7eys - Revision history

OCA at 17:06, 29 November 2023

2023-11-29T17:06:33Z

←Older revision		Revision as of 17:06, 29 November 2023
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	==Complex structure of SptF with Fe, alpha-ketoglutarate, and andiconin D==		==Complex structure of SptF with Fe, alpha-ketoglutarate, and andiconin D==
-	<StructureSection load='7eys' size='340' side='right'caption='[[7eys]]' scene=''>	+	<StructureSection load='7eys' size='340' side='right'caption='[[7eys]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eys]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_sp. Aspergillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eys OCA], [https://pdbe.org/7eys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eys RCSB], [https://www.ebi.ac.uk/pdbsum/7eys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eys ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0CC:Andiconin+D'>0CC</scene>, <scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eys OCA], [https://pdbe.org/7eys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eys RCSB], [https://www.ebi.ac.uk/pdbsum/7eys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eys ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6J4CX17_9EURO A0A6J4CX17_9EURO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Non-heme iron and alpha-ketoglutarate-dependent (Fe/alphaKG) oxygenases catalyze various oxidative biotransformations. Due to their catalytic flexibility and high efficiency, Fe/alphaKG oxygenases have attracted keen attention for their application as biocatalysts. Here, we report the biochemical and structural characterizations of the unusually promiscuous and catalytically versatile Fe/alphaKG oxygenase SptF, involved in the biosynthesis of fungal meroterpenoid emervaridones. The in vitro analysis revealed that SptF catalyzes several continuous oxidation reactions, including hydroxylation, desaturation, epoxidation, and skeletal rearrangement. SptF exhibits extremely broad substrate specificity toward various meroterpenoids, and efficiently produced unique cyclopropane-ring-fused 5/3/5/5/6/6 and 5/3/6/6/6 scaffolds from terretonins. Moreover, SptF also hydroxylates steroids, including androsterone, testosterone, and progesterone, with different regiospecificities. Crystallographic and structure-based mutagenesis studies of SptF revealed the molecular basis of the enzyme reactions, and suggested that the malleability of the loop region contributes to the remarkable substrate promiscuity. SptF exhibits great potential as a promising biocatalyst for oxidation reactions.
		+
		+	Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/alpha-ketoglutarate-dependent oxygenase SptF.,Tao H, Mori T, Chen H, Lyu S, Nonoyama A, Lee S, Abe I Nat Commun. 2022 Jan 10;13(1):95. doi: 10.1038/s41467-021-27636-3. PMID:35013177<ref>PMID:35013177</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eys" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspergillus sp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abe I]]		[[Category: Abe I]]
	[[Category: Mori T]]		[[Category: Mori T]]
	[[Category: Tao H]]		[[Category: Tao H]]

OCA at 02:51, 21 April 2022

2022-04-21T02:51:05Z

←Older revision		Revision as of 02:51, 21 April 2022
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-	'''Unreleased structure'''

-	~~The entry 7eys is ON HOLD until Paper Publication~~	+	==Complex structure of SptF with Fe, alpha-ketoglutarate, and andiconin D==
-		+	<StructureSection load='7eys' size='340' side='right'caption='[[7eys]]' scene=''>
-	~~Authors: Tao, H., Mori, T., Abe, I.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYS FirstGlance]. <br>
-	~~Description:~~ Complex structure of SptF with Fe, alpha-ketoglutarate, and andiconin D	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eys OCA], [https://pdbe.org/7eys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eys RCSB], [https://www.ebi.ac.uk/pdbsum/7eys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eys ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Abe, I]]	+	__TOC__
-	[[Category: Mori, T]]	+	</StructureSection>
-	[[Category: Tao, H]]	+	[[Category: Large Structures]]
		+	[[Category: Abe I]]
		+	[[Category: Mori T]]
		+	[[Category: Tao H]]

OCA at 06:03, 25 June 2021

2021-06-25T06:03:44Z

←Older revision		Revision as of 06:03, 25 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eys is ON HOLD	+	The entry 7eys is ON HOLD until Paper Publication

	Authors: Tao, H., Mori, T., Abe, I.		Authors: Tao, H., Mori, T., Abe, I.

OCA: Protected "7eys" [edit=sysop:move=sysop]

2021-06-09T10:59:34Z

Protected "7eys" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 9 June 2021

OCA: New page: '''Unreleased structure''' The entry 7eys is ON HOLD Authors: Tao, H., Mori, T., Abe, I. Description: Complex structure of SptF with Fe, alpha-ketoglutarate, and andiconin D [[Category...

2021-06-09T10:59:33Z

New page: '''Unreleased structure''' The entry 7eys is ON HOLD Authors: Tao, H., Mori, T., Abe, I. Description: Complex structure of SptF with Fe, alpha-ketoglutarate, and andiconin D [[Category...

New page

'''Unreleased structure'''

The entry 7eys is ON HOLD

Authors: Tao, H., Mori, T., Abe, I.

Description: Complex structure of SptF with Fe, alpha-ketoglutarate, and andiconin D
[[Category: Unreleased Structures]]
[[Category: Abe, I]]
[[Category: Mori, T]]
[[Category: Tao, H]]