OCA at 10:53, 27 March 2024

2024-03-27T10:53:45Z

←Older revision		Revision as of 10:53, 27 March 2024
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-	====	+	==Cryo-EM structure of LshCas13a-crRNA-anti-tag RNA complex==
-	<StructureSection load='7dmq' size='340' side='right'caption='[[7dmq]]' scene=''>	+	<StructureSection load='7dmq' size='340' side='right'caption='[[7dmq]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7dmq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Leptotrichia_shahii Leptotrichia shahii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DMQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DMQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dmq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dmq OCA], [https://pdbe.org/7dmq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dmq RCSB], [https://www.ebi.ac.uk/pdbsum/7dmq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dmq ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.06Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dmq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dmq OCA], [https://pdbe.org/7dmq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dmq RCSB], [https://www.ebi.ac.uk/pdbsum/7dmq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dmq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CS13A_LEPSD CS13A_LEPSD] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements (spacers) and target invading nucleic acids. Unlike many single-component effectors, this CRISPR-Cas system targets RNA (PubMed:27256883, PubMed:28086085). CRISPR clusters are transcribed from pre-CRISPR RNA (crRNA) and processed into crRNA (optimally 28 nucleotides in this system) by this protein (PubMed:27256883, PubMed:27669025, PubMed:28475872, PubMed:28086085). This protein processes pre-crRNA at a 'non-typical' site 1 nucleotide upstream of the pre-crRNA stem-loop; it cleaves pre-crRNA from L.buccalis and L.wadei in a similar fashion, whereas the enzymes from the latter 2 bacteria cleave their own pre-crRNA 3 nt further upstream (PubMed:28475872). When the appropriate target sequences are cloned into the CRISPR array, confers immunity to ssRNA(+) enterobacteria phage MS2 (PubMed:27256883). Cleaves linear target ssRNA in a crRNA-dependent fashion, preferentially before U residues; has no activity on partially dsRNA, ssDNA or dsDNA (PubMed:27256883). RNA secondary structure surrounding the target influence the cleavage site and efficiency; unlike other CRISPR-Cas effectors Cas13a cleaves outside of the crRNA binding site (PubMed:27256883). In the presence of a viable RNA target other RNAs are also degraded (called collateral RNA degradation), suggesting this type of CRISPR-Cas might also prevent viral spread by inducing programmed cell death or dormancy (PubMed:27256883). This system has a 3' protospacer flanking site (PFS), it does not cleave when the 3' PFS is G (PFS is equivalent to PAM, the protospacer adjacent motif) (PubMed:27256883). Mutations of its active site residues results in an RNA-programmed RNA-binding protein (PubMed:27256883).<ref>PMID:27256883</ref> <ref>PMID:27669025</ref> <ref>PMID:28086085</ref> <ref>PMID:28475872</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Leptotrichia shahii]]
		+	[[Category: Ding J]]
		+	[[Category: Patel DJ]]
		+	[[Category: Wang B]]
		+	[[Category: Yang H]]
		+	[[Category: Zhang T]]

OCA at 11:03, 10 February 2021

2021-02-10T11:03:09Z

←Older revision		Revision as of 11:03, 10 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7dmq ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7dmq' size='340' side='right'caption='[[7dmq]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dmq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dmq OCA], [https://pdbe.org/7dmq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dmq RCSB], [https://www.ebi.ac.uk/pdbsum/7dmq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dmq ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:35, 6 January 2021

2021-01-06T05:35:45Z

←Older revision		Revision as of 05:35, 6 January 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7dmq is ON HOLD	+	The entry 7dmq is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7dmq" [edit=sysop:move=sysop]

2020-12-16T11:06:57Z

Protected "7dmq" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:06, 16 December 2020

OCA: New page: '''Unreleased structure''' The entry 7dmq is ON HOLD Authors: Description: Category: Unreleased Structures

2020-12-16T11:06:56Z

New page: '''Unreleased structure''' The entry 7dmq is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7dmq is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7dmq - Revision history

OCA at 10:53, 27 March 2024

OCA at 11:03, 10 February 2021

OCA at 05:35, 6 January 2021

OCA: Protected "7dmq" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7dmq is ON HOLD Authors: Description: Category: Unreleased Structures