OCA at 15:59, 29 November 2023

2023-11-29T15:59:00Z

←Older revision		Revision as of 15:59, 29 November 2023
Line 1:		Line 1:

-	====	+	==Crystal Structure of 14-3-3 epsilon with 9J10 peptide==
-	<StructureSection load='7c8e' size='340' side='right'caption='[[7c8e]]' scene=''>	+	<StructureSection load='7c8e' size='340' side='right'caption='[[7c8e]], [[Resolution\|resolution]] 3.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7c8e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Streptomyces_avermitilis_MA-4680_=_NBRC_14893 Streptomyces avermitilis MA-4680 = NBRC 14893]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C8E FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8e OCA], [https://pdbe.org/7c8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8e RCSB], [https://www.ebi.ac.uk/pdbsum/7c8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8e ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.16Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8e OCA], [https://pdbe.org/7c8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8e RCSB], [https://www.ebi.ac.uk/pdbsum/7c8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8e ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/1433E_HUMAN 1433E_HUMAN] Distal 17p13.3 microdeletion syndrome;17p13.3 microduplication syndrome;Miller-Dieker syndrome.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/1433E_HUMAN 1433E_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Genetic screening technologies to identify and validate macromolecular interactions (MMIs) essential for complex pathways remain an important unmet need for systems biology and therapeutics development. Here, we use a library of peptides from diverse prokaryal genomes to screen MMIs promoting the nuclear relocalization of Forkhead Box O3 (FOXO3a), a tumor suppressor more frequently inactivated by post-translational modification than mutation. A hit peptide engages the 14-3-3 family of signal regulators through a phosphorylation-dependent interaction, modulates FOXO3a-mediated transcription, and suppresses cancer cell growth. In a crystal structure, the hit peptide occupies the phosphopeptide-binding groove of 14-3-3epsilon in a conformation distinct from its natural peptide substrates. A biophysical screen identifies drug-like small molecules that displace the hit peptide from 14-3-3epsilon, providing starting points for structure-guided development. Our findings exemplify "protein interference," an approach using evolutionarily diverse, natural peptides to rapidly identify, validate, and develop chemical probes against MMIs essential for complex cellular phenotypes.
		+
		+	Target identification for small-molecule discovery in the FOXO3a tumor-suppressor pathway using a biodiverse peptide library.,Emery A, Hardwick BS, Crooks AT, Milech N, Watt PM, Mithra C, Kumar V, Giridharan S, Sadasivam G, Mathivanan S, Sudhakar S, Bairy S, Bharatham K, Hurakadli MA, Prasad TK, Kamariah N, Muellner M, Coelho M, Torrance CJ, McKenzie GJ, Venkitaraman AR Cell Chem Biol. 2021 Nov 18;28(11):1602-1615.e9. doi: , 10.1016/j.chembiol.2021.05.009. Epub 2021 Jun 9. PMID:34111400<ref>PMID:34111400</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7c8e" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Streptomyces avermitilis MA-4680 = NBRC 14893]]
		+	[[Category: Bairy S]]
		+	[[Category: Kamariah N]]
		+	[[Category: Mathivanan S]]
		+	[[Category: Sudhakar S]]
		+	[[Category: Venkitaraman A]]

OCA at 14:34, 2 June 2021

2021-06-02T14:34:25Z

←Older revision		Revision as of 14:34, 2 June 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7c8e ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7c8e' size='340' side='right'caption='[[7c8e]]' scene=''>
-	~~Authors~~: ~~Mathivanan, S~~.~~, Sudhakar, S~~.~~, Bairy, S~~.~~, Kamariah, N~~.~~, Venkitaraman, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure of 14-3-3 epsilon with 9J10 peptide~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8e OCA], [https://pdbe.org/7c8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8e RCSB], [https://www.ebi.ac.uk/pdbsum/7c8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8e ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Bairy, S~~]]	+	__TOC__
-	[~~[Category~~: ~~Mathivanan~~, S]]	+	</StructureSection>
-	[~~[Category~~: ~~Venkitaraman~~, A]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kamariah, N~~]]	+	[[Category: Z-disk]]
-	[[Category: ~~Sudhakar, S~~]]	+

OCA: Protected "7c8e" [edit=sysop:move=sysop]

2020-06-10T05:17:53Z

Protected "7c8e" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 7c8e is ON HOLD until Paper Publication Authors: Mathivanan, S., Sudhakar, S., Bairy, S., Kamariah, N., Venkitaraman, A. Description: Crystal Struc...

2020-06-10T05:17:52Z

New page: '''Unreleased structure''' The entry 7c8e is ON HOLD until Paper Publication Authors: Mathivanan, S., Sudhakar, S., Bairy, S., Kamariah, N., Venkitaraman, A. Description: Crystal Struc...

New page

'''Unreleased structure'''

The entry 7c8e is ON HOLD until Paper Publication

Authors: Mathivanan, S., Sudhakar, S., Bairy, S., Kamariah, N., Venkitaraman, A.

Description: Crystal Structure of 14-3-3 epsilon with 9J10 peptide
[[Category: Unreleased Structures]]
[[Category: Bairy, S]]
[[Category: Mathivanan, S]]
[[Category: Venkitaraman, A]]
[[Category: Kamariah, N]]
[[Category: Sudhakar, S]]

7c8e - Revision history

OCA at 15:59, 29 November 2023

OCA at 14:34, 2 June 2021

OCA: Protected "7c8e" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7c8e is ON HOLD until Paper Publication Authors: Mathivanan, S., Sudhakar, S., Bairy, S., Kamariah, N., Venkitaraman, A. Description: Crystal Struc...