7cpr - Revision history

OCA at 16:13, 29 November 2023

2023-11-29T16:13:31Z

←Older revision		Revision as of 16:13, 29 November 2023
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	==glutamine synthetase from Drosophila==		==glutamine synthetase from Drosophila==
-	<StructureSection load='7cpr' size='340' side='right'caption='[[7cpr]]' scene=''>	+	<StructureSection load='7cpr' size='340' side='right'caption='[[7cpr]], [[Resolution\|resolution]] 2.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CPR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cpr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CPR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cpr OCA], [https://pdbe.org/7cpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cpr RCSB], [https://www.ebi.ac.uk/pdbsum/7cpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cpr ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.12Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cpr OCA], [https://pdbe.org/7cpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cpr RCSB], [https://www.ebi.ac.uk/pdbsum/7cpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cpr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GLNA2_DROME GLNA2_DROME]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutamine synthetase (GS) catalyzes the condensation of ammonia and glutamate, along with ATP, to form glutamine. Despite extensive studies on GSs from eukaryotes and prokaryotes, the roles of the N-terminus and other structural features in catalysis remain unclear. Here we report the decameric structure of Drosophila melanogaster GS 2 (DmGS2). The N-terminal short helices, alpha1 and alpha2, constitute a meander region, and form hydrogen bonds with residues 3-5 in the N-terminal loop, which are not present in the GSs of other species. Deletion of alpha1 or alpha1-alpha2 inactivates DmGS2. Notably, the Arg4 in each monomer of one pentamer forms hydrogen bonds with Glu7, and Asp8 in the adjacent monomer of the other pentamer. Replacement of Arg4 with Asp (R4D) abolishes activity. Analytical ultracentrifugation revealed that Arg4 is crucial for oligomerization. Circular dichroism spectra revealed that R4D may alter the secondary structure. We mutated key residues to identify the substrate-binding site. As Glu140 binds glutamate and Glu311 binds ammonia, mutants E140A and E311A have little activity. Conversely, mutant P214A (P contributes to ATP binding) has higher activity than wild-type DmGS2. These findings expand the understanding of the structural and functional features of the N-terminal meander region of DmGS2 and the residues important for catalytic efficiency.
		+
		+	Structural Insight into the Contributions of the N-Terminus and Key Active-Site Residues to the Catalytic Efficiency of Glutamine Synthetase 2.,Chen WT, Yang HY, Lin CY, Lee YZ, Ma SC, Chen WC, Yin HS Biomolecules. 2020 Dec 14;10(12). pii: biom10121671. doi: 10.3390/biom10121671. PMID:33327463<ref>PMID:33327463</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cpr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutamine synthetase 3D structures\|Glutamine synthetase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chen WT]]		[[Category: Chen WT]]
	[[Category: Yin HS]]		[[Category: Yin HS]]

OCA at 06:05, 11 August 2021

2021-08-11T06:05:52Z

←Older revision		Revision as of 06:05, 11 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7cpr ~~is ON HOLD until Aug 07 2022~~	+	==glutamine synthetase from Drosophila==
-		+	<StructureSection load='7cpr' size='340' side='right'caption='[[7cpr]]' scene=''>
-	~~Authors~~: ~~Yin~~, H.S., ~~Chen~~, W.T.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CPR FirstGlance]. <br>
-	~~Description: glutamine synthetase from Drosophila~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cpr OCA], [https://pdbe.org/7cpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cpr RCSB], [https://www.ebi.ac.uk/pdbsum/7cpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cpr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Yin, H.S~~]]	+	__TOC__
-	[[Category: ~~Chen, W.T~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chen WT]]
		+	[[Category: Yin HS]]

OCA at 08:34, 19 May 2021

2021-05-19T08:34:09Z

←Older revision		Revision as of 08:34, 19 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cpr is ON HOLD	+	The entry 7cpr is ON HOLD until Aug 07 2022

	Authors: Yin, H.S., Chen, W.T.		Authors: Yin, H.S., Chen, W.T.

OCA: Protected "7cpr" [edit=sysop:move=sysop]

2020-08-12T08:26:44Z

Protected "7cpr" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:26, 12 August 2020

OCA: New page: '''Unreleased structure''' The entry 7cpr is ON HOLD Authors: Yin, H.S., Chen, W.T. Description: glutamine synthetase from Drosophila Category: Unreleased Structures [[Category: Yi...

2020-08-12T08:26:42Z

New page: '''Unreleased structure''' The entry 7cpr is ON HOLD Authors: Yin, H.S., Chen, W.T. Description: glutamine synthetase from Drosophila Category: Unreleased Structures [[Category: Yi...

New page

'''Unreleased structure'''

The entry 7cpr is ON HOLD

Authors: Yin, H.S., Chen, W.T.

Description: glutamine synthetase from Drosophila
[[Category: Unreleased Structures]]
[[Category: Yin, H.S]]
[[Category: Chen, W.T]]