7eyt - Revision history

OCA at 17:06, 29 November 2023

2023-11-29T17:06:36Z

←Older revision		Revision as of 17:06, 29 November 2023
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	==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG==		==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG==
-	<StructureSection load='7eyt' size='340' side='right'caption='[[7eyt]]' scene=''>	+	<StructureSection load='7eyt' size='340' side='right'caption='[[7eyt]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eyt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_sp. Aspergillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyt OCA], [https://pdbe.org/7eyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyt RCSB], [https://www.ebi.ac.uk/pdbsum/7eyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyt ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0I3:Andilesin+C'>0I3</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=OGA:N-OXALYLGLYCINE'>OGA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyt OCA], [https://pdbe.org/7eyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyt RCSB], [https://www.ebi.ac.uk/pdbsum/7eyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyt ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6J4CX17_9EURO A0A6J4CX17_9EURO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Non-heme iron and alpha-ketoglutarate-dependent (Fe/alphaKG) oxygenases catalyze various oxidative biotransformations. Due to their catalytic flexibility and high efficiency, Fe/alphaKG oxygenases have attracted keen attention for their application as biocatalysts. Here, we report the biochemical and structural characterizations of the unusually promiscuous and catalytically versatile Fe/alphaKG oxygenase SptF, involved in the biosynthesis of fungal meroterpenoid emervaridones. The in vitro analysis revealed that SptF catalyzes several continuous oxidation reactions, including hydroxylation, desaturation, epoxidation, and skeletal rearrangement. SptF exhibits extremely broad substrate specificity toward various meroterpenoids, and efficiently produced unique cyclopropane-ring-fused 5/3/5/5/6/6 and 5/3/6/6/6 scaffolds from terretonins. Moreover, SptF also hydroxylates steroids, including androsterone, testosterone, and progesterone, with different regiospecificities. Crystallographic and structure-based mutagenesis studies of SptF revealed the molecular basis of the enzyme reactions, and suggested that the malleability of the loop region contributes to the remarkable substrate promiscuity. SptF exhibits great potential as a promising biocatalyst for oxidation reactions.
		+
		+	Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/alpha-ketoglutarate-dependent oxygenase SptF.,Tao H, Mori T, Chen H, Lyu S, Nonoyama A, Lee S, Abe I Nat Commun. 2022 Jan 10;13(1):95. doi: 10.1038/s41467-021-27636-3. PMID:35013177<ref>PMID:35013177</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eyt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dioxygenase 3D structures\|Dioxygenase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspergillus sp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abe I]]		[[Category: Abe I]]
	[[Category: Mori T]]		[[Category: Mori T]]
	[[Category: Tao H]]		[[Category: Tao H]]

OCA at 02:51, 21 April 2022

2022-04-21T02:51:14Z

←Older revision		Revision as of 02:51, 21 April 2022
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-	'''Unreleased structure'''

-	~~The entry 7eyt is ON HOLD until Paper Publication~~	+	==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG==
-		+	<StructureSection load='7eyt' size='340' side='right'caption='[[7eyt]]' scene=''>
-	~~Authors: Tao, H., Mori, T., Abe, I.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYT FirstGlance]. <br>
-	~~Description:~~ Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyt OCA], [https://pdbe.org/7eyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyt RCSB], [https://www.ebi.ac.uk/pdbsum/7eyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyt ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Abe, I]]	+	__TOC__
-	[[Category: Mori, T]]	+	</StructureSection>
-	[[Category: Tao, H]]	+	[[Category: Large Structures]]
		+	[[Category: Abe I]]
		+	[[Category: Mori T]]
		+	[[Category: Tao H]]

OCA at 04:02, 6 October 2021

2021-10-06T04:02:01Z

←Older revision		Revision as of 04:02, 6 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eyt is ON HOLD	+	The entry 7eyt is ON HOLD until Paper Publication

	Authors: Tao, H., Mori, T., Abe, I.		Authors: Tao, H., Mori, T., Abe, I.

OCA at 07:20, 8 September 2021

2021-09-08T07:20:13Z

←Older revision		Revision as of 07:20, 8 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eyt is ON HOLD ~~until Paper Publication~~	+	The entry 7eyt is ON HOLD

	Authors: Tao, H., Mori, T., Abe, I.		Authors: Tao, H., Mori, T., Abe, I.

OCA at 06:03, 25 June 2021

2021-06-25T06:03:47Z

←Older revision		Revision as of 06:03, 25 June 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eyt is ON HOLD	+	The entry 7eyt is ON HOLD until Paper Publication

	Authors: Tao, H., Mori, T., Abe, I.		Authors: Tao, H., Mori, T., Abe, I.

OCA: Protected "7eyt" [edit=sysop:move=sysop]

2021-06-09T10:59:37Z

Protected "7eyt" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 9 June 2021

OCA: New page: '''Unreleased structure''' The entry 7eyt is ON HOLD Authors: Tao, H., Mori, T., Abe, I. Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG [[...

2021-06-09T10:59:36Z

New page: '''Unreleased structure''' The entry 7eyt is ON HOLD Authors: Tao, H., Mori, T., Abe, I. Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG [[...

New page

'''Unreleased structure'''

The entry 7eyt is ON HOLD

Authors: Tao, H., Mori, T., Abe, I.

Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG
[[Category: Unreleased Structures]]
[[Category: Abe, I]]
[[Category: Mori, T]]
[[Category: Tao, H]]