OCA at 16:44, 29 November 2023

2023-11-29T16:44:53Z

←Older revision		Revision as of 16:44, 29 November 2023
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-	====	+	==Crystal structure of Dehydrogenase/isomerase FabX from Helicobacter pylori in complex with holo-ACP==
-	<StructureSection load='7e1r' size='340' side='right'caption='[[7e1r]]' scene=''>	+	<StructureSection load='7e1r' size='340' side='right'caption='[[7e1r]], [[Resolution\|resolution]] 2.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7e1r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E1R FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e1r OCA], [https://pdbe.org/7e1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e1r RCSB], [https://www.ebi.ac.uk/pdbsum/7e1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e1r ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.795Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PN7:N~3~-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-N-(2-SULFANYLETHYL)-BETA-ALANINAMIDE'>PN7</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e1r OCA], [https://pdbe.org/7e1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e1r RCSB], [https://www.ebi.ac.uk/pdbsum/7e1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e1r ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B5Z7D6_HELPG B5Z7D6_HELPG]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Unsaturated fatty acids (UFAs) are essential for functional membrane phospholipids in most bacteria. The bifunctional dehydrogenase/isomerase FabX is an essential UFA biosynthesis enzyme in the widespread human pathogen Helicobacter pylori, a bacterium etiologically related to 95% of gastric cancers. Here, we present the crystal structures of FabX alone and in complexes with an octanoyl-acyl carrier protein (ACP) substrate or with holo-ACP. FabX belongs to the nitronate monooxygenase (NMO) flavoprotein family but contains an atypical [4Fe-4S] cluster absent in all other family members characterized to date. FabX binds ACP via its positively charged alpha7 helix that interacts with the negatively charged alpha2 and alpha3 helices of ACP. We demonstrate that the [4Fe-4S] cluster potentiates FMN oxidation during dehydrogenase catalysis, generating superoxide from an oxygen molecule that is locked in an oxyanion hole between the FMN and the active site residue His182. Both the [4Fe-4S] and FMN cofactors are essential for UFA synthesis, and the superoxide is subsequently excreted by H. pylori as a major resource of peroxide which may contribute to its pathogenic function in the corrosion of gastric mucosa.
		+
		+	Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis.,Zhou J, Zhang L, Zeng L, Yu L, Duan Y, Shen S, Hu J, Zhang P, Song W, Ruan X, Jiang J, Zhang Y, Zhou L, Jia J, Hang X, Tian C, Lin H, Chen HZ, Cronan JE, Bi H, Zhang L Nat Commun. 2021 Nov 26;12(1):6932. doi: 10.1038/s41467-021-27148-0. PMID:34836944<ref>PMID:34836944</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7e1r" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Helicobacter pylori]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Zhang L]]
		+	[[Category: Zhou JS]]

OCA at 06:18, 1 December 2021

2021-12-01T06:18:21Z

←Older revision		Revision as of 06:18, 1 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7e1r ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7e1r' size='340' side='right'caption='[[7e1r]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e1r OCA], [https://pdbe.org/7e1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e1r RCSB], [https://www.ebi.ac.uk/pdbsum/7e1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e1r ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:32, 24 February 2021

2021-02-24T05:32:24Z

←Older revision		Revision as of 05:32, 24 February 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7e1r is ON HOLD	+	The entry 7e1r is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7e1r" [edit=sysop:move=sysop]

2021-02-10T09:10:17Z

Protected "7e1r" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:10, 10 February 2021

OCA: New page: '''Unreleased structure''' The entry 7e1r is ON HOLD Authors: Description: Category: Unreleased Structures

2021-02-10T09:10:16Z

New page: '''Unreleased structure''' The entry 7e1r is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7e1r is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7e1r - Revision history

OCA at 16:44, 29 November 2023

OCA at 06:18, 1 December 2021

OCA at 05:32, 24 February 2021

OCA: Protected "7e1r" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7e1r is ON HOLD Authors: Description: Category: Unreleased Structures