7bbr - Revision history

OCA at 06:29, 25 August 2021

2021-08-25T06:29:30Z

←Older revision		Revision as of 06:29, 25 August 2021
Line 1:		Line 1:

	==Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T==		==Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T==
-	<StructureSection load='7bbr' size='340' side='right'caption='[[7bbr]]' scene=''>	+	<StructureSection load='7bbr' size='340' side='right'caption='[[7bbr]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bbr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Advmd Advmd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbr OCA], [https://pdbe.org/7bbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbr RCSB], [https://www.ebi.ac.uk/pdbsum/7bbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbr ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KDG:2-KETO-3-DEOXYGLUCONATE'>KDG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MIM_c39430 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1247726 ADVMD])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbr OCA], [https://pdbe.org/7bbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbr RCSB], [https://www.ebi.ac.uk/pdbsum/7bbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbr ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recently, CxaP, a sugar acid substrate binding protein (SBP) from Advenella mimigardefordensis strain DPN7(T) , was identified as part of a novel sugar uptake strategy. In the present study, the protein was successfully crystallized. Although several SBP structures of tripartite ATP-independent periplasmic transporters have already been solved, this is the first structure of an SBP accepting multiple sugar acid ligands. Protein crystals were obtained with bound d-xylonic acid, d-fuconic acid d-galactonic and d-gluconic acid, respectively. The protein shows the typical structure of an SBP of a tripartite ATP-independent periplasmic transporter consisting of two domains linked by a hinge and spanned by a long alpha-helix. By analysis of the structure, the substrate binding site of the protein was identified. The carboxylic group of the sugar acids interacts with Arg175, whereas the coordination of the hydroxylic groups at positions C2 and C3 is most probably realized by Arg154 and Asn151. Furthermore, it was observed that 2-keto-3-deoxy-d-gluconic acid is bound in protein crystals that were crystallized without the addition of any ligand, indicating that this molecule is prebound to the protein and is displaced by the other ligands if they are available. DATABASE: Structural data of CxaP complexes are available in the worldwide Protein Data Bank (https://www.rcsb.org) under the accession codes 7BBR (2-keto-3-deoxy-d-gluconic acid), 7BCR (d-galactonic acid), 7BCN (d-xylonic acid), 7BCO (d-fuconic acid) and 7BCP (d-gluconic acid).
		+
		+	Crystal structure of the sugar acid-binding protein CxaP from a TRAP transporter in Advenella mimigardefordensis strain DPN7(T).,Schafer L, Meinert-Berning C, Kobus S, Hoppner A, Smits SHJ, Steinbuchel A FEBS J. 2021 Feb 25. doi: 10.1111/febs.15789. PMID:33630388<ref>PMID:33630388</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bbr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Advmd]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hoeppner A]]	+	[[Category: Hoeppner, A]]
-	[[Category: Kobus S]]	+	[[Category: Kobus, S]]
-	[[Category: Meinert C]]	+	[[Category: Meinert, C]]
-	[[Category: Schaefer L]]	+	[[Category: Schaefer, L]]
-	[[Category: Smits SH]]	+	[[Category: Smits, S H]]
-	[[Category: Steinbuechel A]]	+	[[Category: Steinbuechel, A]]
		+	[[Category: Sugar acid substrate binding protein trap transporter]]
		+	[[Category: Sugar binding protein]]

OCA at 06:39, 24 March 2021

2021-03-24T06:39:31Z

←Older revision		Revision as of 06:39, 24 March 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7bbr ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T==
-		+	<StructureSection load='7bbr' size='340' side='right'caption='[[7bbr]]' scene=''>
-	~~Authors~~: ~~Schaefer, L~~., ~~Meinert, C~~., ~~Kobus, S~~., ~~Hoeppner, A~~., ~~Smits, S~~.H., ~~Steinbuechel, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBR FirstGlance]. <br>
-	~~Description: Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbr OCA], [https://pdbe.org/7bbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbr RCSB], [https://www.ebi.ac.uk/pdbsum/7bbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Hoeppner, A]]	+	__TOC__
-	[[Category: ~~Smits,~~ S.H]]	+	</StructureSection>
-	[[Category: ~~Kobus, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Steinbuechel, A~~]]	+	[[Category: Hoeppner A]]
-	[[Category: ~~Schaefer, L~~]]	+	[[Category: Kobus S]]
-	[[Category: ~~Meinert, C~~]]	+	[[Category: Meinert C]]
		+	[[Category: Schaefer L]]
		+	[[Category: Smits SH]]
		+	[[Category: Steinbuechel A]]

OCA at 06:35, 20 January 2021

2021-01-20T06:35:31Z

←Older revision		Revision as of 06:35, 20 January 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bbr is ON HOLD	+	The entry 7bbr is ON HOLD until Paper Publication

	Authors: Schaefer, L., Meinert, C., Kobus, S., Hoeppner, A., Smits, S.H., Steinbuechel, A.		Authors: Schaefer, L., Meinert, C., Kobus, S., Hoeppner, A., Smits, S.H., Steinbuechel, A.
Line 7:		Line 7:
	Description: Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T		Description: Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Hoeppner, A]]
		+	[[Category: Smits, S.H]]
		+	[[Category: Kobus, S]]
	[[Category: Steinbuechel, A]]		[[Category: Steinbuechel, A]]
	[[Category: Schaefer, L]]		[[Category: Schaefer, L]]
	[[Category: Meinert, C]]		[[Category: Meinert, C]]
-	[[Category: Smits, S.H]]
-	[[Category: Kobus, S]]
-	[[Category: Hoeppner, A]]

OCA: Protected "7bbr" [edit=sysop:move=sysop]

2020-12-24T07:51:47Z

Protected "7bbr" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:51, 24 December 2020

OCA: New page: '''Unreleased structure''' The entry 7bbr is ON HOLD Authors: Schaefer, L., Meinert, C., Kobus, S., Hoeppner, A., Smits, S.H., Steinbuechel, A. Description: Crystal structure of the su...

2020-12-24T07:51:45Z

New page: '''Unreleased structure''' The entry 7bbr is ON HOLD Authors: Schaefer, L., Meinert, C., Kobus, S., Hoeppner, A., Smits, S.H., Steinbuechel, A. Description: Crystal structure of the su...

New page

'''Unreleased structure'''

The entry 7bbr is ON HOLD

Authors: Schaefer, L., Meinert, C., Kobus, S., Hoeppner, A., Smits, S.H., Steinbuechel, A.

Description: Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T
[[Category: Unreleased Structures]]
[[Category: Steinbuechel, A]]
[[Category: Schaefer, L]]
[[Category: Meinert, C]]
[[Category: Smits, S.H]]
[[Category: Kobus, S]]
[[Category: Hoeppner, A]]