7eht - Revision history

OCA at 16:53, 29 November 2023

2023-11-29T16:53:56Z

←Older revision		Revision as of 16:53, 29 November 2023
Line 1:		Line 1:

	==Levansucrase from Brenneria sp. EniD 312==		==Levansucrase from Brenneria sp. EniD 312==
-	<StructureSection load='7eht' size='340' side='right'caption='[[7eht]]' scene=''>	+	<StructureSection load='7eht' size='340' side='right'caption='[[7eht]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eht]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brenneria_sp._EniD312 Brenneria sp. EniD312]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eht OCA], [https://pdbe.org/7eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eht RCSB], [https://www.ebi.ac.uk/pdbsum/7eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eht ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eht OCA], [https://pdbe.org/7eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eht RCSB], [https://www.ebi.ac.uk/pdbsum/7eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eht ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G7LSK3_9GAMM G7LSK3_9GAMM]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microbial levansucrases (LSs, EC 2.4.1.10) have been widely studied for the synthesis of beta-(2,6)-fructans (levan) from sucrose. LSs synthesize levan-type fructo-oligosaccharides, high-molecular-mass levan polymer or combinations of both. Here, we report crystal structures of LS from the G(-)-bacterium Brenneria sp. EniD 312 (Brs-LS) in its apo form, as well as of two mutants (A154S, H327A) targeting positions known to affect LS reaction specificity. In addition, we report a structure of Brs-LS complexed with sucrose, the first crystal structure of a G(-)-LS with a bound substrate. The overall structure of Brs-LS is similar to that of G(-)- and G(+)-LSs, with the nucleophile (D68), transition stabilizer (D225), and a general acid/base (E309) in its active site. The H327A mutant lacks an essential interaction with glucosyl moieties of bound substrates in subsite +1, explaining the observed smaller products synthesized by this mutant. The A154S mutation affects the hydrogen-bond network around the transition stabilizing residue (D225) and the nucleophile (D68), and may affect the affinity of the enzyme for sucrose such that it becomes less effective in transfructosylation. Taken together, this study provides novel insights into the roles of structural elements and residues in the product specificity of LSs.
		+
		+	Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity.,Xu W, Ni D, Hou X, Pijning T, Guskov A, Rao Y, Mu W J Agric Food Chem. 2022 Apr 7. doi: 10.1021/acs.jafc.2c01225. PMID:35388691<ref>PMID:35388691</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eht" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Brenneria sp. EniD312]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Guskov A]]		[[Category: Guskov A]]

OCA at 14:43, 31 August 2022

2022-08-31T14:43:41Z

←Older revision		Revision as of 14:43, 31 August 2022
Line 1:		Line 1:

	==Levansucrase from Brenneria sp. EniD 312==		==Levansucrase from Brenneria sp. EniD 312==
-	<StructureSection load='7eht' size='340' side='right'caption='[[7eht]]~~, [[Resolution\|resolution]] 1.45Å~~' scene=''>	+	<StructureSection load='7eht' size='340' side='right'caption='[[7eht]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[7eht]] is a 1 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eht OCA], [https://pdbe.org/7eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eht RCSB], [https://www.ebi.ac.uk/pdbsum/7eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eht ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eht OCA], [https://pdbe.org/7eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eht RCSB], [https://www.ebi.ac.uk/pdbsum/7eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eht ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Microbial levansucrases (LSs, EC 2.4.1.10) have been widely studied for the synthesis of beta-(2,6)-fructans (levan) from sucrose. LSs synthesize levan-type fructo-oligosaccharides, high-molecular-mass levan polymer or combinations of both. Here, we report crystal structures of LS from the G(-)-bacterium Brenneria sp. EniD 312 (Brs-LS) in its apo form, as well as of two mutants (A154S, H327A) targeting positions known to affect LS reaction specificity. In addition, we report a structure of Brs-LS complexed with sucrose, the first crystal structure of a G(-)-LS with a bound substrate. The overall structure of Brs-LS is similar to that of G(-)- and G(+)-LSs, with the nucleophile (D68), transition stabilizer (D225), and a general acid/base (E309) in its active site. The H327A mutant lacks an essential interaction with glucosyl moieties of bound substrates in subsite +1, explaining the observed smaller products synthesized by this mutant. The A154S mutation affects the hydrogen-bond network around the transition stabilizing residue (D225) and the nucleophile (D68), and may affect the affinity of the enzyme for sucrose such that it becomes less effective in transfructosylation. Taken together, this study provides novel insights into the roles of structural elements and residues in the product specificity of LSs.
-
-	Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity.,Xu W, Ni D, Hou X, Pijning T, Guskov A, Rao Y, Mu W J Agric Food Chem. 2022 Apr 7. doi: 10.1021/acs.jafc.2c01225. PMID:35388691<ref>PMID:35388691</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7eht" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Levansucrase]]~~	+	[[Category: Guskov A]]
-	[[Category: Guskov, A]]	+	[[Category: Hou XD]]
-	[[Category: Hou~~, X D~~]]	+	[[Category: Mu WM]]
-	[[Category: Mu~~, W M~~]]	+	[[Category: Pijning T]]
-	[[Category: Pijning, T]]	+	[[Category: Rao YJ]]
-	[[Category: Rao~~, Y J~~]]	+	[[Category: Xu W]]
-	[[Category: Xu, W]]	+
-	~~[[Category: Fructosyltransferase]]~~	+
-	~~[[Category: Levan synthesis]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:48, 27 April 2022

2022-04-27T11:48:22Z

←Older revision		Revision as of 11:48, 27 April 2022
Line 1:		Line 1:

	==Levansucrase from Brenneria sp. EniD 312==		==Levansucrase from Brenneria sp. EniD 312==
-	<StructureSection load='7eht' size='340' side='right'caption='[[7eht]]' scene=''>	+	<StructureSection load='7eht' size='340' side='right'caption='[[7eht]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eht]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eht OCA], [https://pdbe.org/7eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eht RCSB], [https://www.ebi.ac.uk/pdbsum/7eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eht ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eht OCA], [https://pdbe.org/7eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eht RCSB], [https://www.ebi.ac.uk/pdbsum/7eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eht ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microbial levansucrases (LSs, EC 2.4.1.10) have been widely studied for the synthesis of beta-(2,6)-fructans (levan) from sucrose. LSs synthesize levan-type fructo-oligosaccharides, high-molecular-mass levan polymer or combinations of both. Here, we report crystal structures of LS from the G(-)-bacterium Brenneria sp. EniD 312 (Brs-LS) in its apo form, as well as of two mutants (A154S, H327A) targeting positions known to affect LS reaction specificity. In addition, we report a structure of Brs-LS complexed with sucrose, the first crystal structure of a G(-)-LS with a bound substrate. The overall structure of Brs-LS is similar to that of G(-)- and G(+)-LSs, with the nucleophile (D68), transition stabilizer (D225), and a general acid/base (E309) in its active site. The H327A mutant lacks an essential interaction with glucosyl moieties of bound substrates in subsite +1, explaining the observed smaller products synthesized by this mutant. The A154S mutation affects the hydrogen-bond network around the transition stabilizing residue (D225) and the nucleophile (D68), and may affect the affinity of the enzyme for sucrose such that it becomes less effective in transfructosylation. Taken together, this study provides novel insights into the roles of structural elements and residues in the product specificity of LSs.
		+
		+	Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity.,Xu W, Ni D, Hou X, Pijning T, Guskov A, Rao Y, Mu W J Agric Food Chem. 2022 Apr 7. doi: 10.1021/acs.jafc.2c01225. PMID:35388691<ref>PMID:35388691</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eht" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Guskov A]]	+	[[Category: Levansucrase]]
-	[[Category: Hou XD]]	+	[[Category: Guskov, A]]
-	[[Category: Mu WM]]	+	[[Category: Hou, X D]]
-	[[Category: Pijning T]]	+	[[Category: Mu, W M]]
-	[[Category: Rao YJ]]	+	[[Category: Pijning, T]]
-	[[Category: Xu W]]	+	[[Category: Rao, Y J]]
		+	[[Category: Xu, W]]
		+	[[Category: Fructosyltransferase]]
		+	[[Category: Levan synthesis]]
		+	[[Category: Transferase]]

OCA at 02:48, 21 April 2022

2022-04-21T02:48:56Z

←Older revision		Revision as of 02:48, 21 April 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7eht ~~is ON HOLD until May 05 2023~~	+	==Levansucrase from Brenneria sp. EniD 312==
-		+	<StructureSection load='7eht' size='340' side='right'caption='[[7eht]]' scene=''>
-	~~Authors~~: ~~Xu, W~~.~~, Hou, X~~.D., ~~Rao, Y~~.J., ~~Pijning~~, T., ~~Guskov, A~~., ~~Mu, W~~.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHT FirstGlance]. <br>
-	~~Description: Levansucrase from Brenneria sp. EniD 312~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eht OCA], [https://pdbe.org/7eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eht RCSB], [https://www.ebi.ac.uk/pdbsum/7eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eht ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Mu, W.M~~]]	+	__TOC__
-	[[Category: ~~Guskov, A~~]]	+	</StructureSection>
-	[[Category: ~~Hou, X.D~~]]	+	[[Category: Large Structures]]
-	[[Category: Pijning, T]]	+	[[Category: Guskov A]]
-	[[Category: Rao~~, Y.J~~]]	+	[[Category: Hou XD]]
-	[[Category: Xu, W]]	+	[[Category: Mu WM]]
		+	[[Category: Pijning T]]
		+	[[Category: Rao YJ]]
		+	[[Category: Xu W]]

OCA at 04:45, 9 March 2022

2022-03-09T04:45:05Z

←Older revision		Revision as of 04:45, 9 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eht is ON HOLD until ~~Paper Publication~~	+	The entry 7eht is ON HOLD until May 05 2023

	Authors: Xu, W., Hou, X.D., Rao, Y.J., Pijning, T., Guskov, A., Mu, W.M.		Authors: Xu, W., Hou, X.D., Rao, Y.J., Pijning, T., Guskov, A., Mu, W.M.
Line 7:		Line 7:
	Description: Levansucrase from Brenneria sp. EniD 312		Description: Levansucrase from Brenneria sp. EniD 312
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Pijning, T]]
	[[Category: Mu, W.M]]		[[Category: Mu, W.M]]
	[[Category: Guskov, A]]		[[Category: Guskov, A]]
	[[Category: Hou, X.D]]		[[Category: Hou, X.D]]
		+	[[Category: Pijning, T]]
	[[Category: Rao, Y.J]]		[[Category: Rao, Y.J]]
	[[Category: Xu, W]]		[[Category: Xu, W]]

OCA at 04:10, 28 April 2021

2021-04-28T04:10:58Z

←Older revision		Revision as of 04:10, 28 April 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eht is ON HOLD	+	The entry 7eht is ON HOLD until Paper Publication

	Authors: Xu, W., Hou, X.D., Rao, Y.J., Pijning, T., Guskov, A., Mu, W.M.		Authors: Xu, W., Hou, X.D., Rao, Y.J., Pijning, T., Guskov, A., Mu, W.M.

OCA: Protected "7eht" [edit=sysop:move=sysop]

2021-04-07T04:31:20Z

Protected "7eht" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:31, 7 April 2021

OCA: New page: '''Unreleased structure''' The entry 7eht is ON HOLD Authors: Xu, W., Hou, X.D., Rao, Y.J., Pijning, T., Guskov, A., Mu, W.M. Description: Levansucrase from Brenneria sp. EniD 312 [[Ca...

2021-04-07T04:31:20Z

New page: '''Unreleased structure''' The entry 7eht is ON HOLD Authors: Xu, W., Hou, X.D., Rao, Y.J., Pijning, T., Guskov, A., Mu, W.M. Description: Levansucrase from Brenneria sp. EniD 312 [[Ca...

New page

'''Unreleased structure'''

The entry 7eht is ON HOLD

Authors: Xu, W., Hou, X.D., Rao, Y.J., Pijning, T., Guskov, A., Mu, W.M.

Description: Levansucrase from Brenneria sp. EniD 312
[[Category: Unreleased Structures]]
[[Category: Pijning, T]]
[[Category: Mu, W.M]]
[[Category: Guskov, A]]
[[Category: Hou, X.D]]
[[Category: Rao, Y.J]]
[[Category: Xu, W]]