OCA at 17:08, 29 November 2023

2023-11-29T17:08:09Z

←Older revision		Revision as of 17:08, 29 November 2023
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	==L-lactate oxidase with L-lactate==		==L-lactate oxidase with L-lactate==
-	<StructureSection load='7f20' size='340' side='right'caption='[[7f20]]' scene=''>	+	<StructureSection load='7f20' size='340' side='right'caption='[[7f20]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F20 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7f20]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aerococcus_viridans Aerococcus viridans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F20 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f20 OCA], [https://pdbe.org/7f20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f20 RCSB], [https://www.ebi.ac.uk/pdbsum/7f20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f20 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2OP:(2S)-2-HYDROXYPROPANOIC+ACID'>2OP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f20 OCA], [https://pdbe.org/7f20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f20 RCSB], [https://www.ebi.ac.uk/pdbsum/7f20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f20 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LOX_AERVM LOX_AERVM] Catalyzes the oxidation of (S)-lactate (L-lactate) to pyruvate, with a reduction of O2 to H2O2 (Ref.1, PubMed:27302031, PubMed:25423902, PubMed:2818595, PubMed:8589073, PubMed:26260739). Cannot oxidize D-lactate, glycolate, and D,L-2-hydroxybutanoate (PubMed:2818595). May be involved in the utilization of L-lactate as an energy source for growth (By similarity).[UniProtKB:O33655]<ref>PMID:25423902</ref> <ref>PMID:26260739</ref> <ref>PMID:27302031</ref> <ref>PMID:2818595</ref> <ref>PMID:8589073</ref> [UniProtKB:O33655]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of l-lactate oxidase in complex with l-lactate was solved at a 1.33 A resolution. The electron density of the bound l-lactate was clearly shown and comparisons of the free form and substrate bound complexes demonstrated that l-lactate was bound to the FMN and an additional active site within the enzyme complex. l-lactate interacted with the related side chains, which play an important role in enzymatic catalysis and especially the coupled movement of H265 and D174, which may be essential to activity. These observations not only reveal the enzymatic mechanism for l-lactate binding but also demonstrate the dynamic motion of these enzyme structures in response to substrate binding and enzymatic reaction progression.
		+
		+	Dynamic interactions in the l-lactate oxidase active site facilitate substrate binding at pH4.5.,Furubayashi N, Inaka K, Kamo M, Umena Y, Matsuoka T, Morimoto Y Biochem Biophys Res Commun. 2021 Sep 3;568:131-135. doi:, 10.1016/j.bbrc.2021.06.078. Epub 2021 Jun 30. PMID:34214876<ref>PMID:34214876</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7f20" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aerococcus viridans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Inaka K]]		[[Category: Inaka K]]
	[[Category: Morimoto Y]]		[[Category: Morimoto Y]]

OCA at 11:16, 23 March 2022

2022-03-23T11:16:44Z

←Older revision		Revision as of 11:16, 23 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7f20 ~~is ON HOLD until Paper Publication~~	+	==L-lactate oxidase with L-lactate==
-		+	<StructureSection load='7f20' size='340' side='right'caption='[[7f20]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F20 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f20 OCA], [https://pdbe.org/7f20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f20 RCSB], [https://www.ebi.ac.uk/pdbsum/7f20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f20 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Inaka K]]
		+	[[Category: Morimoto Y]]

OCA at 11:38, 29 December 2021

2021-12-29T11:38:58Z

←Older revision		Revision as of 11:38, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7f20 is ON HOLD	+	The entry 7f20 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7f20" [edit=sysop:move=sysop]

2021-06-17T13:33:50Z

Protected "7f20" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:33, 17 June 2021

OCA: New page: '''Unreleased structure''' The entry 7f20 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-06-17T13:33:49Z

New page: '''Unreleased structure''' The entry 7f20 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7f20 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7f20 - Revision history

OCA at 17:08, 29 November 2023

OCA at 11:16, 23 March 2022

OCA at 11:38, 29 December 2021

OCA: Protected "7f20" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7f20 is ON HOLD Authors: Description: Category: Unreleased Structures