7f28 - Revision history

OCA at 17:08, 29 November 2023

2023-11-29T17:08:17Z

←Older revision		Revision as of 17:08, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7f28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii_NCGM_237 Acinetobacter baumannii NCGM 237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F28 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7f28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii_NCGM_237 Acinetobacter baumannii NCGM 237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F28 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f28 OCA], [https://pdbe.org/7f28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f28 RCSB], [https://www.ebi.ac.uk/pdbsum/7f28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f28 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.877Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f28 OCA], [https://pdbe.org/7f28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f28 RCSB], [https://www.ebi.ac.uk/pdbsum/7f28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f28 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:13, 28 December 2022

2022-12-28T09:13:13Z

←Older revision		Revision as of 09:13, 28 December 2022
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-	====	+	==Crystal structure of a bacterial ketosynthase==
-	<StructureSection load='7f28' size='340' side='right'caption='[[7f28]]' scene=''>	+	<StructureSection load='7f28' size='340' side='right'caption='[[7f28]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7f28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii_NCGM_237 Acinetobacter baumannii NCGM 237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F28 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f28 OCA], [https://pdbe.org/7f28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f28 RCSB], [https://www.ebi.ac.uk/pdbsum/7f28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f28 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f28 OCA], [https://pdbe.org/7f28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f28 RCSB], [https://www.ebi.ac.uk/pdbsum/7f28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f28 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0E1PUF7_ACIBA A0A0E1PUF7_ACIBA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aryl polyenes (APE) are one of the most widespread secondary metabolites among gram-negative bacteria. In Acinetobacter baumannii, strains belonging to the virulent global clone 2 (GC2) mostly contain APE biosynthesis genes; its relevance in elevated pathogenicity is of great interest. APE biosynthesis gene clusters harbor two ketosynthases (KSs): the heterodimeric KS-chain length factor complex, ApeO-ApeC, and the homodimeric ketoacyl-acyl carrier protein synthase I (FabB)-like KS, ApeR. The role of the two KSs in APE biosynthesis is unclear. We determined the crystal structures of the two KSs from a pathogenic A. baumannii strain. ApeO-ApeC and ApeR have similar cavity volumes; however, ApeR has a narrow cavity near the entrance. In vitro assay based on the absorption characteristics of polyene species indicated the generation of fully elongated polyene with only ApeO-ApeC, probably because of the funnel shaped active site cavity. However, adding ApeR to the reaction increases the throughput of APE biosynthesis. Mutagenesis at Tyr135 in the active site cavity of ApeR reduces the activity significantly, which suggests that the stacking of the aryl group between Tyr135 and Phe202 is important for substrate recognition. Therefore, the two KSs function complementarily in the generation of APE to enhance its production.
		+
		+	Structural basis of the complementary activity of two ketosynthases in aryl polyene biosynthesis.,Lee WC, Choi S, Jang A, Yeon J, Hwang E, Kim Y Sci Rep. 2021 Aug 11;11(1):16340. doi: 10.1038/s41598-021-95890-y. PMID:34381152<ref>PMID:34381152</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7f28" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acinetobacter baumannii NCGM 237]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Kim Y]]
		+	[[Category: Lee WC]]

OCA at 05:08, 15 June 2022

2022-06-15T05:08:32Z

←Older revision		Revision as of 05:08, 15 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7f28 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7f28' size='340' side='right'caption='[[7f28]]' scene=''>
-	~~Authors~~: ~~Lee, W~~.C.~~, Kim, Y~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal structure of~~ a ~~bacterial ketosynthase~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f28 OCA], [https://pdbe.org/7f28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f28 RCSB], [https://www.ebi.ac.uk/pdbsum/7f28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f28 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Lee~~, W.C]]	+	__TOC__
-	[[Category: ~~Kim, Y~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:20, 8 September 2021

2021-09-08T07:20:23Z

←Older revision		Revision as of 07:20, 8 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7f28 is ON HOLD	+	The entry 7f28 is ON HOLD until Paper Publication

	Authors: Lee, W.C., Kim, Y.		Authors: Lee, W.C., Kim, Y.

OCA: Protected "7f28" [edit=sysop:move=sysop]

2021-06-17T13:34:08Z

Protected "7f28" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:34, 17 June 2021

OCA: New page: '''Unreleased structure''' The entry 7f28 is ON HOLD Authors: Lee, W.C., Kim, Y. Description: Crystal structure of a bacterial ketosynthase Category: Unreleased Structures [[Catego...

2021-06-17T13:34:08Z

New page: '''Unreleased structure''' The entry 7f28 is ON HOLD Authors: Lee, W.C., Kim, Y. Description: Crystal structure of a bacterial ketosynthase Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 7f28 is ON HOLD

Authors: Lee, W.C., Kim, Y.

Description: Crystal structure of a bacterial ketosynthase
[[Category: Unreleased Structures]]
[[Category: Lee, W.C]]
[[Category: Kim, Y]]