OCA at 04:07, 8 September 2022

2022-09-08T04:07:12Z

←Older revision		Revision as of 04:07, 8 September 2022
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-	====	+	==Cryo-EM structure of a membrane protein(LL)==
-	<StructureSection load='7fje' size='340' side='right'caption='[[7fje]]' scene=''>	+	<StructureSection load='7fje' size='340' side='right'caption='[[7fje]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7fje]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FJE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fje OCA], [https://pdbe.org/7fje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fje RCSB], [https://www.ebi.ac.uk/pdbsum/7fje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fje ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fje OCA], [https://pdbe.org/7fje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fje RCSB], [https://www.ebi.ac.uk/pdbsum/7fje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fje ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[https://www.uniprot.org/uniprot/CD3Z_HUMAN CD3Z_HUMAN]] Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:[https://omim.org/entry/610163 610163]]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.<ref>PMID:16672702</ref>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/CD3Z_HUMAN CD3Z_HUMAN]] Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cholesterol molecules specifically bind to the resting alphabetaTCR to inhibit cytoplasmic CD3zeta ITAM phosphorylation through sequestering the TCR-CD3 complex in an inactive conformation. The mechanisms of cholesterol-mediated inhibition of TCR-CD3 and its activation remain unclear. Here, we present cryoelectron microscopy structures of cholesterol- and cholesterol sulfate (CS)-inhibited TCR-CD3 complexes and an auto-active TCR-CD3 variant. The structures reveal that cholesterol molecules act like a latch to lock CD3zeta into an inactive conformation in the membrane. Mutations impairing binding of cholesterol molecules to the tunnel result in the movement of the proximal C terminus of the CD3zeta transmembrane helix, thereby activating the TCR-CD3 complex in human cells. Together, our data reveal the structural basis of TCR inhibition by cholesterol, illustrate how the cholesterol-binding tunnel is allosterically coupled to TCR triggering, and lay a foundation for the development of immunotherapies through directly targeting the TCR-CD3 complex.
		+
		+	Cholesterol inhibits TCR signaling by directly restricting TCR-CD3 core tunnel motility.,Chen Y, Zhu Y, Li X, Gao W, Zhen Z, Dong, Huang B, Ma Z, Zhang A, Song X, Ma Y, Guo C, Zhang F, Huang Z Mol Cell. 2022 Apr 7;82(7):1278-1287.e5. doi: 10.1016/j.molcel.2022.02.017. Epub , 2022 Mar 9. PMID:35271814<ref>PMID:35271814</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7fje" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Chen Y]]
		+	[[Category: Gao W]]
		+	[[Category: Guo C]]
		+	[[Category: Huang Z]]
		+	[[Category: Zhang A]]
		+	[[Category: Zhu Y]]

OCA at 18:00, 27 July 2022

2022-07-27T18:00:16Z

←Older revision		Revision as of 18:00, 27 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7fje ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7fje' size='340' side='right'caption='[[7fje]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fje OCA], [https://pdbe.org/7fje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fje RCSB], [https://www.ebi.ac.uk/pdbsum/7fje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fje ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 11:45, 29 December 2021

2021-12-29T11:45:17Z

←Older revision		Revision as of 11:45, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7fje is ON HOLD	+	The entry 7fje is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7fje" [edit=sysop:move=sysop]

2021-08-11T04:59:30Z

Protected "7fje" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:59, 11 August 2021

OCA: New page: '''Unreleased structure''' The entry 7fje is ON HOLD Authors: Description: Category: Unreleased Structures

2021-08-11T04:59:29Z

New page: '''Unreleased structure''' The entry 7fje is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7fje is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7fje - Revision history

OCA at 04:07, 8 September 2022

OCA at 18:00, 27 July 2022

OCA at 11:45, 29 December 2021

OCA: Protected "7fje" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7fje is ON HOLD Authors: Description: Category: Unreleased Structures