7jqz - Revision history

OCA at 15:09, 18 October 2023

2023-10-18T15:09:47Z

←Older revision		Revision as of 15:09, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='7jqz' size='340' side='right'caption='[[7jqz]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='7jqz' size='340' side='right'caption='[[7jqz]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7jqz]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Burcc Burcc~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JQZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jqz]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cenocepacia_MC0-3 Burkholderia cenocepacia MC0-3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JQZ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[7jqx\|7jqx]], [[7jqy\|7jqy]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">Bcenmc03_3580 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=406425 BURCC])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jqz OCA], [https://pdbe.org/7jqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jqz RCSB], [https://www.ebi.ac.uk/pdbsum/7jqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jqz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jqz OCA], [https://pdbe.org/7jqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jqz RCSB], [https://www.ebi.ac.uk/pdbsum/7jqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jqz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B1K378_BURCC B1K378_BURCC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Burcc~~]]	+	[[Category: Burkholderia cenocepacia MC0-3]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Madden~~, D R~~]]	+	[[Category: Madden DR]]
-	[[Category: Taher~~, N M]]~~	+	[[Category: Taher NM]]
-	~~[[Category: Alpha/beta hydrolase fold]]~~	+
-	~~[[Category: Decamer]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 10:48, 28 July 2021

2021-07-28T10:48:47Z

←Older revision		Revision as of 10:48, 28 July 2021
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	==Crystal structure of Cfl2 wild-type from Burkholderia cenocepacia==		==Crystal structure of Cfl2 wild-type from Burkholderia cenocepacia==
-	<StructureSection load='7jqz' size='340' side='right'caption='[[7jqz]]' scene=''>	+	<StructureSection load='7jqz' size='340' side='right'caption='[[7jqz]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JQZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jqz]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Burcc Burcc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JQZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jqz OCA], [https://pdbe.org/7jqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jqz RCSB], [https://www.ebi.ac.uk/pdbsum/7jqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jqz ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7jqx\|7jqx]], [[7jqy\|7jqy]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bcenmc03_3580 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=406425 BURCC])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jqz OCA], [https://pdbe.org/7jqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jqz RCSB], [https://www.ebi.ac.uk/pdbsum/7jqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jqz ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Epoxide hydrolases catalyze the conversion of epoxides to vicinal diols in a range of cellular processes such as signaling, detoxification, and virulence. These enzymes typically utilize a pair of tyrosine residues to orient the substrate epoxide ring in the active site and stabilize the hydrolysis intermediate. A new subclass of epoxide hydrolases that utilize a histidine in place of one of the tyrosines was established with the discovery of the CFTR Inhibitory Factor (Cif) from Pseudomonas aeruginosa. Although the presence of such Cif-like epoxide hydrolases was predicted in other opportunistic pathogens based on sequence analyses, only Cif and its homolog aCif from Acinetobacter nosocomialis have been characterized. Here we report the biochemical and structural characteristics of Cfl1 and Cfl2, two Cif-like epoxide hydrolases from Burkholderia cenocepacia. Cfl1 is able to hydrolyze xenobiotic as well as biological epoxides that might be encountered in the environment or during infection. In contrast, Cfl2 shows very low activity against a diverse set of epoxides. The crystal structures of the two proteins reveal quaternary structures that build on the well-known dimeric assembly of the alpha/beta hydrolase domain, but broaden our understanding of the structural diversity encoded in novel oligomer interfaces. Analysis of the interfaces reveals both similarities and key differences in sequence conservation between the two assemblies, and between the canonical dimer and the novel oligomer interfaces of each assembly. Finally, we discuss the effects of these higher-order assemblies on the intra-monomer flexibility of Cfl1 and Cfl2 and their possible roles in regulating enzymatic activity.
		+
		+	Biochemical and structural characterization of two cif-like epoxide hydrolases from Burkholderia cenocepacia.,Taher NM, Hvorecny KL, Burke CM, Gilman MSA, Heussler GE, Adolf-Bryfogle J, Bahl CD, O'Toole GA, Madden DR Curr Res Struct Biol. 2021 Feb 21;3:72-84. doi: 10.1016/j.crstbi.2021.02.002., eCollection 2021. PMID:34235487<ref>PMID:34235487</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jqz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Burcc]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Madden DR]]	+	[[Category: Madden, D R]]
-	[[Category: Taher NM]]	+	[[Category: Taher, N M]]
		+	[[Category: Alpha/beta hydrolase fold]]
		+	[[Category: Decamer]]
		+	[[Category: Hydrolase]]

OCA at 06:47, 17 March 2021

2021-03-17T06:47:46Z

←Older revision		Revision as of 06:47, 17 March 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7jqz ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of Cfl2 wild-type from Burkholderia cenocepacia==
-		+	<StructureSection load='7jqz' size='340' side='right'caption='[[7jqz]]' scene=''>
-	~~Authors~~: ~~Taher~~, N.M., ~~Madden~~, D.R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JQZ FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of Cfl2 wild~~-~~type from Burkholderia cenocepacia~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jqz OCA], [https://pdbe.org/7jqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jqz RCSB], [https://www.ebi.ac.uk/pdbsum/7jqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jqz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Taher, N.M~~]]	+	__TOC__
-	[[Category: ~~Madden, D.R~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Madden DR]]
		+	[[Category: Taher NM]]

OCA at 07:00, 16 September 2020

2020-09-16T07:00:25Z

←Older revision		Revision as of 07:00, 16 September 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7jqz is ON HOLD	+	The entry 7jqz is ON HOLD until Paper Publication

	Authors: Taher, N.M., Madden, D.R.		Authors: Taher, N.M., Madden, D.R.

OCA: Protected "7jqz" [edit=sysop:move=sysop]

2020-08-19T04:56:00Z

Protected "7jqz" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:56, 19 August 2020

OCA: New page: '''Unreleased structure''' The entry 7jqz is ON HOLD Authors: Taher, N.M., Madden, D.R. Description: Crystal structure of Cfl2 wild-type from Burkholderia cenocepacia [[Category: Unrel...

2020-08-19T04:55:58Z

New page: '''Unreleased structure''' The entry 7jqz is ON HOLD Authors: Taher, N.M., Madden, D.R. Description: Crystal structure of Cfl2 wild-type from Burkholderia cenocepacia [[Category: Unrel...

New page

'''Unreleased structure'''

The entry 7jqz is ON HOLD

Authors: Taher, N.M., Madden, D.R.

Description: Crystal structure of Cfl2 wild-type from Burkholderia cenocepacia
[[Category: Unreleased Structures]]
[[Category: Taher, N.M]]
[[Category: Madden, D.R]]