_HUMAN]] Corpus callosum agenesis-neuronopathy syndrome. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/S12A6_HUMAN S12A6_HUMAN]] Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Potassium-coupled chloride transporters (KCCs) play crucial roles in regulating cell volume and intracellular chloride concentration. They are characteristically inhibited under isotonic conditions via phospho-regulatory sites located within the cytoplasmic termini. Decreased inhibitory phosphorylation in response to hypotonic cell swelling stimulates transport activity, and dysfunction of this regulatory process has been associated with various human diseases. Here, we present cryo-EM structures of human KCC3b and KCC1, revealing structural determinants for phospho-regulation in both N- and C-termini. We show that phospho-mimetic KCC3b is arrested in an inward-facing state in which intracellular ion access is blocked by extensive contacts with the N-terminus. In another mutant with increased isotonic transport activity, KCC1Delta19, this interdomain interaction is absent, likely due to a unique phospho-regulatory site in the KCC1 N-terminus. Furthermore, we map additional phosphorylation sites as well as a previously unknown ATP/ADP-binding pocket in the large C-terminal domain and show enhanced thermal stabilization of other CCCs by adenine nucleotides. These findings provide fundamentally new insights into the complex regulation of KCCs and may unlock innovative strategies for drug development.
		+
		+	Phospho-regulation, nucleotide binding and ion access control in potassium-chloride cotransporters.,Chi G, Ebenhoch R, Man H, Tang H, Tremblay LE, Reggiano G, Qiu X, Bohstedt T, Liko I, Almeida FG, Garneau AP, Wang D, McKinley G, Moreau CP, Bountra KD, Abrusci P, Mukhopadhyay SMM, Fernandez-Cid A, Slimani S, Lavoie JL, Burgess-Brown NA, Tehan B, DiMaio F, Jazayeri A, Isenring P, Robinson CV, Durr KL EMBO J. 2021 May 25:e107294. doi: 10.15252/embj.2020107294. PMID:34031912<ref>PMID:34031912</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ain" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abrusci P]]	+	[[Category: Abrusci, P]]
-	[[Category: Arrowsmith CH]]	+	[[Category: Arrowsmith, C H]]
-	[[Category: Bohstedt T]]	+	[[Category: Bohstedt, T]]
-	[[Category: Bountra C]]	+	[[Category: Bountra, C]]
-	[[Category: Burgess-Brown NA]]	+	[[Category: Burgess-Brown, N A]]
-	[[Category: Chalk R]]	+	[[Category: Chalk, R]]
-	[[Category: Chi G]]	+	[[Category: Chi, G]]
-	[[Category: DiMaio F]]	+	[[Category: DiMaio, F]]
-	[[Category: Duerr KL]]	+	[[Category: Duerr, K L]]
-	[[Category: Ebenhoch R]]	+	[[Category: Ebenhoch, R]]
-	[[Category: Edwards AM]]	+	[[Category: Edwards, A M]]
-	[[Category: MacLean EM]]	+	[[Category: MacLean, E M]]
-	[[Category: Man H]]	+	[[Category: Man, H]]
-	[[Category: Marsden BD]]	+	[[Category: Marsden, B D]]
-	[[Category: McKinley G]]	+	[[Category: McKinley, G]]
-	[[Category: Moreau C]]	+	[[Category: Moreau, C]]
-	[[Category: Mukhopadhyay ~~SMM~~]]	+	[[Category: Mukhopadhyay, S M.M]]
-	[[Category: Pike ~~ACW~~]]	+	[[Category: Pike, A C.W]]
-	[[Category: Reggiano G]]	+	[[Category: Reggiano, G]]
-	[[Category: Singh NK]]	+	[[Category: Structural genomic]]
-	[[Category: Snee M]]	+	[[Category: Singh, N K]]
-	[[Category: Wang D]]	+	[[Category: Snee, M]]
		+	[[Category: Wang, D]]
		+	[[Category: Ccc]]
		+	[[Category: Homodimer]]
		+	[[Category: Human membrane protein]]
		+	[[Category: Kcc]]
		+	[[Category: Kcc3]]
		+	[[Category: Potassium-chloride coupled transporter]]
		+	[[Category: Sgc]]
		+	[[Category: Transport protein]]
		+	[[Category: Transporter]]

OCA at 14:33, 2 June 2021

2021-06-02T14:33:39Z

←Older revision		Revision as of 14:33, 2 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7ain is ~~ON HOLD until Paper Publication~~	+	==Structure of Human Potassium Chloride Transporter KCC3 S45D/T940D/T997D in NaCl (Reference Map)==
-		+	<StructureSection load='7ain' size='340' side='right'caption='[[7ain]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AIN FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ain OCA], [https://pdbe.org/7ain PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ain RCSB], [https://www.ebi.ac.uk/pdbsum/7ain PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ain ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Abrusci P]]
		+	[[Category: Arrowsmith CH]]
		+	[[Category: Bohstedt T]]
		+	[[Category: Bountra C]]
		+	[[Category: Burgess-Brown NA]]
		+	[[Category: Chalk R]]
		+	[[Category: Chi G]]
		+	[[Category: DiMaio F]]
		+	[[Category: Duerr KL]]
		+	[[Category: Ebenhoch R]]
		+	[[Category: Edwards AM]]
		+	[[Category: MacLean EM]]
		+	[[Category: Man H]]
		+	[[Category: Marsden BD]]
		+	[[Category: McKinley G]]
		+	[[Category: Moreau C]]
		+	[[Category: Mukhopadhyay SMM]]
		+	[[Category: Pike ACW]]
		+	[[Category: Reggiano G]]
		+	[[Category: Singh NK]]
		+	[[Category: Snee M]]
		+	[[Category: Wang D]]

OCA at 18:53, 28 October 2020

2020-10-28T18:53:07Z

←Older revision		Revision as of 18:53, 28 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ain is ON HOLD	+	The entry 7ain is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ain" [edit=sysop:move=sysop]

2020-10-07T04:25:20Z

Protected "7ain" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:25, 7 October 2020

OCA: New page: '''Unreleased structure''' The entry 7ain is ON HOLD Authors: Description: Category: Unreleased Structures

2020-10-07T04:25:19Z

New page: '''Unreleased structure''' The entry 7ain is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ain is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7ain - Revision history

OCA at 12:27, 9 June 2021

OCA at 14:33, 2 June 2021

OCA at 18:53, 28 October 2020

OCA: Protected "7ain" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ain is ON HOLD Authors: Description: Category: Unreleased Structures