7cbc - Revision history

OCA at 06:59, 17 March 2021

2021-03-17T06:59:22Z

←Older revision		Revision as of 06:59, 17 March 2021
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	==Crystal structure of a de novo designed switch protein caging a hemagglutinin binder==		==Crystal structure of a de novo designed switch protein caging a hemagglutinin binder==
-	<StructureSection load='7cbc' size='340' side='right'caption='[[7cbc]]' scene=''>	+	<StructureSection load='7cbc' size='340' side='right'caption='[[7cbc]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7CBC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cbc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CBC FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7cbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbc OCA], [~~http~~://pdbe.org/7cbc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7cbc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7cbc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbc ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbc OCA], [https://pdbe.org/7cbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cbc RCSB], [https://www.ebi.ac.uk/pdbsum/7cbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbc ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Naturally occurring protein switches have been repurposed for the development of biosensors and reporters for cellular and clinical applications(1). However, the number of such switches is limited, and reengineering them is challenging. Here we show that a general class of protein-based biosensors can be created by inverting the flow of information through de novo designed protein switches in which the binding of a peptide key triggers biological outputs of interest(2). The designed sensors are modular molecular devices with a closed dark state and an open luminescent state; analyte binding drives the switch from the closed to the open state. Because the sensor is based on the thermodynamic coupling of analyte binding to sensor activation, only one target binding domain is required, which simplifies sensor design and allows direct readout in solution. We create biosensors that can sensitively detect the anti-apoptosis protein BCL-2, the IgG1 Fc domain, the HER2 receptor, and Botulinum neurotoxin B, as well as biosensors for cardiac troponin I and an anti-hepatitis B virus antibody with the high sensitivity required to detect these molecules clinically. Given the need for diagnostic tools to track the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)(3), we used the approach to design sensors for the SARS-CoV-2 spike protein and antibodies against the membrane and nucleocapsid proteins. The former, which incorporates a de novo designed spike receptor binding domain (RBD) binder(4), has a limit of detection of 15 pM and a luminescence signal 50-fold higher than the background level. The modularity and sensitivity of the platform should enable the rapid construction of sensors for a wide range of analytes, and highlights the power of de novo protein design to create multi-state protein systems with new and useful functions.
		+
		+	De novo design of modular and tunable protein biosensors.,Quijano-Rubio A, Yeh HW, Park J, Lee H, Langan RA, Boyken SE, Lajoie MJ, Cao L, Chow CM, Miranda MC, Wi J, Hong HJ, Stewart L, Oh BH, Baker D Nature. 2021 Jan 27. pii: 10.1038/s41586-021-03258-z. doi:, 10.1038/s41586-021-03258-z. PMID:33503651<ref>PMID:33503651</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cbc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baker D]]	+	[[Category: Synthetic construct sequences]]
-	[[Category: Lee H]]	+	[[Category: Baker, D]]
-	[[Category: Oh B-H]]	+	[[Category: Lee, H]]
		+	[[Category: Oh, B H]]
		+	[[Category: Artificial biosensor]]
		+	[[Category: De novo protein]]
		+	[[Category: Six-helical bundle]]

OCA at 10:38, 24 December 2020

2020-12-24T10:38:36Z

←Older revision		Revision as of 10:38, 24 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7cbc ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of a de novo designed switch protein caging a hemagglutinin binder==
-		+	<StructureSection load='7cbc' size='340' side='right'caption='[[7cbc]]' scene=''>
-	~~Authors~~: ~~Lee~~, H., Oh, B.-H., ~~Baker~~, D.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7CBC FirstGlance]. <br>
-	~~Description: Crystal structure of a de novo designed switch protein caging a hemagglutinin binder~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7cbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbc OCA], [http://pdbe.org/7cbc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7cbc RCSB], [http://www.ebi.ac.uk/pdbsum/7cbc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbc ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Baker, D]]	+	__TOC__
-	[[Category: Lee, H]]	+	</StructureSection>
-	[[Category: Oh, B.-H]]	+	[[Category: Large Structures]]
		+	[[Category: Baker D]]
		+	[[Category: Lee H]]
		+	[[Category: Oh B-H]]

OCA at 18:51, 24 June 2020

2020-06-24T18:51:06Z

←Older revision		Revision as of 18:51, 24 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cbc is ON HOLD	+	The entry 7cbc is ON HOLD until Paper Publication

	Authors: Lee, H., Oh, B.-H., Baker, D.		Authors: Lee, H., Oh, B.-H., Baker, D.

OCA: Protected "7cbc" [edit=sysop:move=sysop]

2020-06-17T09:04:21Z

Protected "7cbc" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:04, 17 June 2020

OCA: New page: '''Unreleased structure''' The entry 7cbc is ON HOLD Authors: Lee, H., Oh, B.-H., Baker, D. Description: Crystal structure of a de novo designed switch protein caging a hemagglutinin b...

2020-06-17T09:04:19Z

New page: '''Unreleased structure''' The entry 7cbc is ON HOLD Authors: Lee, H., Oh, B.-H., Baker, D. Description: Crystal structure of a de novo designed switch protein caging a hemagglutinin b...

New page

'''Unreleased structure'''

The entry 7cbc is ON HOLD

Authors: Lee, H., Oh, B.-H., Baker, D.

Description: Crystal structure of a de novo designed switch protein caging a hemagglutinin binder
[[Category: Unreleased Structures]]
[[Category: Baker, D]]
[[Category: Lee, H]]
[[Category: Oh, B.-H]]