7bbw - Revision history

OCA at 12:25, 1 February 2024

2024-02-01T12:25:28Z

←Older revision		Revision as of 12:25, 1 February 2024
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	<StructureSection load='7bbw' size='340' side='right'caption='[[7bbw]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='7bbw' size='340' side='right'caption='[[7bbw]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7bbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"diplococcus_gonorrhoeae"_(zopf_1885)_lehmann_and_neumann_1896 "diplococcus~~ gonorrhoeae~~" (zopf 1885) lehmann and neumann 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.25Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">tal, E8M68_10680, F9Z35_1423, TUM15744_11190, TUM15745_13730, TUM15746_13410, TUM15747_14120, TUM15749_15370, TUM15750_11410, TUM15751_15520, TUM15759_11670, TUM15760_12360, TUM15761_09930, TUM15764_14310, TUM15765_13050, TUM15766_06460, TUM15768_14740, TUM15770_09320, TUM15771_08190, TUM15772_10900, TUM15774_13280, TUM15776_12130, TUM15780_14920, TUM15781_07470, TUM15782_07520, TUM15784_12210, TUM15785_13660, TUM15786_07850, TUM15787_13260, TUM15789_13310, TUM15790_01750, TUM15791_13450, TUM15792_03840, TUM15793_14160, TUM15796_12040, TUM15797_11040, TUM15798_01870, WHOO_01512, WHOO_01712 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~485~~ "~~Diplococcus gonorrhoeae~~" ~~(Zopf 1885) Lehmann and Neumann 1896])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbw OCA], [https://pdbe.org/7bbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbw RCSB], [https://www.ebi.ac.uk/pdbsum/7bbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbw OCA], [https://pdbe.org/7bbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbw RCSB], [https://www.ebi.ac.uk/pdbsum/7bbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~A0A1D3FXY0_NEIGO A0A1D3FXY0_NEIGO]~~] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.~~[ARBA:ARBA00003518][HAMAP-Rule:MF_00493]~~	+	[https://www.uniprot.org/uniprot/TAL_NEIG1 TAL_NEIG1] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7bbw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7bbw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transaldolase 3D structures\|Transaldolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Transaldolase~~]]	+	[[Category: Neisseria gonorrhoeae]]
-	[[Category: Pappenheim, F ~~Rabe von~~]]	+	[[Category: Rabe von Pappenheim F]]
-	[[Category: Tittmann, K]]	+	[[Category: Tittmann K]]
-	[[Category: Wensien, M]]	+	[[Category: Wensien M]]
-	~~[[Category: Cross-link]]~~	+
-	~~[[Category: Regulation]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:22, 19 May 2021

2021-05-19T10:22:49Z

←Older revision		Revision as of 10:22, 19 May 2021
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/A0A1D3FXY0_NEIGO A0A1D3FXY0_NEIGO]] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.[ARBA:ARBA00003518][HAMAP-Rule:MF_00493]		[[https://www.uniprot.org/uniprot/A0A1D3FXY0_NEIGO A0A1D3FXY0_NEIGO]] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.[ARBA:ARBA00003518][HAMAP-Rule:MF_00493]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Disulfide bonds between cysteine residues are important post-translational modifications in proteins that have critical roles for protein structure and stability, as redox-active catalytic groups in enzymes or allosteric redox switches that govern protein function(1-4). In addition to forming disulfide bridges, cysteine residues are susceptible to oxidation by reactive oxygen species, and are thus central not only to the scavenging of these but also to cellular signalling and communication in biological as well as pathological contexts(5,6). Oxidized cysteine species are highly reactive and may form covalent conjugates with, for example, tyrosines in the active sites of some redox enzymes(7,8). However, to our knowledge, regulatory switches with covalent crosslinks other than disulfides have not previously been demonstrated. Here we report the discovery of a covalent crosslink between a cysteine and a lysine residue with a NOS bridge that serves as an allosteric redox switch in the transaldolase enzyme of Neisseria gonorrhoeae, the pathogen that causes gonorrhoea. X-ray structure analysis of the protein in the oxidized and reduced state reveals a loaded-spring mechanism that involves a structural relaxation upon redox activation, which is propagated from the allosteric redox switch at the protein surface to the active site in the protein interior. This relaxation leads to a reconfiguration of key catalytic residues and elicits an increase in enzymatic activity of several orders of magnitude. The redox switch is highly conserved in related transaldolases from other members of the Neisseriaceae; for example, it is present in the transaldolase of Neisseria meningitides (a pathogen that is the primary cause of meningitis and septicaemia in children). We surveyed the Protein Data Bank and found that the NOS bridge exists in diverse protein families across all domains of life (including Homo sapiens) and that it is often located at catalytic or regulatory hotspots. Our findings will inform strategies for the design of proteins and peptides, as well as the development of new classes of drugs and antibodies that target the lysine-cysteine redox switch(9,10).
		+
		+	A lysine-cysteine redox switch with an NOS bridge regulates enzyme function.,Wensien M, von Pappenheim FR, Funk LM, Kloskowski P, Curth U, Diederichsen U, Uranga J, Ye J, Fang P, Pan KT, Urlaub H, Mata RA, Sautner V, Tittmann K Nature. 2021 May 5. pii: 10.1038/s41586-021-03513-3. doi:, 10.1038/s41586-021-03513-3. PMID:33953398<ref>PMID:33953398</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bbw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:37, 12 May 2021

2021-05-12T09:37:41Z

←Older revision		Revision as of 09:37, 12 May 2021
Line 1:		Line 1:

	==Neisseria gonorrhoeae transaldolase, variant C38S==		==Neisseria gonorrhoeae transaldolase, variant C38S==
-	<StructureSection load='7bbw' size='340' side='right'caption='[[7bbw]]' scene=''>	+	<StructureSection load='7bbw' size='340' side='right'caption='[[7bbw]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"diplococcus_gonorrhoeae"_(zopf_1885)_lehmann_and_neumann_1896 "diplococcus gonorrhoeae" (zopf 1885) lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbw OCA], [https://pdbe.org/7bbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbw RCSB], [https://www.ebi.ac.uk/pdbsum/7bbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbw ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tal, E8M68_10680, F9Z35_1423, TUM15744_11190, TUM15745_13730, TUM15746_13410, TUM15747_14120, TUM15749_15370, TUM15750_11410, TUM15751_15520, TUM15759_11670, TUM15760_12360, TUM15761_09930, TUM15764_14310, TUM15765_13050, TUM15766_06460, TUM15768_14740, TUM15770_09320, TUM15771_08190, TUM15772_10900, TUM15774_13280, TUM15776_12130, TUM15780_14920, TUM15781_07470, TUM15782_07520, TUM15784_12210, TUM15785_13660, TUM15786_07850, TUM15787_13260, TUM15789_13310, TUM15790_01750, TUM15791_13450, TUM15792_03840, TUM15793_14160, TUM15796_12040, TUM15797_11040, TUM15798_01870, WHOO_01512, WHOO_01712 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=485 "Diplococcus gonorrhoeae" (Zopf 1885) Lehmann and Neumann 1896])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbw OCA], [https://pdbe.org/7bbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbw RCSB], [https://www.ebi.ac.uk/pdbsum/7bbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A1D3FXY0_NEIGO A0A1D3FXY0_NEIGO]] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.[ARBA:ARBA00003518][HAMAP-Rule:MF_00493]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Rabe von~~ Pappenheim F]]	+	[[Category: Transaldolase]]
-	[[Category: Tittmann K]]	+	[[Category: Pappenheim, F Rabe von]]
-	[[Category: Wensien M]]	+	[[Category: Tittmann, K]]
		+	[[Category: Wensien, M]]
		+	[[Category: Cross-link]]
		+	[[Category: Regulation]]
		+	[[Category: Transferase]]

OCA at 06:39, 24 March 2021

2021-03-24T06:39:30Z

←Older revision		Revision as of 06:39, 24 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7bbw ~~is ON HOLD until Paper Publication~~	+	==Neisseria gonorrhoeae transaldolase, variant C38S==
-		+	<StructureSection load='7bbw' size='340' side='right'caption='[[7bbw]]' scene=''>
-	~~Authors~~: ~~Rabe von Pappenheim~~, F., ~~Wensien~~, M., ~~Tittmann~~, K.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBW FirstGlance]. <br>
-	~~Description: Neisseria gonorrhoeae transaldolase, variant C38S~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbw OCA], [https://pdbe.org/7bbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbw RCSB], [https://www.ebi.ac.uk/pdbsum/7bbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbw ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Wensien, M~~]]	+	__TOC__
-	[[Category: Tittmann, K]]	+	</StructureSection>
-	[[Category: ~~Rabe Von Pappenheim, F~~]]	+	[[Category: Large Structures]]
		+	[[Category: Rabe von Pappenheim F]]
		+	[[Category: Tittmann K]]
		+	[[Category: Wensien M]]

OCA: Protected "7bbw" [edit=sysop:move=sysop]

2020-12-30T05:15:07Z

Protected "7bbw" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:15, 30 December 2020

OCA: New page: '''Unreleased structure''' The entry 7bbw is ON HOLD until Paper Publication Authors: Rabe von Pappenheim, F., Wensien, M., Tittmann, K. Description: Neisseria gonorrhoeae transaldolas...

2020-12-30T05:15:06Z

New page: '''Unreleased structure''' The entry 7bbw is ON HOLD until Paper Publication Authors: Rabe von Pappenheim, F., Wensien, M., Tittmann, K. Description: Neisseria gonorrhoeae transaldolas...

New page

'''Unreleased structure'''

The entry 7bbw is ON HOLD until Paper Publication

Authors: Rabe von Pappenheim, F., Wensien, M., Tittmann, K.

Description: Neisseria gonorrhoeae transaldolase, variant C38S
[[Category: Unreleased Structures]]
[[Category: Wensien, M]]
[[Category: Tittmann, K]]
[[Category: Rabe Von Pappenheim, F]]