OCA at 16:15, 29 November 2023

2023-11-29T16:15:18Z

←Older revision		Revision as of 16:15, 29 November 2023
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	==AtPrR1 with NADP+ and (-)pinoresinol==		==AtPrR1 with NADP+ and (-)pinoresinol==
-	<StructureSection load='7csc' size='340' side='right'caption='[[7csc]]' scene=''>	+	<StructureSection load='7csc' size='340' side='right'caption='[[7csc]], [[Resolution\|resolution]] 2.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CSC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7csc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CSC FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7csc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7csc OCA], [https://pdbe.org/7csc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7csc RCSB], [https://www.ebi.ac.uk/pdbsum/7csc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7csc ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5151186Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GEF:4-[(3R,3aS,6R,6aS)-6-(3-methoxy-4-oxidanyl-phenyl)-1,3,3a,4,6,6a-hexahydrofuro[3,4-c]furan-3-yl]-2-methoxy-phenol'>GEF</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7csc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7csc OCA], [https://pdbe.org/7csc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7csc RCSB], [https://www.ebi.ac.uk/pdbsum/7csc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7csc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PILR1_ARATH PILR1_ARATH] Reductase involved in lignan biosynthesis (PubMed:18347017, PubMed:25107662). Involved in secondary cell wall biosynthesis in fiber cells (PubMed:25107662). Unlike conventional pinoresinol reductases that can reduce both pinoresinol and lariciresinol, PRR1 shows a strict substrate preference toward pinoresinol (PubMed:18347017). Active on both (+) and (-)-pinoresinol (PubMed:18347017). Abstracts the 4R-hydride from the NADPH cofactor during catalysis (PubMed:18347017).<ref>PMID:18347017</ref> <ref>PMID:25107662</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pinoresinol-lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis after the initial dimerization of two monolignols, and this represents the entry point for the synthesis of 8-8' lignans and contributes greatly to their structural diversity. Of particular interest has been the determination of how differing substrate specificities are achieved with these enzymes. Here, we present crystal structures of IiPLR1 from Isatis indigotica and pinoresinol reductases (PrRs) AtPrR1 and AtPrR2 from Arabidopsis thaliana, in the apo, substrate-bound and product-bound states. Each structure contains a head-to-tail homodimer, and the catalytic pocket comprises structural elements from both monomers. beta4 loop covers the top of the pocket, and residue 98 from the loop governs catalytic specificity. The substrate specificities of IiPLR1 and AtPrR2 can be switched via structure-guided mutagenesis. Our study provides insight into the molecular mechanism underlying the substrate specificity of PLRs/PrRs and suggests an efficient strategy for the large-scale commercial production of the pharmaceutically valuable compound lariciresinol.
		+
		+	Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases.,Xiao Y, Shao K, Zhou J, Wang L, Ma X, Wu D, Yang Y, Chen J, Feng J, Qiu S, Lv Z, Zhang L, Zhang P, Chen W Nat Commun. 2021 May 14;12(1):2828. doi: 10.1038/s41467-021-23095-y. PMID:33990581<ref>PMID:33990581</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7csc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Shao K]]		[[Category: Shao K]]
	[[Category: Zhang P]]		[[Category: Zhang P]]

OCA at 12:12, 9 June 2021

2021-06-09T12:12:49Z

←Older revision		Revision as of 12:12, 9 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7csc ~~is ON HOLD~~	+	==AtPrR1 with NADP+ and (-)pinoresinol==
-		+	<StructureSection load='7csc' size='340' side='right'caption='[[7csc]]' scene=''>
-	~~Authors~~: ~~Shao~~, K., ~~Zhang~~, P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CSC FirstGlance]. <br>
-	~~Description~~: ~~AtPrR1 with NADP+ and (~~-~~)pinoresinol~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7csc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7csc OCA], [https://pdbe.org/7csc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7csc RCSB], [https://www.ebi.ac.uk/pdbsum/7csc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7csc ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Shao, K]]	+	__TOC__
-	[[Category: Zhang, P]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Shao K]]
		+	[[Category: Zhang P]]

OCA: Protected "7csc" [edit=sysop:move=sysop]

2020-08-26T09:24:46Z

Protected "7csc" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:24, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7csc is ON HOLD Authors: Shao, K., Zhang, P. Description: AtPrR1 with NADP+ and (-)pinoresinol Category: Unreleased Structures [[Category: Shao...

2020-08-26T09:24:45Z

New page: '''Unreleased structure''' The entry 7csc is ON HOLD Authors: Shao, K., Zhang, P. Description: AtPrR1 with NADP+ and (-)pinoresinol Category: Unreleased Structures [[Category: Shao...

New page

'''Unreleased structure'''

The entry 7csc is ON HOLD

Authors: Shao, K., Zhang, P.

Description: AtPrR1 with NADP+ and (-)pinoresinol
[[Category: Unreleased Structures]]
[[Category: Shao, K]]
[[Category: Zhang, P]]

7csc - Revision history

OCA at 16:15, 29 November 2023

OCA at 12:12, 9 June 2021

OCA: Protected "7csc" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7csc is ON HOLD Authors: Shao, K., Zhang, P. Description: AtPrR1 with NADP+ and (-)pinoresinol Category: Unreleased Structures [[Category: Shao...