7cui - Revision history

OCA at 18:53, 7 September 2022

2022-09-07T18:53:18Z

←Older revision		Revision as of 18:53, 7 September 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO]] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>		[[https://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO]] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The conserved shelterin complex caps chromosome ends to protect telomeres and regulate telomere replication. In fission yeast Schizosaccharomyces pombe, shelterin consists of telomeric single- and double-stranded DNA-binding modules Pot1-Tpz1 and Taz1-Rap1 connected by Poz1, and a specific component Ccq1. While individual structures of the two DNA-binding OB folds of Pot1 (Pot1OB1-GGTTAC and Pot1OB2-GGTTACGGT) are available, structural insight into recognition of telomeric repeats with spacers by the complete DNA-binding domain (Pot1DBD) remains an open question. Moreover, structural information about the Tpz1-Ccq1 interaction requires to be revealed for understanding how the specific component Ccq1 of S. pombe shelterin is recruited to telomeres to function as an interacting hub. Here, we report the crystal structures of Pot1DBD-single-stranded-DNA, Pot1372-555-Tpz1185-212 and Tpz1425-470-Ccq1123-439 complexes and propose an integrated model depicting the assembly mechanism of the shelterin complex at telomeres. The structure of Pot1DBD-DNA unveils how Pot1 recognizes S. pombe degenerate telomeric sequences. Our analyses of Tpz1-Ccq1 reveal structural basis for the essential role of the Tpz1-Ccq1 interaction in telomere recruitment of Ccq1 that is required for telomere maintenance and telomeric heterochromatin formation. Overall, our findings provide valuable structural information regarding interactions within fission yeast shelterin complex at 3' ss telomeric overhang.
		+
		+	Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.,Sun H, Wu Z, Zhou Y, Lu Y, Lu H, Chen H, Shi S, Zeng Z, Wu J, Lei M PLoS Genet. 2022 Jul 18;18(7):e1010308. doi: 10.1371/journal.pgen.1010308., eCollection 2022 Jul. PMID:35849625<ref>PMID:35849625</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cui" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:46, 7 September 2022

2022-09-07T16:46:28Z

←Older revision		Revision as of 16:46, 7 September 2022
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-	====	+	==Crystal structure of fission yeast Pot1 and Tpz1==
-	<StructureSection load='7cui' size='340' side='right'caption='[[7cui]]' scene=''>	+	<StructureSection load='7cui' size='340' side='right'caption='[[7cui]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cui]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CUI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cui OCA], [https://pdbe.org/7cui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cui RCSB], [https://www.ebi.ac.uk/pdbsum/7cui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cui ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cui OCA], [https://pdbe.org/7cui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cui RCSB], [https://www.ebi.ac.uk/pdbsum/7cui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cui ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO]] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Schizosaccharomyces pombe 972h-]]
		+	[[Category: Lei M]]
		+	[[Category: Sun H]]
		+	[[Category: Wu J]]
		+	[[Category: Wu Z]]

OCA at 06:17, 25 August 2021

2021-08-25T06:17:53Z

←Older revision		Revision as of 06:17, 25 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7cui ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7cui' size='340' side='right'caption='[[7cui]]' scene=''>
-	~~Authors~~: ~~Sun, H~~.~~, Wu, Z~~.~~, Wu, J~~.~~, Lei, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal~~ structure ~~of fission yeast Pot1 and Tpz1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cui OCA], [https://pdbe.org/7cui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cui RCSB], [https://www.ebi.ac.uk/pdbsum/7cui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cui ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures]~~]	+	</table>
-	[[~~Category~~: Wu, J]]	+	__TOC__
-	[~~[Category~~: ~~Sun, H~~]]	+	</StructureSection>
-	[[Category: ~~Wu, Z~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lei, M~~]]	+	[[Category: Z-disk]]

OCA at 06:49, 16 September 2020

2020-09-16T06:49:37Z

←Older revision		Revision as of 06:49, 16 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cui is ON HOLD	+	The entry 7cui is ON HOLD until Paper Publication

	Authors: Sun, H., Wu, Z., Wu, J., Lei, M.		Authors: Sun, H., Wu, Z., Wu, J., Lei, M.
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	Description: Crystal structure of fission yeast Pot1 and Tpz1		Description: Crystal structure of fission yeast Pot1 and Tpz1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wu, Z]]
-	[[Category: Lei, M]]
	[[Category: Wu, J]]		[[Category: Wu, J]]
	[[Category: Sun, H]]		[[Category: Sun, H]]
		+	[[Category: Wu, Z]]
		+	[[Category: Lei, M]]

OCA: Protected "7cui" [edit=sysop:move=sysop]

2020-09-02T05:12:55Z

Protected "7cui" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:12, 2 September 2020

OCA: New page: '''Unreleased structure''' The entry 7cui is ON HOLD Authors: Sun, H., Wu, Z., Wu, J., Lei, M. Description: Crystal structure of fission yeast Pot1 and Tpz1 [[Category: Unreleased Stru...

2020-09-02T05:12:54Z

New page: '''Unreleased structure''' The entry 7cui is ON HOLD Authors: Sun, H., Wu, Z., Wu, J., Lei, M. Description: Crystal structure of fission yeast Pot1 and Tpz1 [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 7cui is ON HOLD

Authors: Sun, H., Wu, Z., Wu, J., Lei, M.

Description: Crystal structure of fission yeast Pot1 and Tpz1
[[Category: Unreleased Structures]]
[[Category: Wu, Z]]
[[Category: Lei, M]]
[[Category: Wu, J]]
[[Category: Sun, H]]