7l6g - Revision history

OCA at 10:10, 14 July 2021

2021-07-14T10:10:11Z

←Older revision		Revision as of 10:10, 14 July 2021
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l6g OCA], [https://pdbe.org/7l6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l6g RCSB], [https://www.ebi.ac.uk/pdbsum/7l6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l6g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l6g OCA], [https://pdbe.org/7l6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l6g RCSB], [https://www.ebi.ac.uk/pdbsum/7l6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l6g ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Some methane-oxidizing bacteria use the ribosomally synthesized, posttranslationally modified natural product methanobactin (Mbn) to acquire copper for their primary metabolic enzyme, particulate methane monooxygenase. The operons encoding the machinery to biosynthesize and transport Mbns typically include genes for two proteins, MbnH and MbnP, which are also found as a pair in other genomic contexts related to copper homeostasis. While the MbnH protein, a member of the bacterial diheme cytochrome c peroxidase (bCcP)/MauG superfamily, has been characterized, the structure and function of MbnP, the relationship between the two proteins, and their role in copper homeostasis remain unclear. Biochemical characterization of MbnP from the methanotroph Methylosinus trichosporium OB3b now reveals that MbnP binds a single copper ion, present in the +1 oxidation state, with high affinity. Copper binding to MbnP in vivo is dependent on oxidation of the first tryptophan in a conserved WxW motif to a kynurenine, a transformation that occurs through an interaction of MbnH with MbnP. The 2.04-A-resolution crystal structure of MbnP reveals a unique fold and an unusual copper-binding site involving a histidine, a methionine, a solvent ligand, and the kynurenine. Although the kynurenine residue may not serve as a Cu(I) primary-sphere ligand, being positioned approximately 2.9 A away from the Cu(I) ion, its presence is required for copper binding. Genomic neighborhood analysis indicates that MbnP proteins, and by extension kynurenine-containing copper sites, are widespread and may play diverse roles in microbial copper homeostasis.
		+
		+	Copper binding by a unique family of metalloproteins is dependent on kynurenine formation.,Manesis AC, Jodts RJ, Hoffman BM, Rosenzweig AC Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2100680118. doi:, 10.1073/pnas.2100680118. PMID:34074779<ref>PMID:34074779</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7l6g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:34, 9 June 2021

2021-06-09T12:34:14Z

←Older revision		Revision as of 12:34, 9 June 2021
Line 1:		Line 1:

	==MbnP from Methylosinus trichosporium==		==MbnP from Methylosinus trichosporium==
-	<StructureSection load='7l6g' size='340' side='right'caption='[[7l6g]]' scene=''>	+	<StructureSection load='7l6g' size='340' side='right'caption='[[7l6g]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7l6g]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylosinus_trichosporium_ob3b Methylosinus trichosporium ob3b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L6G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l6g OCA], [https://pdbe.org/7l6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l6g RCSB], [https://www.ebi.ac.uk/pdbsum/7l6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l6g ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CQW49_07140 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=595536 Methylosinus trichosporium OB3b])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l6g OCA], [https://pdbe.org/7l6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l6g RCSB], [https://www.ebi.ac.uk/pdbsum/7l6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l6g ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Manesis AC]]	+	[[Category: Methylosinus trichosporium ob3b]]
-	[[Category: Rosenzweig AC]]	+	[[Category: Manesis, A C]]
		+	[[Category: Rosenzweig, A C]]
		+	[[Category: Copper]]
		+	[[Category: Kynurenine]]
		+	[[Category: Mbnp]]
		+	[[Category: Metal binding protein]]

OCA at 09:20, 26 May 2021

2021-05-26T09:20:26Z

←Older revision		Revision as of 09:20, 26 May 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7l6g ~~is ON HOLD until Paper Publication~~	+	==MbnP from Methylosinus trichosporium==
-		+	<StructureSection load='7l6g' size='340' side='right'caption='[[7l6g]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L6G FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l6g OCA], [https://pdbe.org/7l6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l6g RCSB], [https://www.ebi.ac.uk/pdbsum/7l6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l6g ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Manesis AC]]
		+	[[Category: Rosenzweig AC]]

OCA at 09:13, 10 February 2021

2021-02-10T09:13:23Z

←Older revision		Revision as of 09:13, 10 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7l6g is ON HOLD	+	The entry 7l6g is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7l6g" [edit=sysop:move=sysop]

2020-12-30T05:33:25Z

Protected "7l6g" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:33, 30 December 2020

OCA: New page: '''Unreleased structure''' The entry 7l6g is ON HOLD Authors: Description: Category: Unreleased Structures

2020-12-30T05:33:24Z

New page: '''Unreleased structure''' The entry 7l6g is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7l6g is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]