OCA at 17:15, 29 November 2023

2023-11-29T17:15:22Z

←Older revision		Revision as of 17:15, 29 November 2023
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	==Osmium-bound human serum transferrin==		==Osmium-bound human serum transferrin==
-	<StructureSection load='7ffu' size='340' side='right'caption='[[7ffu]]' scene=''>	+	<StructureSection load='7ffu' size='340' side='right'caption='[[7ffu]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FFU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ffu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FFU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ffu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ffu OCA], [https://pdbe.org/7ffu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ffu RCSB], [https://www.ebi.ac.uk/pdbsum/7ffu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ffu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.601Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=OS:OSMIUM+ION'>OS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ffu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ffu OCA], [https://pdbe.org/7ffu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ffu RCSB], [https://www.ebi.ac.uk/pdbsum/7ffu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ffu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/TRFE_HUMAN TRFE_HUMAN] Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:[https://omim.org/entry/209300 209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.<ref>PMID:11110675</ref> <ref>PMID:15466165</ref>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRFE_HUMAN TRFE_HUMAN] Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Being identified with less toxic and generally showing selective effects for solid tumor metastases, ruthenium and osmium compounds are promising drug candidates for clinical uses. Human serum proteins, such as albumin and transferrin, play vital roles in the transportation and accumulation of ruthenium and osmium agents into target tissues. However, the molecular mechanism of how transferrin transport ruthenium and their osmium analogues at atomic level remains obscure. In this study, we uncovered that the cellular uptake of Os(3+) or Ru(3+) are not competed by Fe(3+). To unveil the molecular mechanism behind the phenomena, we report the first crystal structures of human serum transferrin (hTF) in complex with ruthenium and osmium compounds bound to the non-conserved residues on the surface of hTF without altering its overall conformation. As for Ru(3+) and Os(3+), these binding sites by descending affinity are: His14/His289, His349-350 ~ His578/Arg581. Ruthenium drugs and their osmium analogues preferentially bind to His14/His289 with bipyridine or imidazole ligands leaving. These binding sites on hTF surface are also available in human lactoferrin and some transferrin family member of other species. The presence of these binding sites makes the cellular uptake of Ru(3+) and Os(3+) less affected by Fe(3+), compare to Zr(4+) or Hf(4+). Collectively, these findings are critical for our understanding of the role of serum transferrin in cellular delivery of ruthenium and osmium anticancer agents.
		+
		+	Binding of ruthenium and osmium at noniron sites of transferrin accounts for their iron-independent cellular uptake.,Wang M, Wang H, Xu X, Lai TP, Zhou Y, Hao Q, Li H, Sun H J Inorg Biochem. 2022 Jun 2;234:111885. doi: 10.1016/j.jinorgbio.2022.111885. PMID:35690040<ref>PMID:35690040</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ffu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sun H]]		[[Category: Sun H]]
	[[Category: Wang M]]		[[Category: Wang M]]

OCA at 09:43, 22 June 2022

2022-06-22T09:43:24Z

←Older revision		Revision as of 09:43, 22 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ffu ~~is ON HOLD until Paper Publication~~	+	==Osmium-bound human serum transferrin==
-		+	<StructureSection load='7ffu' size='340' side='right'caption='[[7ffu]]' scene=''>
-	~~Authors~~: ~~Wang~~, M., ~~Sun~~, H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FFU FirstGlance]. <br>
-	~~Description~~: ~~Osmium~~-~~bound human serum transferrin~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ffu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ffu OCA], [https://pdbe.org/7ffu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ffu RCSB], [https://www.ebi.ac.uk/pdbsum/7ffu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ffu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Wang, M~~]]	+	__TOC__
-	[[Category: ~~Sun, H~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Sun H]]
		+	[[Category: Wang M]]

OCA at 05:06, 25 August 2021

2021-08-25T05:06:07Z

←Older revision		Revision as of 05:06, 25 August 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ffu is ON HOLD	+	The entry 7ffu is ON HOLD until Paper Publication

	Authors: Wang, M., Sun, H.		Authors: Wang, M., Sun, H.

OCA: Protected "7ffu" [edit=sysop:move=sysop]

2021-07-28T09:23:31Z

Protected "7ffu" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:23, 28 July 2021

OCA: New page: '''Unreleased structure''' The entry 7ffu is ON HOLD Authors: Wang, M., Sun, H. Description: Osmium-bound human serum transferrin Category: Unreleased Structures [[Category: Wang, ...

2021-07-28T09:23:30Z

New page: '''Unreleased structure''' The entry 7ffu is ON HOLD Authors: Wang, M., Sun, H. Description: Osmium-bound human serum transferrin Category: Unreleased Structures [[Category: Wang, ...

New page

'''Unreleased structure'''

The entry 7ffu is ON HOLD

Authors: Wang, M., Sun, H.

Description: Osmium-bound human serum transferrin
[[Category: Unreleased Structures]]
[[Category: Wang, M]]
[[Category: Sun, H]]

7ffu - Revision history

OCA at 17:15, 29 November 2023

OCA at 09:43, 22 June 2022

OCA at 05:06, 25 August 2021

OCA: Protected "7ffu" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ffu is ON HOLD Authors: Wang, M., Sun, H. Description: Osmium-bound human serum transferrin Category: Unreleased Structures [[Category: Wang, ...