7bx9 - Revision history

OCA at 15:39, 29 November 2023

2023-11-29T15:39:00Z

←Older revision		Revision as of 15:39, 29 November 2023
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	<StructureSection load='7bx9' size='340' side='right'caption='[[7bx9]], [[Resolution\|resolution]] 1.38Å' scene=''>		<StructureSection load='7bx9' size='340' side='right'caption='[[7bx9]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7bx9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BX9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bx9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BX9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.38Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azr ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bx9 OCA], [https://pdbe.org/7bx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bx9 RCSB], [https://www.ebi.ac.uk/pdbsum/7bx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bx9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bx9 OCA], [https://pdbe.org/7bx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bx9 RCSB], [https://www.ebi.ac.uk/pdbsum/7bx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bx9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B3VPZ9_BACAM B3VPZ9_BACAM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Khan, F]]	+	[[Category: Khan F]]
-	[[Category: Suguna, K]]	+	[[Category: Suguna K]]
-	~~[[Category: Azo reductase]]~~	+
-	~~[[Category: Azr]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Fmn]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:01, 14 April 2021

2021-04-14T07:01:24Z

←Older revision		Revision as of 07:01, 14 April 2021
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	==Purification, characterization and X-ray structure of YhdA-type azoreductase from Bacillus velezensis==		==Purification, characterization and X-ray structure of YhdA-type azoreductase from Bacillus velezensis==
-	<StructureSection load='7bx9' size='340' side='right'caption='[[7bx9]]' scene=''>	+	<StructureSection load='7bx9' size='340' side='right'caption='[[7bx9]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BX9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7BX9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bx9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BX9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7bx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bx9 OCA], [~~http~~://pdbe.org/7bx9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7bx9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7bx9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7bx9 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azr ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bx9 OCA], [https://pdbe.org/7bx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bx9 RCSB], [https://www.ebi.ac.uk/pdbsum/7bx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bx9 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Azoreductases are being extensively investigated for their ability to initiate degradation of recalcitrant azo dyes through reduction of azo bonds. There is great interest in studying their diversity, structure and function to facilitate better understanding and effective application. Current study reports azoreductase enzyme from Bacillus velezensis, which showed 69.5% identity to the Bacillus subtilis azoreductase YhdA. The enzyme was homotetrameric and molecular weight of each subunit was 20 kDa. It decolourized azo dyes with different structures. The Vmax for decolourization of congo red, methyl orange and methyl red was 14.7, 28.6 and 77.9 nmol/min/mg, respectively. The enzyme contained FMN as cofactor and used NADPH as the favoured co-substrate. It was oxygen-insensitive, but the presence of reducing agents enhanced its activity, which is a new finding. The azoreductase expression in B. velezensis was found to be unaffected by addition of azo dyes, though azo dyes are known to induce azoreductase expression in few organisms. The enzyme was thermostable with melting temperature of 89.5 degrees C and functioned in wide temperature and pH range. Further, the enzyme was crystallized and its structure was solved. The structural basis of its functional attributes is discussed. In our knowledge, this is the first report on characterization of azoreductase enzyme from B. velezensis. This article is protected by copyright. All rights reserved.
		+
		+	Purification, characterization and crystal structure of YhdA-type azoreductase from Bacillus velezensis.,Bafana A, Khan F, Suguna K Proteins. 2020 Dec 1. doi: 10.1002/prot.26032. PMID:33289153<ref>PMID:33289153</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bx9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Khan F]]	+	[[Category: Khan, F]]
-	[[Category: Suguna K]]	+	[[Category: Suguna, K]]
		+	[[Category: Azo reductase]]
		+	[[Category: Azr]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Fmn]]
		+	[[Category: Oxidoreductase]]

OCA at 10:38, 24 December 2020

2020-12-24T10:38:24Z

←Older revision		Revision as of 10:38, 24 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7bx9 ~~is ON HOLD until Paper Publication~~	+	==Purification, characterization and X-ray structure of YhdA-type azoreductase from Bacillus velezensis==
-		+	<StructureSection load='7bx9' size='340' side='right'caption='[[7bx9]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BX9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BX9 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bx9 OCA], [http://pdbe.org/7bx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bx9 RCSB], [http://www.ebi.ac.uk/pdbsum/7bx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bx9 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Khan F]]
		+	[[Category: Suguna K]]

OCA: Protected "7bx9" [edit=sysop:move=sysop]

2020-04-29T05:35:37Z

Protected "7bx9" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:35, 29 April 2020

OCA: New page: '''Unreleased structure''' The entry 7bx9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2020-04-29T05:35:35Z

New page: '''Unreleased structure''' The entry 7bx9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bx9 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]