OCA at 16:59, 29 November 2023

2023-11-29T16:59:33Z

←Older revision		Revision as of 16:59, 29 November 2023
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	==Crystal structure of exopolyphosphatase (PPX) from Porphyromonas gingivalis in complex with sulfate and magnesium ions==		==Crystal structure of exopolyphosphatase (PPX) from Porphyromonas gingivalis in complex with sulfate and magnesium ions==
-	<StructureSection load='7epq' size='340' side='right'caption='[[7epq]]' scene=''>	+	<StructureSection load='7epq' size='340' side='right'caption='[[7epq]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EPQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7epq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Porphyromonas_gingivalis_ATCC_33277 Porphyromonas gingivalis ATCC 33277]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EPQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7epq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7epq OCA], [https://pdbe.org/7epq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7epq RCSB], [https://www.ebi.ac.uk/pdbsum/7epq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7epq ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7epq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7epq OCA], [https://pdbe.org/7epq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7epq RCSB], [https://www.ebi.ac.uk/pdbsum/7epq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7epq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B2RHQ2_PORG3 B2RHQ2_PORG3]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The enzymes exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GppA) play important roles in the bacterial stringent response. PPX degrades inorganic polyphosphate (polyP), a polymer composed of a few to hundreds of phosphate residues supporting cell survival in the stationary phase. The crystal structure of PPX from Porphyromonas gingivalis (PgPPX) in complex with catalytic magnesium ions and several sulfate ions was solved. PgPPX contained two domains and represented a "closed" configuration. Four sulfate ions forming a linear dispersed chain were observed in the aqueduct of the PPX dimer, which the long polyP chain most likely occupied. The side chain of R255 stretched into the cavity where polyP could be located, obstructing the entrance of larger substrates such as NTP and NDP. This study provided the first view into the structure of the PPX/GppA homolog in complex with magnesium ions and substrate analogs and explained how PgPPX implemented its functionality.
		+
		+	The structure of exopolyphosphatase (PPX) from Porphyromonas gingivalis in complex with substrate analogs and magnesium ions reveals the basis for polyphosphate processivity.,Zhang A, Lu Z, Xu Y, Qi T, Li W, Zhang L, Cui Z J Struct Biol. 2021 Sep;213(3):107767. doi: 10.1016/j.jsb.2021.107767. Epub 2021 , Jun 30. PMID:34214602<ref>PMID:34214602</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7epq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Porphyromonas gingivalis ATCC 33277]]
	[[Category: Zhang A]]		[[Category: Zhang A]]

OCA at 06:16, 12 May 2022

2022-05-12T06:16:49Z

←Older revision		Revision as of 06:16, 12 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7epq ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of exopolyphosphatase (PPX) from Porphyromonas gingivalis in complex with sulfate and magnesium ions==
-		+	<StructureSection load='7epq' size='340' side='right'caption='[[7epq]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EPQ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7epq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7epq OCA], [https://pdbe.org/7epq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7epq RCSB], [https://www.ebi.ac.uk/pdbsum/7epq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7epq ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Zhang A]]

OCA at 11:37, 29 December 2021

2021-12-29T11:37:09Z

←Older revision		Revision as of 11:37, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7epq is ON HOLD	+	The entry 7epq is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7epq" [edit=sysop:move=sysop]

2021-05-05T07:19:38Z

Protected "7epq" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:19, 5 May 2021

OCA: New page: '''Unreleased structure''' The entry 7epq is ON HOLD Authors: Description: Category: Unreleased Structures

2021-05-05T07:19:37Z

New page: '''Unreleased structure''' The entry 7epq is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7epq is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7epq - Revision history

OCA at 16:59, 29 November 2023

OCA at 06:16, 12 May 2022

OCA at 11:37, 29 December 2021

OCA: Protected "7epq" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7epq is ON HOLD Authors: Description: Category: Unreleased Structures