7dl1 - Revision history

OCA at 19:32, 29 May 2024

2024-05-29T19:32:07Z

←Older revision		Revision as of 19:32, 29 May 2024
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	==The mutant E310G/G323S structure of 3,5-DAHDHcca complex with NADPH==		==The mutant E310G/G323S structure of 3,5-DAHDHcca complex with NADPH==
-	<StructureSection load='7dl1' size='340' side='right'caption='[[7dl1]]' scene=''>	+	<StructureSection load='7dl1' size='340' side='right'caption='[[7dl1]], [[Resolution\|resolution]] 2.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DL1 FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DL1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dl1 OCA], [https://pdbe.org/7dl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dl1 RCSB], [https://www.ebi.ac.uk/pdbsum/7dl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dl1 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.72Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dl1 OCA], [https://pdbe.org/7dl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dl1 RCSB], [https://www.ebi.ac.uk/pdbsum/7dl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dl1 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Amino acid dehydrogenases (AADHs) have shown considerable potential as biocatalysts in the asymmetric synthesis of chiral amino acids. However, compared to the widely studied alpha-AADHs, limited knowledge is available about beta-AADHs that enable the synthesis of beta-amino acids. Herein, we report the crystal structures of a l-erythro-3,5-diaminohexanoate dehydrogenase and its variants, the only known member of beta-AADH family. Crystal structure analysis, site-directed mutagenesis studies and quantum chemical calculations revealed the differences in the substrate binding and catalytic mechanism from alpha-AADHs. A number of rationally engineered variants were then obtained with improved activity (by 110-800 times) toward various aliphatic beta-amino acids without an enantioselectivity trade-off. Two beta-amino acids were prepared by using the outstanding variants with excellent enantioselectivity (>99 % ee) and high isolated yields (86-87 %). These results provide important insights into the molecular mechanism of 3,5-DAHDH, and establish a solid foundation for further design of beta-AADHs for the asymmetric synthesis of beta-amino acids.
		+
		+	Crystal Structures and Catalytic Mechanism of l-erythro-3,5-Diaminohexanoate Dehydrogenase and Rational Engineering for Asymmetric Synthesis of beta-Amino Acids.,Liu N, Wu L, Feng J, Sheng X, Li J, Chen X, Li J, Liu W, Zhou J, Wu Q, Zhu D Angew Chem Int Ed Engl. 2021 Apr 26;60(18):10203-10210. doi:, 10.1002/anie.202017225. Epub 2021 Mar 24. PMID:33624917<ref>PMID:33624917</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7dl1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:48, 29 September 2021

2021-09-29T08:48:12Z

←Older revision		Revision as of 08:48, 29 September 2021
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-	'''Unreleased structure'''

-	The ~~entry~~ 7dl1 is ~~ON HOLD until Nov 25 2022~~	+	==The mutant E310G/G323S structure of 3,5-DAHDHcca complex with NADPH==
-		+	<StructureSection load='7dl1' size='340' side='right'caption='[[7dl1]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DL1 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dl1 OCA], [https://pdbe.org/7dl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dl1 RCSB], [https://www.ebi.ac.uk/pdbsum/7dl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dl1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Liu N]]
		+	[[Category: Wu L]]
		+	[[Category: Zhou JH]]
		+	[[Category: Zhu DM]]

OCA at 07:19, 8 September 2021

2021-09-08T07:19:17Z

←Older revision		Revision as of 07:19, 8 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7dl1 is ON HOLD	+	The entry 7dl1 is ON HOLD until Nov 25 2022

	Authors:		Authors:

OCA: Protected "7dl1" [edit=sysop:move=sysop]

2020-12-02T05:44:19Z

Protected "7dl1" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:44, 2 December 2020

OCA: New page: '''Unreleased structure''' The entry 7dl1 is ON HOLD Authors: Description: Category: Unreleased Structures

2020-12-02T05:44:17Z

New page: '''Unreleased structure''' The entry 7dl1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7dl1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]