7bvo - Revision history

OCA at 10:05, 6 September 2023

2023-09-06T10:05:34Z

←Older revision		Revision as of 10:05, 6 September 2023
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	==Crystal structure of lysozyme delivered in alginate==		==Crystal structure of lysozyme delivered in alginate==
-	<StructureSection load='7bvo' size='340' side='right'caption='[[7bvo]]' scene=''>	+	<StructureSection load='7bvo' size='340' side='right'caption='[[7bvo]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7BVO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bvo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BVO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7bvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvo OCA], [~~http~~://pdbe.org/7bvo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7bvo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7bvo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvo OCA], [https://pdbe.org/7bvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bvo RCSB], [https://www.ebi.ac.uk/pdbsum/7bvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serial crystallography (SX) provides an opportunity to observe the molecular dynamics of macromolecular structures at room temperature via pump-probe studies. The delivery of crystals embedded in a viscous medium via an injector or syringe is widely performed in synchrotrons or X-ray free-electron laser facilities with low repetition rates. Various viscous media have been developed; however, there are cases in which the delivery material undesirably interacts chemically or biologically with specific protein samples, or changes the stability of the injection stream, depending on the crystallization solution. Therefore, continued discovery and characterization of new delivery media is necessary for expanding future SX applications. Here, the preparation and characterization of new polysaccharide (wheat starch (WS) and alginate)-based sample delivery media are introduced for SX. Crystals embedded in a WS or alginate injection medium showed a stable injection stream at a flow rate of < 200 nL/min and low-level X-ray background scattering similar to other hydrogels. Using these media, serial millisecond crystallography (SMX) was performed, and the room temperature crystal structures of glucose isomerase and lysozyme were determined at 1.9-2.0 A resolutions. WS and alginate will allow an expanded application of sample delivery media in SX experiments.
		+
		+	Polysaccharide-Based Injection Matrix for Serial Crystallography.,Nam KH Int J Mol Sci. 2020 May 8;21(9). pii: ijms21093332. doi: 10.3390/ijms21093332. PMID:32397185<ref>PMID:32397185</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bvo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Nam KH]]		[[Category: Nam KH]]

OCA at 09:40, 18 November 2020

2020-11-18T09:40:49Z

←Older revision		Revision as of 09:40, 18 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7bvo ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of lysozyme delivered in alginate==
-		+	<StructureSection load='7bvo' size='340' side='right'caption='[[7bvo]]' scene=''>
-	~~Authors~~: ~~Nam~~, K.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BVO FirstGlance]. <br>
-	~~Description: Crystal structure of lysozyme delivered in alginate~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvo OCA], [http://pdbe.org/7bvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bvo RCSB], [http://www.ebi.ac.uk/pdbsum/7bvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvo ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Nam~~, K.H~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Nam KH]]

OCA at 05:24, 3 June 2020

2020-06-03T05:24:15Z

←Older revision		Revision as of 05:24, 3 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bvo is ON HOLD	+	The entry 7bvo is ON HOLD until Paper Publication

	Authors: Nam, K.H.		Authors: Nam, K.H.

OCA: Protected "7bvo" [edit=sysop:move=sysop]

2020-04-22T04:36:28Z

Protected "7bvo" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 22 April 2020

OCA: New page: '''Unreleased structure''' The entry 7bvo is ON HOLD Authors: Nam, K.H. Description: Crystal structure of lysozyme delivered in alginate Category: Unreleased Structures [[Category:...

2020-04-22T04:36:26Z

New page: '''Unreleased structure''' The entry 7bvo is ON HOLD Authors: Nam, K.H. Description: Crystal structure of lysozyme delivered in alginate Category: Unreleased Structures [[Category:...

New page

'''Unreleased structure'''

The entry 7bvo is ON HOLD

Authors: Nam, K.H.

Description: Crystal structure of lysozyme delivered in alginate
[[Category: Unreleased Structures]]
[[Category: Nam, K.H]]