7e36 - Revision history

OCA at 16:45, 29 November 2023

2023-11-29T16:45:31Z

←Older revision		Revision as of 16:45, 29 November 2023
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-	====	+	==A [6+4]-cycloaddition adduct is the biosynthetic intermediate in streptoseomycin biosynthesis==
-	<StructureSection load='7e36' size='340' side='right'caption='[[7e36]]' scene=''>	+	<StructureSection load='7e36' size='340' side='right'caption='[[7e36]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7e36]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_tenerifensis Nocardia tenerifensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E36 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E36 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e36 OCA], [https://pdbe.org/7e36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e36 RCSB], [https://www.ebi.ac.uk/pdbsum/7e36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e36 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e36 OCA], [https://pdbe.org/7e36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e36 RCSB], [https://www.ebi.ac.uk/pdbsum/7e36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e36 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A318JWT9_9NOCA A0A318JWT9_9NOCA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptoseomycin (STM, 1) is a bacterial macrolactone that has a unique 5/14/10/6/6-pentacyclic ring with an ether bridge. We have previously identified the biosynthetic gene cluster for 1 and characterized StmD as [6 + 4]- and [4 + 2]-bispericyclase that catalyze a reaction leading to both 6/10/6- and 10/6/6-tricyclic adducts (6 and 7). The remaining steps, especially how to install and stabilize the required 10/6/6-tricyclic core for downstream modifications, remain unknown. In this work, we have identified three oxidoreductases that fix the required 10/6/6-tryciclic core. A pair of flavin-dependent oxidoreductases, StmO1 and StmO2, catalyze the direct hydroxylation at [6 + 4]-adduct (6). Subsequently, a spontaneous [3,3]-Cope rearrangement and an enol-ketone tautomerization result in the formation of 10/6/6-tricyclic intermediate 12b, which can be further converted to a stable 10/6/6-tricyclic alcohol 11 through a ketoreduction by StmK. Crystal structure of the heterodimeric complex NtfO1-NtfO2, homologues of StmO1-StmO2 with equivalent function, reveals protein-protein interactions. Our results demonstrate that the [6 + 4]-adduct instead of [4 + 2]-adduct is the bona fide biosynthetic intermediate.
		+
		+	A [6+4]-cycloaddition adduct is the biosynthetic intermediate in streptoseomycin biosynthesis.,Wang KB, Wang W, Zhang B, Wang X, Chen Y, Zhu HJ, Liang Y, Tan RX, Ge HM Nat Commun. 2021 Apr 7;12(1):2092. doi: 10.1038/s41467-021-22395-7. PMID:33828077<ref>PMID:33828077</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7e36" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Nocardia tenerifensis]]
		+	[[Category: Ge HM]]
		+	[[Category: Zhang B]]

OCA at 17:20, 10 March 2021

2021-03-10T17:20:28Z

←Older revision		Revision as of 17:20, 10 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7e36 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7e36' size='340' side='right'caption='[[7e36]]' scene=''>
-	~~Authors~~: ~~Zhang, B~~.~~, Ge, H~~.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Oxidation, Cope Rearrangement~~, ~~Tautomerization and Reduction Cascades Install 10~~/6/6-~~tricyclic Core in Streptoseomycin~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e36 OCA], [https://pdbe.org/7e36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e36 RCSB], [https://www.ebi.ac.uk/pdbsum/7e36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e36 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Zhang, B~~]]	+	__TOC__
-	[[Category: ~~Ge, H.M~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:32, 24 February 2021

2021-02-24T05:32:54Z

←Older revision		Revision as of 05:32, 24 February 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7e36 is ON HOLD	+	The entry 7e36 is ON HOLD until Paper Publication

	Authors: Zhang, B., Ge, H.M.		Authors: Zhang, B., Ge, H.M.

OCA: Protected "7e36" [edit=sysop:move=sysop]

2021-02-17T08:37:32Z

Protected "7e36" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:37, 17 February 2021

OCA: New page: '''Unreleased structure''' The entry 7e36 is ON HOLD Authors: Zhang, B., Ge, H.M. Description: Oxidation, Cope Rearrangement, Tautomerization and Reduction Cascades Install 10/6/6-tric...

2021-02-17T08:37:31Z

New page: '''Unreleased structure''' The entry 7e36 is ON HOLD Authors: Zhang, B., Ge, H.M. Description: Oxidation, Cope Rearrangement, Tautomerization and Reduction Cascades Install 10/6/6-tric...

New page

'''Unreleased structure'''

The entry 7e36 is ON HOLD

Authors: Zhang, B., Ge, H.M.

Description: Oxidation, Cope Rearrangement, Tautomerization and Reduction Cascades Install 10/6/6-tricyclic Core in Streptoseomycin
[[Category: Unreleased Structures]]
[[Category: Zhang, B]]
[[Category: Ge, H.M]]