7khm - Revision history

OCA at 15:25, 18 October 2023

2023-10-18T15:25:08Z

←Older revision		Revision as of 15:25, 18 October 2023
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	<StructureSection load='7khm' size='340' side='right'caption='[[7khm]], [[Resolution\|resolution]] 2.88Å' scene=''>		<StructureSection load='7khm' size='340' side='right'caption='[[7khm]], [[Resolution\|resolution]] 2.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7khm]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KHM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7khm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KHM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PKZ:PALMITOYL-COA'>PKZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.88Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PKZ:PALMITOYL-COA'>PKZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7khm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7khm OCA], [https://pdbe.org/7khm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7khm RCSB], [https://www.ebi.ac.uk/pdbsum/7khm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7khm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7khm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7khm OCA], [https://pdbe.org/7khm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7khm RCSB], [https://www.ebi.ac.uk/pdbsum/7khm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7khm ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ZDH20_HUMAN ZDH20_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Banerjee, A]]	+	[[Category: Banerjee A]]
-	[[Category: Lee, C J]]	+	[[Category: Lee C-J]]
-	~~[[Category: Acyl]]~~	+
-	~~[[Category: Dhhc]]~~	+
-	~~[[Category: Lipid]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Palmitoyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:39, 23 February 2022

2022-02-23T08:39:51Z

←Older revision		Revision as of 08:39, 23 February 2022
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	==Crystal structure of hDHHS20 bound to palmitoyl CoA==		==Crystal structure of hDHHS20 bound to palmitoyl CoA==
-	<StructureSection load='7khm' size='340' side='right'caption='[[7khm]]' scene=''>	+	<StructureSection load='7khm' size='340' side='right'caption='[[7khm]], [[Resolution\|resolution]] 2.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KHM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7khm]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KHM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7khm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7khm OCA], [https://pdbe.org/7khm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7khm RCSB], [https://www.ebi.ac.uk/pdbsum/7khm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7khm ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PKZ:PALMITOYL-COA'>PKZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7khm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7khm OCA], [https://pdbe.org/7khm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7khm RCSB], [https://www.ebi.ac.uk/pdbsum/7khm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7khm ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	S-acylation, also known as palmitoylation, is the most abundant form of protein lipidation in humans. This reversible posttranslational modification, which targets thousands of proteins, is catalyzed by 23 members of the DHHC family of integral membrane enzymes. DHHC enzymes use fatty acyl-CoA as the ubiquitous fatty acyl donor and become autoacylated at a catalytic cysteine; this intermediate subsequently transfers the fatty acyl group to a cysteine in the target protein. Protein S-acylation intersects with almost all areas of human physiology, and several DHHC enzymes are considered as possible therapeutic targets against diseases such as cancer. These efforts would greatly benefit from a detailed understanding of the molecular basis for this crucial enzymatic reaction. Here, we combine X-ray crystallography with all-atom molecular dynamics simulations to elucidate the structure of the precatalytic complex of human DHHC20 in complex with palmitoyl CoA. The resulting structure reveals that the fatty acyl chain inserts into a hydrophobic pocket within the transmembrane spanning region of the protein, whereas the CoA headgroup is recognized by the cytosolic domain through polar and ionic interactions. Biochemical experiments corroborate the predictions from our structural model. We show, using both computational and experimental analyses, that palmitoyl CoA acts as a bivalent ligand where the interaction of the DHHC enzyme with both the fatty acyl chain and the CoA headgroup is important for catalytic chemistry to proceed. This bivalency explains how, in the presence of high concentrations of free CoA under physiological conditions, DHHC enzymes can efficiently use palmitoyl CoA as a substrate for autoacylation.
		+
		+	Bivalent recognition of fatty acyl-CoA by a human integral membrane palmitoyltransferase.,Lee CJ, Stix R, Rana MS, Shikwana F, Murphy RE, Ghirlando R, Faraldo-Gomez JD, Banerjee A Proc Natl Acad Sci U S A. 2022 Feb 15;119(7). pii: 2022050119. doi:, 10.1073/pnas.2022050119. PMID:35140179<ref>PMID:35140179</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7khm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Banerjee A]]	+	[[Category: Banerjee, A]]
-	[[Category: Lee C-J]]	+	[[Category: Lee, C J]]
		+	[[Category: Acyl]]
		+	[[Category: Dhhc]]
		+	[[Category: Lipid]]
		+	[[Category: Membrane protein]]
		+	[[Category: Palmitoyltransferase]]
		+	[[Category: Transferase]]

OCA at 06:59, 2 February 2022

2022-02-02T06:59:10Z

←Older revision		Revision as of 06:59, 2 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7khm ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of hDHHS20 bound to palmitoyl CoA==
-		+	<StructureSection load='7khm' size='340' side='right'caption='[[7khm]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KHM FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7khm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7khm OCA], [https://pdbe.org/7khm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7khm RCSB], [https://www.ebi.ac.uk/pdbsum/7khm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7khm ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Banerjee A]]
		+	[[Category: Lee C-J]]

OCA at 04:56, 18 August 2021

2021-08-18T04:56:58Z

←Older revision		Revision as of 04:56, 18 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7khm is ON HOLD	+	The entry 7khm is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7khm" [edit=sysop:move=sysop]

2020-10-28T19:12:34Z

Protected "7khm" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:12, 28 October 2020

OCA: New page: '''Unreleased structure''' The entry 7khm is ON HOLD Authors: Description: Category: Unreleased Structures

2020-10-28T19:12:32Z

New page: '''Unreleased structure''' The entry 7khm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7khm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]