OCA at 15:30, 18 October 2023

2023-10-18T15:30:19Z

←Older revision		Revision as of 15:30, 18 October 2023
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-	====	+	==Ternary complex of TERT (telomerase reverse transcriptase) with RNA template, DNA primer, an incoming dGTP and a downstream hybrid duplex==
-	<StructureSection load='7kqn' size='340' side='right'caption='[[7kqn]]' scene=''>	+	<StructureSection load='7kqn' size='340' side='right'caption='[[7kqn]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kqn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Tribolium_castaneum Tribolium castaneum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KQN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kqn OCA], [https://pdbe.org/7kqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kqn RCSB], [https://www.ebi.ac.uk/pdbsum/7kqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kqn ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.02Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kqn OCA], [https://pdbe.org/7kqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kqn RCSB], [https://www.ebi.ac.uk/pdbsum/7kqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kqn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0QHL8_TRICA Q0QHL8_TRICA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Telomerase synthesizes telomeres at the ends of linear chromosomes by repeated reverse transcription from a short RNA template. Crystal structures of Tribolium castaneum telomerase reverse transcriptase (tcTERT) and cryoelectron microscopy (cryo-EM) structures of human and Tetrahymena telomerase have revealed conserved features in the reverse-transcriptase domain, including a cavity near the DNA 3' end and snug interactions with the RNA template. For the RNA template to translocate, it needs to be unpaired and separated from the DNA product. Here we investigate the potential of the structural cavity to accommodate a looped-out DNA bulge and enable the separation of the RNA/DNA hybrid. Using tcTERT as a model system, we show that a looped-out telomeric repeat in the DNA primer can be accommodated and extended by tcTERT but not by retroviral reverse transcriptase. Mutations that reduce the cavity size reduce the ability of tcTERT to extend the looped-out DNA substrate. In agreement with cryo-EM structures of telomerases, we find that tcTERT requires a minimum of 4 bp between the RNA template and DNA primer for efficient DNA synthesis. We also have determined the ternary-complex structure of tcTERT including a downstream RNA/DNA hybrid at 2.0-A resolution and shown that a downstream RNA duplex, equivalent to the 5' template-boundary element in telomerase RNA, enhances the efficiency of telomere synthesis by tcTERT. Although TERT has a preformed active site without the open-and-closed conformational changes, it contains cavities to accommodate looped-out RNA and DNA. The flexible RNA-DNA binding likely underlies the processivity of telomeric repeat addition.
		+
		+	Flexibility of telomerase in binding the RNA template and DNA telomeric repeat.,Choi WS, Weng PJ, Yang W Proc Natl Acad Sci U S A. 2022 Jan 4;119(1):e2116159118. doi: , 10.1073/pnas.2116159118. PMID:34969861<ref>PMID:34969861</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kqn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Telomerase 3D structures\|Telomerase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Tribolium castaneum]]
		+	[[Category: Choi WS]]
		+	[[Category: Weng PJ]]
		+	[[Category: Yang W]]

OCA at 11:16, 23 March 2022

2022-03-23T11:16:56Z

←Older revision		Revision as of 11:16, 23 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7kqn ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7kqn' size='340' side='right'caption='[[7kqn]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kqn OCA], [https://pdbe.org/7kqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kqn RCSB], [https://www.ebi.ac.uk/pdbsum/7kqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kqn ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:12, 10 February 2021

2021-02-10T09:12:37Z

←Older revision		Revision as of 09:12, 10 February 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7kqn is ON HOLD	+	The entry 7kqn is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7kqn" [edit=sysop:move=sysop]

2020-11-25T05:33:28Z

Protected "7kqn" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:33, 25 November 2020

OCA: New page: '''Unreleased structure''' The entry 7kqn is ON HOLD Authors: Description: Category: Unreleased Structures

2020-11-25T05:33:27Z

New page: '''Unreleased structure''' The entry 7kqn is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7kqn is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7kqn - Revision history

OCA at 15:30, 18 October 2023

OCA at 11:16, 23 March 2022

OCA at 09:12, 10 February 2021

OCA: Protected "7kqn" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7kqn is ON HOLD Authors: Description: Category: Unreleased Structures