OCA at 17:04, 29 November 2023

2023-11-29T17:04:37Z

←Older revision		Revision as of 17:04, 29 November 2023
Line 1:		Line 1:

-	====	+	==Crystal structure of the Lon-like protease MtaLonC with D582A mutation in complex with substrate polypeptide==
-	<StructureSection load='7euy' size='340' side='right'caption='[[7euy]]' scene=''>	+	<StructureSection load='7euy' size='340' side='right'caption='[[7euy]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7euy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Meiothermus_taiwanensis Meiothermus taiwanensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7euy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7euy OCA], [https://pdbe.org/7euy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7euy RCSB], [https://www.ebi.ac.uk/pdbsum/7euy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7euy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7euy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7euy OCA], [https://pdbe.org/7euy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7euy RCSB], [https://www.ebi.ac.uk/pdbsum/7euy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7euy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C9DRU9_9DEIN C9DRU9_9DEIN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Lon protease is the prototype of a family of proteolytic machines with adenosine triphosphatase modules built into a substrate degradation chamber. Lon is known to degrade protein substrates in a processive fashion, cutting a protein chain processively into small peptides before commencing cleavages of another protein chain. Here, we present structural and biochemical evidence demonstrating that processive substrate degradation occurs at each of the six proteolytic active sites of Lon, which forms a deep groove that partially encloses the substrate polypeptide chain by accommodating only the unprimed residues and permits processive cleavage in the C-to-N direction. We identify a universally conserved acidic residue at the exit side of the binding groove indispensable for the proteolytic activity. This noncatalytic residue likely promotes processive proteolysis by carboxyl-carboxylate interactions with cleaved intermediates. Together, these results uncover a previously unrecognized mechanism for processive substrate degradation by the Lon protease.
		+
		+	Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex.,Li S, Hsieh KY, Kuo CI, Su SC, Huang KF, Zhang K, Chang CI Sci Adv. 2021 Nov 12;7(46):eabj9537. doi: 10.1126/sciadv.abj9537. Epub 2021 Nov , 10. PMID:34757797<ref>PMID:34757797</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7euy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Meiothermus taiwanensis]]
		+	[[Category: Chang CI]]
		+	[[Category: Hsieh KY]]
		+	[[Category: Huang KF]]
		+	[[Category: Kuo CI]]
		+	[[Category: Su SC]]

OCA at 12:35, 24 November 2021

2021-11-24T12:35:07Z

←Older revision		Revision as of 12:35, 24 November 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7euy ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7euy' size='340' side='right'caption='[[7euy]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7euy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7euy OCA], [https://pdbe.org/7euy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7euy RCSB], [https://www.ebi.ac.uk/pdbsum/7euy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7euy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 06:02, 25 June 2021

2021-06-25T06:02:43Z

←Older revision		Revision as of 06:02, 25 June 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7euy is ON HOLD	+	The entry 7euy is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7euy" [edit=sysop:move=sysop]

2021-05-26T07:52:06Z

Protected "7euy" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:52, 26 May 2021

OCA: New page: '''Unreleased structure''' The entry 7euy is ON HOLD Authors: Description: Category: Unreleased Structures

2021-05-26T07:52:05Z

New page: '''Unreleased structure''' The entry 7euy is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7euy is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7euy - Revision history

OCA at 17:04, 29 November 2023

OCA at 12:35, 24 November 2021

OCA at 06:02, 25 June 2021

OCA: Protected "7euy" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7euy is ON HOLD Authors: Description: Category: Unreleased Structures