7cu5 - Revision history

OCA at 16:16, 29 November 2023

2023-11-29T16:16:24Z

←Older revision		Revision as of 16:16, 29 November 2023
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-	====	+	==N-Glycosylation of PD-1 and glycosylation dependent binding of PD-1 specific monoclonal antibody camrelizumab==
-	<StructureSection load='7cu5' size='340' side='right'caption='[[7cu5]]' scene=''>	+	<StructureSection load='7cu5' size='340' side='right'caption='[[7cu5]], [[Resolution\|resolution]] 2.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cu5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CU5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cu5 OCA], [https://pdbe.org/7cu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cu5 RCSB], [https://www.ebi.ac.uk/pdbsum/7cu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cu5 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.81Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cu5 OCA], [https://pdbe.org/7cu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cu5 RCSB], [https://www.ebi.ac.uk/pdbsum/7cu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cu5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/PDCD1_HUMAN PDCD1_HUMAN] Systemic lupus erythematosus;Multiple sclerosis. Systemic lupus erythematosus 2 (SLEB2) [MIM:[https://omim.org/entry/605218 605218]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.<ref>PMID:12402038</ref>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PDCD1_HUMAN PDCD1_HUMAN] Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.<ref>PMID:21276005</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells. Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors. However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment. Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1. The structure of the camrelizumab/PD-1 complex reveals that camrelizumab mainly utilizes its heavy chain to bind to PD-1, while the light chain sterically inhibits the binding of PD-L1 to PD-1. Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1. These results increase our understanding of how glycosylation affects the activity of PD-1-specific MAbs during immune checkpoint therapy.
		+
		+	N-glycosylation of PD-1 promotes binding of camrelizumab.,Liu K, Tan S, Jin W, Guan J, Wang Q, Sun H, Qi J, Yan J, Chai Y, Wang Z, Deng C, Gao GF EMBO Rep. 2020 Dec 3;21(12):e51444. doi: 10.15252/embr.202051444. Epub 2020 Oct , 15. PMID:33063473<ref>PMID:33063473</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cu5" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cell death protein 3D structures\|Cell death protein 3D structures]]
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Chai Y]]
		+	[[Category: Chu XD]]
		+	[[Category: Gao GF]]
		+	[[Category: Guan JW]]
		+	[[Category: Jin WJ]]
		+	[[Category: Liu KF]]
		+	[[Category: Qi JX]]
		+	[[Category: Sun H]]
		+	[[Category: Tan SG]]
		+	[[Category: Wang WL]]
		+	[[Category: Wang ZF]]
		+	[[Category: Yan JH]]

OCA at 10:48, 31 March 2021

2021-03-31T10:48:02Z

←Older revision		Revision as of 10:48, 31 March 2021
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	<StructureSection load='7cu5' size='340' side='right'caption='[[7cu5]]' scene=''>		<StructureSection load='7cu5' size='340' side='right'caption='[[7cu5]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7cu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cu5 OCA], [~~http~~://pdbe.org/7cu5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7cu5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7cu5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7cu5 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cu5 OCA], [https://pdbe.org/7cu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cu5 RCSB], [https://www.ebi.ac.uk/pdbsum/7cu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cu5 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 05:52, 14 October 2020

2020-10-14T05:52:36Z

←Older revision		Revision as of 05:52, 14 October 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7cu5 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7cu5' size='340' side='right'caption='[[7cu5]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7cu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cu5 OCA], [http://pdbe.org/7cu5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7cu5 RCSB], [http://www.ebi.ac.uk/pdbsum/7cu5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7cu5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:33, 7 October 2020

2020-10-07T04:33:09Z

←Older revision		Revision as of 04:33, 7 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cu5 is ON HOLD	+	The entry 7cu5 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7cu5" [edit=sysop:move=sysop]

2020-08-26T09:30:19Z

Protected "7cu5" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:30, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7cu5 is ON HOLD Authors: Description: Category: Unreleased Structures

2020-08-26T09:30:17Z

New page: '''Unreleased structure''' The entry 7cu5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7cu5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]