OCA at 15:26, 18 October 2023

2023-10-18T15:26:44Z

←Older revision		Revision as of 15:26, 18 October 2023
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	==Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor==		==Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor==
-	<StructureSection load='7kke' size='340' side='right'caption='[[7kke]]' scene=''>	+	<StructureSection load='7kke' size='340' side='right'caption='[[7kke]], [[Resolution\|resolution]] 2.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KKE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kke]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KKE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kke OCA], [https://pdbe.org/7kke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kke RCSB], [https://www.ebi.ac.uk/pdbsum/7kke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kke ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.81Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WJV:N-[2-(3,3-dimethylbutoxy)ethyl]-N-{4-methyl-5-[(pyridin-4-yl)ethynyl]-1,3-thiazol-2-yl}urea'>WJV</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kke OCA], [https://pdbe.org/7kke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kke RCSB], [https://www.ebi.ac.uk/pdbsum/7kke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kke ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PK3CG_HUMAN PK3CG_HUMAN] Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to ADRBK1 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contributes to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.<ref>PMID:7624799</ref> <ref>PMID:12163475</ref> <ref>PMID:15294162</ref> <ref>PMID:16094730</ref> <ref>PMID:21393242</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phosphoinositide 3-kinases (PI3Ks) are a family of enzymes that control a wide variety of cellular functions such as cell growth, proliferation, differentiation, motility, survival, and intracellular trafficking. PI3Kgamma plays a critical role in mediating leukocyte chemotaxis as well as mast cell degranulation, making it a potentially interesting target for autoimmune and inflammatory diseases. We previously disclosed a novel series of PI3Kgamma inhibitors derived from a benzothiazole core. The truncation of the benzothiazole core led to the discovery of a structurally diverse alkynyl thiazole series which displayed high PI3Kgamma potency and subtype selectivity. Further medicinal chemistry optimization of the alkynyl thiazole series led to identification of compounds such as 14 and 32, highly potent, subtype selective, and CNS penetrant PI3Kgamma inhibitors. Compound 14 showed robust inhibition of PI3Kgamma mediated neutrophil migration in vivo.
		+
		+	Discovery of a Novel Series of Potent and Selective Alkynylthiazole-Derived PI3Kgamma Inhibitors.,Bandarage UK, Aronov AM, Cao J, Come JH, Cottrell KM, Davies RJ, Giroux S, Jacobs M, Mahajan S, Messersmith D, Moody CS, Swett R, Xu J ACS Med Chem Lett. 2020 Dec 18;12(1):129-135. doi:, 10.1021/acsmedchemlett.0c00573. eCollection 2021 Jan 14. PMID:33488974<ref>PMID:33488974</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kke" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphoinositide 3-kinase 3D structures\|Phosphoinositide 3-kinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Griffith JP]]		[[Category: Griffith JP]]
	[[Category: Jacobs MD]]		[[Category: Jacobs MD]]

OCA at 06:41, 24 March 2021

2021-03-24T06:41:12Z

←Older revision		Revision as of 06:41, 24 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7kke ~~is ON HOLD until Paper Publication~~	+	==Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor==
-		+	<StructureSection load='7kke' size='340' side='right'caption='[[7kke]]' scene=''>
-	~~Authors~~: ~~Jacobs~~, M.D., ~~Griffith~~, J.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KKE FirstGlance]. <br>
-	~~Description~~: ~~Phosphoinositide 3~~-~~Kinase gamma bound to a thiazole inhibitor~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kke OCA], [https://pdbe.org/7kke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kke RCSB], [https://www.ebi.ac.uk/pdbsum/7kke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kke ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Jacobs, M.D~~]]	+	__TOC__
-	[[Category: ~~Griffith, J.P~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Griffith JP]]
		+	[[Category: Jacobs MD]]

OCA at 05:12, 9 December 2020

2020-12-09T05:12:29Z

←Older revision		Revision as of 05:12, 9 December 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7kke is ON HOLD	+	The entry 7kke is ON HOLD until Paper Publication

	Authors: Jacobs, M.D., Griffith, J.P.		Authors: Jacobs, M.D., Griffith, J.P.
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	Description: Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor		Description: Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Griffith, J.P]]
	[[Category: Jacobs, M.D]]		[[Category: Jacobs, M.D]]
		+	[[Category: Griffith, J.P]]

OCA: Protected "7kke" [edit=sysop:move=sysop]

2020-11-04T05:40:53Z

Protected "7kke" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:40, 4 November 2020

OCA: New page: '''Unreleased structure''' The entry 7kke is ON HOLD Authors: Jacobs, M.D., Griffith, J.P. Description: Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor [[Category: Unrele...

2020-11-04T05:40:52Z

New page: '''Unreleased structure''' The entry 7kke is ON HOLD Authors: Jacobs, M.D., Griffith, J.P. Description: Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 7kke is ON HOLD

Authors: Jacobs, M.D., Griffith, J.P.

Description: Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor
[[Category: Unreleased Structures]]
[[Category: Griffith, J.P]]
[[Category: Jacobs, M.D]]

7kke - Revision history

OCA at 15:26, 18 October 2023

OCA at 06:41, 24 March 2021

OCA at 05:12, 9 December 2020

OCA: Protected "7kke" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7kke is ON HOLD Authors: Jacobs, M.D., Griffith, J.P. Description: Phosphoinositide 3-Kinase gamma bound to a thiazole inhibitor [[Category: Unrele...