7bio - Revision history

OCA at 12:29, 1 February 2024

2024-02-01T12:29:06Z

←Older revision		Revision as of 12:29, 1 February 2024
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	<StructureSection load='7bio' size='340' side='right'caption='[[7bio]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='7bio' size='340' side='right'caption='[[7bio]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7bio]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~As_4.1669 As 4.1669~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bio]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_bottropensis Streptomyces bottropensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.795Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rslO4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42235 AS 4.1669])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bio OCA], [https://pdbe.org/7bio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bio RCSB], [https://www.ebi.ac.uk/pdbsum/7bio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bio ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bio OCA], [https://pdbe.org/7bio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bio RCSB], [https://www.ebi.ac.uk/pdbsum/7bio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bio ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/W8QLE0_9ACTN W8QLE0_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1669]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alali, A]]	+	[[Category: Streptomyces bottropensis]]
-	[[Category: Bechthold, A]]	+	[[Category: Alali A]]
-	[[Category: Einsle, O]]	+	[[Category: Bechthold A]]
-	[[Category: Zhang, L]]	+	[[Category: Einsle O]]
-	[[Category: Zuo, C]]	+	[[Category: Zhang L]]
-	~~[[Category: Oxidoreductase]]~~	+	[[Category: Zuo C]]
-	~~[[Category: Rishirilides biosynthesis~~]]	+

OCA at 07:11, 7 April 2021

2021-04-07T07:11:05Z

←Older revision		Revision as of 07:11, 7 April 2021
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	==Crystal structure of monooxygenase RslO4 from Streptomyces bottropensis==		==Crystal structure of monooxygenase RslO4 from Streptomyces bottropensis==
-	<StructureSection load='7bio' size='340' side='right'caption='[[7bio]]' scene=''>	+	<StructureSection load='7bio' size='340' side='right'caption='[[7bio]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7BIO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bio]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1669 As 4.1669]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7bio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bio OCA], [~~http~~://pdbe.org/7bio PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7bio RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7bio PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7bio ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rslO4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42235 AS 4.1669])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bio OCA], [https://pdbe.org/7bio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bio RCSB], [https://www.ebi.ac.uk/pdbsum/7bio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bio ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rishirilides are a group of PKS II secondary metabolites produced by Streptomyces bottropensis Go C4/4. Biosynthetic studies in the past have elucidated early and late steps of rishirilide biosynthesis. This work is aiming to solve the remaining steps in the rishirilide biosynthesis. Inactivation of the cyclase gene rslC3 in Streptomyces bottropensis resulted in an interruption of rishirilide production. Instead, accumulation of the tricyclic aromatic galvaquinones was observed. Similar results were observed after deletion of rslO4. Closer inspection into RslO4 crystal structure in addition to site-directed mutagenesis and molecular dynamic simulations revealed that RslO4 might be responsible for quinone formation on the third ring. The RslO1 three-dimensional structure shows a high similarity to FMN-dependent luciferase-like monooxygenases such as the epoxy-forming MsnO8 which acts with the flavin reductase MsnO3 in mensacarcin biosynthesis in the same strain. The high sequence similarity between RslO2 and MsnO3 suggests that RslO2 provides RslO1 with reduced FMN to form an epoxide that serves as substrate for RslO5.
		+
		+	Biosynthesis of the Tricyclic Aromatic Type II Polyketide Rishirilide: New Potential Third Ring Oxygenation after Three Cyclization Steps.,Alali A, Zhang L, Li J, Zuo C, Wassouf D, Yan X, Schwarzer P, Gunther S, Einsle O, Bechthold A Mol Biotechnol. 2021 Mar 24. pii: 10.1007/s12033-021-00314-x. doi:, 10.1007/s12033-021-00314-x. PMID:33763824<ref>PMID:33763824</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bio" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1669]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alali A]]	+	[[Category: Alali, A]]
-	[[Category: Bechthold A]]	+	[[Category: Bechthold, A]]
-	[[Category: Einsle O]]	+	[[Category: Einsle, O]]
-	[[Category: Zhang L]]	+	[[Category: Zhang, L]]
-	[[Category: Zuo C]]	+	[[Category: Zuo, C]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rishirilides biosynthesis]]

OCA: Protected "7bio" [edit=sysop:move=sysop]

2021-01-20T08:13:25Z

Protected "7bio" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:13, 20 January 2021

OCA: New page: ==Crystal structure of monooxygenase RslO4 from Streptomyces bottropensis== == Structural highlights == <...

2021-01-20T08:13:24Z

New page: ==Crystal structure of monooxygenase RslO4 from Streptomyces bottropensis== <StructureSection load='7bio' size='340' side='right'caption='7bio' scene=''> == Structural highlights == <...

New page

==Crystal structure of monooxygenase RslO4 from Streptomyces bottropensis==
<StructureSection load='7bio' size='340' side='right'caption='[[7bio]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BIO FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bio OCA], [http://pdbe.org/7bio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bio RCSB], [http://www.ebi.ac.uk/pdbsum/7bio PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bio ProSAT]</span></td></tr>
</table>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Alali A]]
[[Category: Bechthold A]]
[[Category: Einsle O]]
[[Category: Zhang L]]
[[Category: Zuo C]]