7d93 - Revision history

OCA at 16:28, 29 November 2023

2023-11-29T16:28:43Z

←Older revision		Revision as of 16:28, 29 November 2023
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-	====	+	==Crystal Structure of the Na+,K+-ATPase in the E2P state with bound Mg2+ and anthroylouabain (P2(1)2(1)2(1) symmetry)==
-	<StructureSection load='7d93' size='340' side='right'caption='[[7d93]]' scene=''>	+	<StructureSection load='7d93' size='340' side='right'caption='[[7d93]], [[Resolution\|resolution]] 3.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7d93]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D93 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d93 OCA], [https://pdbe.org/7d93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d93 RCSB], [https://www.ebi.ac.uk/pdbsum/7d93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d93 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=H0C:[(2~{R},3~{R},4~{R},5~{S},6~{S})-2-[[(1~{R},3~{S},5~{S},8~{R},9~{S},10~{R},11~{R},13~{R},14~{S},17~{R})-10-(hydroxymethyl)-13-methyl-1,5,11,14-tetrakis(oxidanyl)-17-(5-oxidanylidene-2~{H}-furan-3-yl)-2,3,4,6,7,8,9,11,12,15,16,17-dodecahydro-1~{H}-cyclopenta[a]phenanthren-3-yl]oxy]-6-methyl-3,5-bis(oxidanyl)oxan-4-yl]+anthracene-9-carboxylate'>H0C</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCW:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PCW</scene>, <scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d93 OCA], [https://pdbe.org/7d93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d93 RCSB], [https://www.ebi.ac.uk/pdbsum/7d93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d93 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AT1A1_PIG AT1A1_PIG] This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The sodium pump (Na(+), K(+)-ATPase, NKA) is vital for animal cells, as it actively maintains Na(+) and K(+) electrochemical gradients across the cell membrane. It is a target of cardiotonic steroids (CTSs) such as ouabain and digoxin. As CTSs are almost unique strong inhibitors specific to NKA, a wide range of derivatives has been developed for potential therapeutic use. Several crystal structures have been published for NKA-CTS complexes, but they fail to explain the largely different inhibitory properties of the various CTSs. For instance, although CTSs are thought to inhibit ATPase activity by binding to NKA in the E2P state, we do not know if large conformational changes accompany binding, as no crystal structure is available for the E2P state free of CTS. Here, we describe crystal structures of the BeF(3)(-) complex of NKA representing the E2P ground state and then eight crystal structures of seven CTSs, including rostafuroxin and istaroxime, two new members under clinical trials, in complex with NKA in the E2P state. The conformations of NKA are virtually identical in all complexes with and without CTSs, showing that CTSs bind to a preformed cavity in NKA. By comparing the inhibitory potency of the CTSs measured under four different conditions, we elucidate how different structural features of the CTSs result in different inhibitory properties. The crystal structures also explain K(+)-antagonism and suggest a route to isoform specific CTSs.
		+
		+	Binding of cardiotonic steroids to Na(+),K(+)-ATPase in the E2P state.,Kanai R, Cornelius F, Ogawa H, Motoyama K, Vilsen B, Toyoshima C Proc Natl Acad Sci U S A. 2021 Jan 7;118(1):e2020438118. doi: , 10.1073/pnas.2020438118. PMID:33318128<ref>PMID:33318128</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7d93" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase 3D structures\|ATPase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Sus scrofa]]
		+	[[Category: Cornelius F]]
		+	[[Category: Kanai R]]
		+	[[Category: Motoyama K]]
		+	[[Category: Ogawa H]]
		+	[[Category: Toyoshima C]]
		+	[[Category: Vilsen B]]

OCA at 07:57, 27 January 2021

2021-01-27T07:57:46Z

←Older revision		Revision as of 07:57, 27 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7d93 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7d93' size='340' side='right'caption='[[7d93]]' scene=''>
-	~~Authors~~: ~~Kanai, R~~.~~, Cornelius, F~~.~~, Ogawa, H~~.~~, Motoyama, K~~.~~, Vilsen, B~~.~~, Toyoshima, C~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure of the Na+,K+-ATPase in the E2P state with bound Mg2+ and anthroylouabain (P2(1)2(1)2(1) symmetry)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d93 OCA], [https://pdbe.org/7d93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d93 RCSB], [https://www.ebi.ac.uk/pdbsum/7d93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d93 ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Kanai, R~~]]	+	__TOC__
-	[~~[Category~~: ~~Motoyama, K~~]]	+	</StructureSection>
-	[~~[Category~~: ~~Toyoshima, C~~]]	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Cornelius~~, F]]	+	[[Category: Z-disk]]
-	[[Category: ~~Ogawa, H~~]]	+
-	[[Category: ~~Vilsen, B~~]]	+

OCA at 06:24, 11 November 2020

2020-11-11T06:24:09Z

←Older revision		Revision as of 06:24, 11 November 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7d93 is ON HOLD	+	The entry 7d93 is ON HOLD until Paper Publication

	Authors: Kanai, R., Cornelius, F., Ogawa, H., Motoyama, K., Vilsen, B., Toyoshima, C.		Authors: Kanai, R., Cornelius, F., Ogawa, H., Motoyama, K., Vilsen, B., Toyoshima, C.

OCA: Protected "7d93" [edit=sysop:move=sysop]

2020-10-21T06:44:47Z

Protected "7d93" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 21 October 2020

OCA: New page: '''Unreleased structure''' The entry 7d93 is ON HOLD Authors: Kanai, R., Cornelius, F., Ogawa, H., Motoyama, K., Vilsen, B., Toyoshima, C. Description: Crystal Structure of the Na+,K+-...

2020-10-21T06:44:46Z

New page: '''Unreleased structure''' The entry 7d93 is ON HOLD Authors: Kanai, R., Cornelius, F., Ogawa, H., Motoyama, K., Vilsen, B., Toyoshima, C. Description: Crystal Structure of the Na+,K+-...

New page

'''Unreleased structure'''

The entry 7d93 is ON HOLD

Authors: Kanai, R., Cornelius, F., Ogawa, H., Motoyama, K., Vilsen, B., Toyoshima, C.

Description: Crystal Structure of the Na+,K+-ATPase in the E2P state with bound Mg2+ and anthroylouabain (P2(1)2(1)2(1) symmetry)
[[Category: Unreleased Structures]]
[[Category: Kanai, R]]
[[Category: Motoyama, K]]
[[Category: Toyoshima, C]]
[[Category: Cornelius, F]]
[[Category: Ogawa, H]]
[[Category: Vilsen, B]]