7d5q - Revision history

OCA at 16:27, 29 November 2023

2023-11-29T16:27:18Z

←Older revision		Revision as of 16:27, 29 November 2023
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-	====	+	==Structure of NorC transporter (K398A mutant) in an outward-open conformation in complex with a single-chain Indian camelid antibody==
-	<StructureSection load='7d5q' size='340' side='right'caption='[[7d5q]]' scene=''>	+	<StructureSection load='7d5q' size='340' side='right'caption='[[7d5q]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7d5q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D5Q FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d5q OCA], [https://pdbe.org/7d5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d5q RCSB], [https://www.ebi.ac.uk/pdbsum/7d5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d5q ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d5q OCA], [https://pdbe.org/7d5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d5q RCSB], [https://www.ebi.ac.uk/pdbsum/7d5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d5q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H2WZS4_STAAC A0A0H2WZS4_STAAC]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transporters play vital roles in acquiring antimicrobial resistance among pathogenic bacteria. In this study, we report the X-ray structure of NorC, a 14-transmembrane major facilitator superfamily member that is implicated in fluoroquinolone resistance in drug-resistant Staphylococcus aureus strains, at a resolution of 3.6 A. The NorC structure was determined in complex with a single-domain camelid antibody that interacts at the extracellular face of the transporter and stabilizes it in an outward-open conformation. The complementarity determining regions of the antibody enter and block solvent access to the interior of the vestibule, thereby inhibiting alternating-access. NorC specifically interacts with an organic cation, tetraphenylphosphonium, although it does not demonstrate an ability to transport it. The interaction is compromised in the presence of NorC-antibody complex, consequently establishing a strategy to detect and block NorC and related transporters through the use of single-domain camelid antibodies.
		+
		+	Structural basis of inhibition of a transporter from Staphylococcus aureus, NorC, through a single-domain camelid antibody.,Kumar S, Athreya A, Gulati A, Nair RM, Mahendran I, Ranjan R, Penmatsa A Commun Biol. 2021 Jul 5;4(1):836. doi: 10.1038/s42003-021-02357-x. PMID:34226658<ref>PMID:34226658</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7d5q" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus dromedarius]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Staphylococcus aureus subsp. aureus COL]]
		+	[[Category: Athreya A]]
		+	[[Category: Kumar S]]
		+	[[Category: Penmatsa A]]

OCA at 12:13, 9 June 2021

2021-06-09T12:13:04Z

←Older revision		Revision as of 12:13, 9 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7d5q ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7d5q' size='340' side='right'caption='[[7d5q]]' scene=''>
-	~~Authors~~: ~~Kumar, S~~.~~, Athreya, A~~.~~, Penmatsa, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Structure of NorC transporter (K398A mutant) in an outward~~-~~open conformation in complex with~~ a ~~single-chain Indian camelid antibody~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d5q OCA], [https://pdbe.org/7d5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d5q RCSB], [https://www.ebi.ac.uk/pdbsum/7d5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d5q ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Athreya~~, A]]	+	__TOC__
-	[[Category: ~~Penmatsa, A~~]]	+	</StructureSection>
-	[[Category: ~~Kumar, S~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:10, 14 October 2020

2020-10-14T04:10:27Z

←Older revision		Revision as of 04:10, 14 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7d5q is ON HOLD	+	The entry 7d5q is ON HOLD until Paper Publication

	Authors: Kumar, S., Athreya, A., Penmatsa, A.		Authors: Kumar, S., Athreya, A., Penmatsa, A.

OCA: Protected "7d5q" [edit=sysop:move=sysop]

2020-10-07T04:37:01Z

Protected "7d5q" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:37, 7 October 2020

OCA: New page: '''Unreleased structure''' The entry 7d5q is ON HOLD Authors: Kumar, S., Athreya, A., Penmatsa, A. Description: Structure of NorC transporter (K398A mutant) in an outward-open conforma...

2020-10-07T04:36:59Z

New page: '''Unreleased structure''' The entry 7d5q is ON HOLD Authors: Kumar, S., Athreya, A., Penmatsa, A. Description: Structure of NorC transporter (K398A mutant) in an outward-open conforma...

New page

'''Unreleased structure'''

The entry 7d5q is ON HOLD

Authors: Kumar, S., Athreya, A., Penmatsa, A.

Description: Structure of NorC transporter (K398A mutant) in an outward-open conformation in complex with a single-chain Indian camelid antibody
[[Category: Unreleased Structures]]
[[Category: Athreya, A]]
[[Category: Penmatsa, A]]
[[Category: Kumar, S]]