7kb7 - Revision history

OCA at 15:20, 18 October 2023

2023-10-18T15:20:35Z

←Older revision		Revision as of 15:20, 18 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7kb7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KB7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7kb7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KB7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kb7 OCA], [https://pdbe.org/7kb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kb7 RCSB], [https://www.ebi.ac.uk/pdbsum/7kb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kb7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kb7 OCA], [https://pdbe.org/7kb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kb7 RCSB], [https://www.ebi.ac.uk/pdbsum/7kb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kb7 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 20:20, 16 November 2022

2022-11-16T20:20:38Z

←Older revision		Revision as of 20:20, 16 November 2022
Line 1:		Line 1:

	==THE STRUCTURE OF A SENSOR DOMAIN OF A HISTIDINE KINASE (VxrA) FROM VIBRIO CHOLERAE O1 BIOVAR ELTOR STR. N16961, N239-T240 deletion mutant==		==THE STRUCTURE OF A SENSOR DOMAIN OF A HISTIDINE KINASE (VxrA) FROM VIBRIO CHOLERAE O1 BIOVAR ELTOR STR. N16961, N239-T240 deletion mutant==
-	<StructureSection load='7kb7' size='340' side='right'caption='[[7kb7]]' scene=''>	+	<StructureSection load='7kb7' size='340' side='right'caption='[[7kb7]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KB7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7KB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kb7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KB7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7kb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kb7 OCA], [~~http~~://pdbe.org/7kb7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7kb7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7kb7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7kb7 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kb7 OCA], [https://pdbe.org/7kb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kb7 RCSB], [https://www.ebi.ac.uk/pdbsum/7kb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kb7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9KM24_VIBCH Q9KM24_VIBCH]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	VxrA and VxrB are cognate histidine kinase (HK) - response regulator (RR) pairs of a two-component signaling system (TCS) found in Vibrio cholerae, a bacterial pathogen that causes cholera. The VxrAB TCS positively regulates virulence, the Type VI Secretion System, biofilm formation, and cell wall homeostasis in V. cholerae, providing protection from environmental stresses and contributing to the transmission and virulence of the pathogen. The VxrA HK has a unique periplasmic sensor domain (SD) and, remarkably, lacks a cytoplasmic linker domain between the second transmembrane helix and the dimerization and histidine phosphotransfer (DHp) domain, indicating that this system may utilize a potentially unique signal sensing and transmission TCS mechanism. In this study, we have determined several crystal structures of VxrA-SD and its mutants. These structures reveal a novel structural fold forming an unusual beta hairpin-swapped dimer. A conformational change caused by relative rotation of the two monomers in a VxrA-SD dimer could potentially change the association of transmembrane helices and, subsequently, the pairing of cytoplasmic DHp domains. Based on the structural observation, we propose a putative scissor-like closing regulation mechanism for the VxrA HK.IMPORTANCE V. cholerae has a dynamic life cycle, which requires rapid adaptation to changing external conditions. Two-component signal transduction (TCS) systems allow V. cholerae to sense and respond to these environmental changes. The VxrAB TCS positively regulates a number of important V. cholerae phenotypes, including virulence, the Type Six Secretion System, biofilm formation, and cell wall homeostasis. Here, we provide the crystal structure of the VxrA sensor histidine kinase sensing domain and propose a mechanism for signal transduction. The cognate signal for VxrAB remains unknown, however, in this work we couple our structural analysis with functional assessments of key residues to further our understanding of this important TCS.
		+
		+	Sensor Domain of Histidine Kinase VxrA of Vibrio cholerae- A Hairpin-swapped Dimer and its Conformational Change.,Tan K, Teschler JK, Wu R, Jedrzejczak RP, Zhou M, Shuvalova LA, Endres MJ, Welk LF, Kwon K, Anderson WF, Satchell KJF, Yildiz FH, Joachimiak A J Bacteriol. 2021 Mar 22. pii: JB.00643-20. doi: 10.1128/JB.00643-20. PMID:33753465<ref>PMID:33753465</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kb7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
	[[Category: Jedrzejczak R]]		[[Category: Jedrzejczak R]]
	[[Category: Joachimiak A]]		[[Category: Joachimiak A]]
	[[Category: Tan K]]		[[Category: Tan K]]
	[[Category: Wu R]]		[[Category: Wu R]]

OCA at 05:53, 14 October 2020

2020-10-14T05:53:27Z

←Older revision		Revision as of 05:53, 14 October 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7kb7 is ON HOLD~~	+	==THE STRUCTURE OF A SENSOR DOMAIN OF A HISTIDINE KINASE (VxrA) FROM VIBRIO CHOLERAE O1 BIOVAR ELTOR STR. N16961, N239-T240 deletion mutant==
-		+	<StructureSection load='7kb7' size='340' side='right'caption='[[7kb7]]' scene=''>
-	~~Authors: Tan, K., Wu, R., Jedrzejczak, R., Joachimiak, A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KB7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KB7 FirstGlance]. <br>
-	~~Description:~~ THE STRUCTURE OF A SENSOR DOMAIN OF A HISTIDINE KINASE (VxrA) FROM VIBRIO CHOLERAE O1 BIOVAR ELTOR STR. N16961, N239-T240 deletion mutant	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kb7 OCA], [http://pdbe.org/7kb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kb7 RCSB], [http://www.ebi.ac.uk/pdbsum/7kb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kb7 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Joachimiak~~, A]]	+	__TOC__
-	[[Category: ~~Tan, K~~]]	+	</StructureSection>
-	[[Category: Jedrzejczak, R]]	+	[[Category: Large Structures]]
-	[[Category: Wu, R]]	+	[[Category: Jedrzejczak R]]
		+	[[Category: Joachimiak A]]
		+	[[Category: Tan K]]
		+	[[Category: Wu R]]

OCA: Protected "7kb7" [edit=sysop:move=sysop]

2020-10-07T04:53:06Z

Protected "7kb7" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:53, 7 October 2020

OCA: New page: '''Unreleased structure''' The entry 7kb7 is ON HOLD Authors: Tan, K., Wu, R., Jedrzejczak, R., Joachimiak, A. Description: THE STRUCTURE OF A SENSOR DOMAIN OF A HISTIDINE KINASE (VxrA...

2020-10-07T04:53:05Z

New page: '''Unreleased structure''' The entry 7kb7 is ON HOLD Authors: Tan, K., Wu, R., Jedrzejczak, R., Joachimiak, A. Description: THE STRUCTURE OF A SENSOR DOMAIN OF A HISTIDINE KINASE (VxrA...

New page

'''Unreleased structure'''

The entry 7kb7 is ON HOLD

Authors: Tan, K., Wu, R., Jedrzejczak, R., Joachimiak, A.

Description: THE STRUCTURE OF A SENSOR DOMAIN OF A HISTIDINE KINASE (VxrA) FROM VIBRIO CHOLERAE O1 BIOVAR ELTOR STR. N16961, N239-T240 deletion mutant
[[Category: Unreleased Structures]]
[[Category: Joachimiak, A]]
[[Category: Tan, K]]
[[Category: Jedrzejczak, R]]
[[Category: Wu, R]]