7b1s - Revision history

OCA at 12:20, 1 February 2024

2024-02-01T12:20:36Z

←Older revision		Revision as of 12:20, 1 February 2024
Line 1:		Line 1:

-	====	+	==Crystal structure of the ethyl-coenzyme M reductase from Candidatus Ethanoperedens thermophilum at 0.994-A resolution==
-	<StructureSection load='7b1s' size='340' side='right'caption='[[7b1s]]' scene=''>	+	<StructureSection load='7b1s' size='340' side='right'caption='[[7b1s]], [[Resolution\|resolution]] 0.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7b1s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Ethanoperedens_thermophilum Candidatus Ethanoperedens thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B1S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b1s OCA], [https://pdbe.org/7b1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b1s RCSB], [https://www.ebi.ac.uk/pdbsum/7b1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b1s ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.992Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGM:5-METHYL-ARGININE'>AGM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COM:1-THIOETHANESULFONIC+ACID'>COM</scene>, <scene name='pdbligand=GL3:THIOGLYCIN'>GL3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=I2M:3-METHYL-L-ALLOISOLEUCINE'>I2M</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MGN:2-METHYL-GLUTAMINE'>MGN</scene>, <scene name='pdbligand=MHS:N1-METHYLATED+HISTIDINE'>MHS</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene>, <scene name='pdbligand=TP7:COENZYME+B'>TP7</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=USN:Dimethylated-F430+cofactor'>USN</scene>, <scene name='pdbligand=UUT:(2S)-2-{[(2S)-2-{[(2S)-2-hydroxypropyl]oxy}propyl]oxy}propan-1-ol'>UUT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b1s OCA], [https://pdbe.org/7b1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b1s RCSB], [https://www.ebi.ac.uk/pdbsum/7b1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b1s ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A7R9R780_9EURY A0A7R9R780_9EURY]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ethane, the second most abundant hydrocarbon gas in the seafloor, is efficiently oxidized by anaerobic archaea in syntrophy with sulfate-reducing bacteria. Here, we report the 0.99-angstrom-resolution structure of the proposed ethane-activating enzyme and describe the specific traits that distinguish it from methane-generating and -consuming methyl-coenzyme M reductases. The widened catalytic chamber, harboring a dimethylated nickel-containing F(430) cofactor, would adapt the chemistry of methyl-coenzyme M reductases for a two-carbon substrate. A sulfur from methionine replaces the oxygen from a canonical glutamine as the nickel lower-axial ligand, a feature conserved in thermophilic ethanotrophs. Specific loop extensions, a four-helix bundle dilatation, and posttranslational methylations result in the formation of a 33-angstrom-long hydrophobic tunnel, which guides the ethane to the buried active site as confirmed with xenon pressurization experiments.
		+
		+	Crystal structure of a key enzyme for anaerobic ethane activation.,Hahn CJ, Lemaire ON, Kahnt J, Engilberge S, Wegener G, Wagner T Science. 2021 Jul 2;373(6550):118-121. doi: 10.1126/science.abg1765. PMID:34210888<ref>PMID:34210888</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7b1s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candidatus Ethanoperedens thermophilum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Engilberge S]]
		+	[[Category: Lemaire ON]]
		+	[[Category: Wagner T]]

OCA at 09:51, 14 July 2021

2021-07-14T09:51:05Z

←Older revision		Revision as of 09:51, 14 July 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7b1s ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7b1s' size='340' side='right'caption='[[7b1s]]' scene=''>
-	~~Authors~~: ~~Wagner, T~~.~~, Lemaire, O~~.N.~~, Engilberge, S~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal structure of~~ the ~~ethyl-coenzyme M reductase from Candidatus Ethanoperedens thermophilum at 0~~.~~994-A resolution~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b1s OCA], [https://pdbe.org/7b1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b1s RCSB], [https://www.ebi.ac.uk/pdbsum/7b1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b1s ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Wagner~~, T]]	+	__TOC__
-	[[Category: ~~Lemaire, O.N~~]]	+	</StructureSection>
-	[[Category: ~~Engilberge, S~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:11, 31 March 2021

2021-03-31T09:11:54Z

←Older revision		Revision as of 09:11, 31 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7b1s is ON HOLD	+	The entry 7b1s is ON HOLD until Paper Publication

	Authors: Wagner, T., Lemaire, O.N., Engilberge, S.		Authors: Wagner, T., Lemaire, O.N., Engilberge, S.

OCA at 05:19, 24 March 2021

2021-03-24T05:19:52Z

←Older revision		Revision as of 05:19, 24 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7b1s is ON HOLD ~~until sometime in the future~~	+	The entry 7b1s is ON HOLD

	Authors: Wagner, T., Lemaire, O.N., Engilberge, S.		Authors: Wagner, T., Lemaire, O.N., Engilberge, S.

OCA at 04:59, 9 December 2020

2020-12-09T04:59:10Z

←Older revision		Revision as of 04:59, 9 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7b1s is ON HOLD	+	The entry 7b1s is ON HOLD until sometime in the future

	Authors: Wagner, T., Lemaire, O.N., Engilberge, S.		Authors: Wagner, T., Lemaire, O.N., Engilberge, S.

OCA: Protected "7b1s" [edit=sysop:move=sysop]

2020-12-02T05:35:18Z

Protected "7b1s" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:35, 2 December 2020

OCA: New page: '''Unreleased structure''' The entry 7b1s is ON HOLD Authors: Wagner, T., Lemaire, O.N., Engilberge, S. Description: Crystal structure of the ethyl-coenzyme M reductase from Candidatus...

2020-12-02T05:35:16Z

New page: '''Unreleased structure''' The entry 7b1s is ON HOLD Authors: Wagner, T., Lemaire, O.N., Engilberge, S. Description: Crystal structure of the ethyl-coenzyme M reductase from Candidatus...

New page

'''Unreleased structure'''

The entry 7b1s is ON HOLD

Authors: Wagner, T., Lemaire, O.N., Engilberge, S.

Description: Crystal structure of the ethyl-coenzyme M reductase from Candidatus Ethanoperedens thermophilum at 0.994-A resolution
[[Category: Unreleased Structures]]
[[Category: Wagner, T]]
[[Category: Lemaire, O.N]]
[[Category: Engilberge, S]]