7c5x - Revision history

OCA at 15:57, 29 November 2023

2023-11-29T15:57:23Z

←Older revision		Revision as of 15:57, 29 November 2023
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	<StructureSection load='7c5x' size='340' side='right'caption='[[7c5x]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='7c5x' size='340' side='right'caption='[[7c5x]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7c5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bigna Bigna~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C5X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7c5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bigelowiella_natans Bigelowiella natans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C5X FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c5x OCA], [https://pdbe.org/7c5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c5x RCSB], [https://www.ebi.ac.uk/pdbsum/7c5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c5x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c5x OCA], [https://pdbe.org/7c5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c5x RCSB], [https://www.ebi.ac.uk/pdbsum/7c5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c5x ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bigna]]~~	+	[[Category: Bigelowiella natans]]
-	~~[[Category: Carbonate dehydratase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Senda, M]]	+	[[Category: Senda M]]
-	[[Category: Senda, T]]	+	[[Category: Senda T]]
-	~~[[Category: Bicarbonate complex]]~~	+
-	~~[[Category: Carbonic anhydrase]]~~	+
-	~~[[Category: Lyase~~]]	+

OCA at 06:30, 25 August 2021

2021-08-25T06:30:02Z

←Older revision		Revision as of 06:30, 25 August 2021
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	==Crystal structure of the iota-carbonic anhydrase from eukaryotic microalga complexed with bicarbonate==		==Crystal structure of the iota-carbonic anhydrase from eukaryotic microalga complexed with bicarbonate==
-	<StructureSection load='7c5x' size='340' side='right'caption='[[7c5x]]' scene=''>	+	<StructureSection load='7c5x' size='340' side='right'caption='[[7c5x]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C5X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7c5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bigna Bigna]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C5X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c5x OCA], [https://pdbe.org/7c5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c5x RCSB], [https://www.ebi.ac.uk/pdbsum/7c5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c5x ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c5x OCA], [https://pdbe.org/7c5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c5x RCSB], [https://www.ebi.ac.uk/pdbsum/7c5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c5x ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Carbonic anhydrases (CAs) are universal metalloenzymes that catalyze the reversible conversion of carbon dioxide (CO2) and bicarbonate (HCO3(-)). They are involved in various biological processes, including pH control, respiration, and photosynthesis. To date, eight evolutionarily unrelated classes of CA families (alpha, beta, gamma, delta, zeta, eta, theta, and iota) have been identified. All are characterized by an active site accommodating the binding of a metal cofactor, which is assumed to play a central role in catalysis. This feature is thought to be the result of convergent evolution. RESULTS: Here, we report that a previously uncharacterized protein group, named "COG4337," constitutes metal-independent CAs from the newly discovered iota-class. Genes coding for COG4337 proteins are found in various bacteria and photosynthetic eukaryotic algae. Biochemical assays demonstrated that recombinant COG4337 proteins from a cyanobacterium (Anabaena sp. PCC7120) and a chlorarachniophyte alga (Bigelowiella natans) accelerated CO2 hydration. Unexpectedly, these proteins exhibited their activity under metal-free conditions. Based on X-ray crystallography and point mutation analysis, we identified a metal-free active site within the cone-shaped alpha+beta barrel structure. Furthermore, subcellular localization experiments revealed that COG4337 proteins are targeted into plastids and mitochondria of B. natans, implicating their involvement in CO2 metabolism in these organelles. CONCLUSIONS: COG4337 proteins shared a short sequence motif and overall structure with iota-class CAs, whereas they were characterized by metal independence, unlike any known CAs. Therefore, COG4337 proteins could be treated as a variant type of iota-class CAs. Our findings suggested that this novel type of iota-CAs can function even in metal-poor environments (e.g., the open ocean) without competition with other metalloproteins for trace metals. Considering the widespread prevalence of iota-CAs across microalgae, this class of CAs may play a role in the global carbon cycle.
		+
		+	Characterization of a novel type of carbonic anhydrase that acts without metal cofactors.,Hirakawa Y, Senda M, Fukuda K, Yu HY, Ishida M, Taira M, Kinbara K, Senda T BMC Biol. 2021 May 18;19(1):105. doi: 10.1186/s12915-021-01039-8. PMID:34006275<ref>PMID:34006275</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7c5x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Carbonic anhydrase 3D structures\|Carbonic anhydrase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bigna]]
		+	[[Category: Carbonate dehydratase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Senda M]]	+	[[Category: Senda, M]]
-	[[Category: Senda T]]	+	[[Category: Senda, T]]
		+	[[Category: Bicarbonate complex]]
		+	[[Category: Carbonic anhydrase]]
		+	[[Category: Lyase]]

OCA at 05:20, 28 April 2021

2021-04-28T05:20:09Z

←Older revision		Revision as of 05:20, 28 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7c5x ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the iota-carbonic anhydrase from eukaryotic microalga complexed with bicarbonate==
-		+	<StructureSection load='7c5x' size='340' side='right'caption='[[7c5x]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C5X FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c5x OCA], [https://pdbe.org/7c5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c5x RCSB], [https://www.ebi.ac.uk/pdbsum/7c5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c5x ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Senda M]]
		+	[[Category: Senda T]]

OCA: Protected "7c5x" [edit=sysop:move=sysop]

2020-05-27T05:18:20Z

Protected "7c5x" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 27 May 2020

OCA: New page: '''Unreleased structure''' The entry 7c5x is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2020-05-27T05:18:18Z

New page: '''Unreleased structure''' The entry 7c5x is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7c5x is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]