OCA at 07:07, 30 December 2020

2020-12-30T07:07:41Z

←Older revision		Revision as of 07:07, 30 December 2020
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	==Disulfide Stabilized Norovirus GI.1 VLP Shell Region==		==Disulfide Stabilized Norovirus GI.1 VLP Shell Region==
-	<StructureSection load='7kjp' size='340' side='right'caption='[[7kjp]]' scene=''>	+	<StructureSection load='7kjp' size='340' side='right'caption='[[7kjp]], [[Resolution\|resolution]] 3.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KJP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KJP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kjp]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hu/nv/nv/1968/us Hu/nv/nv/1968/us]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KJP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KJP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kjp OCA], [http://pdbe.org/7kjp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kjp RCSB], [http://www.ebi.ac.uk/pdbsum/7kjp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kjp ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=524364 Hu/NV/NV/1968/US])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kjp OCA], [http://pdbe.org/7kjp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kjp RCSB], [http://www.ebi.ac.uk/pdbsum/7kjp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kjp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAPSD_NVN68 CAPSD_NVN68]] Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells by binding histo-blood group antigens present on gastroduodenal epithelial cells.<ref>PMID:16840313</ref> Soluble capsid protein may play a role in viral immunoevasion.<ref>PMID:16840313</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human noroviruses are non-enveloped, single-strand RNA viruses that cause pandemic outbreaks of acute gastroenteritis. A bivalent vaccine containing GI.1 and GII.4 virus-like particles (VLPs) has been shown to be safe and highly immunogenic, but its efficacy and durability have been limited. Here, we show that norovirus GI.1 VLPs are unstable and contain a substantial fraction of dissociated VLP components. Broadly reactive, non-neutralizing antibodies isolated from vaccinated donors bound to the dissociated components, but not to the intact VLPs. Engineering of interprotomer disulfide bonds within the shell domain prevented disassembly of the VLPs, while preserving antibody accessibility to blockade epitopes. Without adjuvant, mice immunized with stabilized GI.1 VLPs developed faster blockade antibody titers compared to immunization with wild-type GI.1 VLPs. In addition, immunization with stabilized particles focused immune responses toward surface-exposed epitopes and away from occluded epitopes. Overall, disulfide-stabilized norovirus GI.1 VLPs elicited improved responses over the non-disulfide-stabilized version, suggesting their promise as candidate vaccines.
		+
		+	Disulfide stabilization of human norovirus GI.1 virus-like particles focuses immune response toward blockade epitopes.,Verardi R, Lindesmith LC, Tsybovsky Y, Gorman J, Chuang GY, Edwards CE, Brewer-Jensen PD, Mallory ML, Ou L, Schon A, Shi W, Tully ES, Georgiou G, Baric RS, Kwong PD NPJ Vaccines. 2020 Dec 14;5(1):110. doi: 10.1038/s41541-020-00260-w. PMID:33318483<ref>PMID:33318483</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kjp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hu/nv/nv/1968/us]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gorman J]]	+	[[Category: Gorman, J]]
-	[[Category: Kwong PD]]	+	[[Category: Kwong, P D]]
		+	[[Category: Norovirus]]
		+	[[Category: Shell]]
		+	[[Category: Stabilized]]
		+	[[Category: Virus like particle]]
		+	[[Category: Vlp]]

OCA at 07:57, 2 December 2020

2020-12-02T07:57:59Z

←Older revision		Revision as of 07:57, 2 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7kjp ~~is ON HOLD~~	+	==Disulfide Stabilized Norovirus GI.1 VLP Shell Region==
-		+	<StructureSection load='7kjp' size='340' side='right'caption='[[7kjp]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KJP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KJP FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kjp OCA], [http://pdbe.org/7kjp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kjp RCSB], [http://www.ebi.ac.uk/pdbsum/7kjp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kjp ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gorman J]]
		+	[[Category: Kwong PD]]

OCA: Protected "7kjp" [edit=sysop:move=sysop]

2020-11-04T05:39:24Z

Protected "7kjp" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 4 November 2020

OCA: New page: '''Unreleased structure''' The entry 7kjp is ON HOLD Authors: Description: Category: Unreleased Structures

2020-11-04T05:39:23Z

New page: '''Unreleased structure''' The entry 7kjp is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7kjp is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7kjp - Revision history

OCA at 07:07, 30 December 2020

OCA at 07:57, 2 December 2020

OCA: Protected "7kjp" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7kjp is ON HOLD Authors: Description: Category: Unreleased Structures