7e3f - Revision history

OCA at 16:45, 29 November 2023

2023-11-29T16:45:39Z

←Older revision		Revision as of 16:45, 29 November 2023
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	==Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant==		==Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant==
-	<StructureSection load='7e3f' size='340' side='right'caption='[[7e3f]]' scene=''>	+	<StructureSection load='7e3f' size='340' side='right'caption='[[7e3f]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7e3f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3F FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3f OCA], [https://pdbe.org/7e3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3f RCSB], [https://www.ebi.ac.uk/pdbsum/7e3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3f ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3f OCA], [https://pdbe.org/7e3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3f RCSB], [https://www.ebi.ac.uk/pdbsum/7e3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3f ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D6XHE1_TRYB2 D6XHE1_TRYB2]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein assemblies can be designed for development of nano-bio materials. This has been achieved by modulating protein-protein interactions. However, fabrication of highly ordered protein assemblies remains challenging. Protein crystals, which have highly ordered arrangements of protein molecules, provide useful source matrices for synthesizing artificial protein assemblies. Here, we describe construction of a supramolecular filament structure by engineering covalent and non-covalent interactions in a protein crystal. Performing in-cell crystallization of Trypanosoma brucei cysteine protease cathepsin B (TbCatB), we achieved a precise arrangement of protein molecules while suppressing random aggregation due to disulfide bonds. We succeeded in synthesizing bundled filament from the crystals by autoxidation of cysteinyl thiols after the isolation of the crystals from living cells.
		+
		+	Design of an In-Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament.,Abe S, Pham TT, Negishi H, Yamashita K, Hirata K, Ueno T Angew Chem Int Ed Engl. 2021 May 25;60(22):12341-12345. doi:, 10.1002/anie.202102039. Epub 2021 Apr 23. PMID:33759310<ref>PMID:33759310</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7e3f" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cathepsin 3D structures\|Cathepsin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Trypanosoma brucei brucei TREU927]]
	[[Category: Abe S]]		[[Category: Abe S]]
	[[Category: Hirata K]]		[[Category: Hirata K]]

OCA at 16:46, 15 December 2021

2021-12-15T16:46:45Z

←Older revision		Revision as of 16:46, 15 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7e3f ~~is ON HOLD until Feb 08 2023~~	+	==Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant==
-		+	<StructureSection load='7e3f' size='340' side='right'caption='[[7e3f]]' scene=''>
-	~~Authors~~: ~~Abe, S~~., ~~Pham, T~~.T., ~~Negishi, H~~., ~~Yamashita, K~~., ~~Hirata, K~~., ~~Ueno, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3F FirstGlance]. <br>
-	~~Description: Crystal structure of Trypanosoma brucei cathepsin B Y217C~~/~~S275C mutant~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3f OCA], [https://pdbe.org/7e3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3f RCSB], [https://www.ebi.ac.uk/pdbsum/7e3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3f ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Pham, T.T~~]]	+	__TOC__
-	[[Category: ~~Yamashita,~~ K]]	+	</StructureSection>
-	[[Category: Negishi, H]]	+	[[Category: Large Structures]]
-	[[Category: ~~Abe, S~~]]	+	[[Category: Abe S]]
-	[[Category: ~~Hirata, K~~]]	+	[[Category: Hirata K]]
-	[[Category: ~~Ueno, T~~]]	+	[[Category: Negishi H]]
		+	[[Category: Pham TT]]
		+	[[Category: Ueno T]]
		+	[[Category: Yamashita K]]

OCA at 09:13, 31 March 2021

2021-03-31T09:13:42Z

←Older revision		Revision as of 09:13, 31 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7e3f is ON HOLD	+	The entry 7e3f is ON HOLD until Feb 08 2023

	Authors: Abe, S., Pham, T.T., Negishi, H., Yamashita, K., Hirata, K., Ueno, T.		Authors: Abe, S., Pham, T.T., Negishi, H., Yamashita, K., Hirata, K., Ueno, T.

OCA: Protected "7e3f" [edit=sysop:move=sysop]

2021-02-17T08:38:01Z

Protected "7e3f" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:38, 17 February 2021

OCA: New page: '''Unreleased structure''' The entry 7e3f is ON HOLD Authors: Abe, S., Pham, T.T., Negishi, H., Yamashita, K., Hirata, K., Ueno, T. Description: Crystal structure of Trypanosoma brucei...

2021-02-17T08:37:59Z

New page: '''Unreleased structure''' The entry 7e3f is ON HOLD Authors: Abe, S., Pham, T.T., Negishi, H., Yamashita, K., Hirata, K., Ueno, T. Description: Crystal structure of Trypanosoma brucei...

New page

'''Unreleased structure'''

The entry 7e3f is ON HOLD

Authors: Abe, S., Pham, T.T., Negishi, H., Yamashita, K., Hirata, K., Ueno, T.

Description: Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant
[[Category: Unreleased Structures]]
[[Category: Pham, T.T]]
[[Category: Yamashita, K]]
[[Category: Negishi, H]]
[[Category: Abe, S]]
[[Category: Hirata, K]]
[[Category: Ueno, T]]