7low - Revision history

OCA at 15:55, 18 October 2023

2023-10-18T15:55:03Z

←Older revision		Revision as of 15:55, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7low]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_908573 Escherichia coli 908573]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOW FirstGlance]. <br>		<table><tr><td colspan='2'>[[7low]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_908573 Escherichia coli 908573]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.39Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metK, HMPREF1611_00479 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1268998 Escherichia coli 908573])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7low FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7low OCA], [https://pdbe.org/7low PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7low RCSB], [https://www.ebi.ac.uk/pdbsum/7low PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7low ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7low FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7low OCA], [https://pdbe.org/7low PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7low RCSB], [https://www.ebi.ac.uk/pdbsum/7low PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7low ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX]] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]	+	[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7low" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7low" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[S-adenosylmethionine synthetase 3D structures\|S-adenosylmethionine synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Escherichia coli 908573]]		[[Category: Escherichia coli 908573]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Methionine adenosyltransferase]]~~	+	[[Category: Jackson CJ]]
-	[[Category: Jackson~~, C J~~]]	+	[[Category: Tan LL]]
-	[[Category: Tan~~, L L]]~~	+
-	~~[[Category: S-adenosylmethionine synthetase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:28, 2 March 2022

2022-03-02T07:28:16Z

←Older revision		Revision as of 07:28, 2 March 2022
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	==S-adenosylmethionine synthetase==		==S-adenosylmethionine synthetase==
-	<StructureSection load='7low' size='340' side='right'caption='[[7low]]' scene=''>	+	<StructureSection load='7low' size='340' side='right'caption='[[7low]], [[Resolution\|resolution]] 2.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7low]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_908573 Escherichia coli 908573]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7low FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7low OCA], [https://pdbe.org/7low PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7low RCSB], [https://www.ebi.ac.uk/pdbsum/7low PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7low ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metK, HMPREF1611_00479 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1268998 Escherichia coli 908573])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7low FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7low OCA], [https://pdbe.org/7low PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7low RCSB], [https://www.ebi.ac.uk/pdbsum/7low PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7low ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX]] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein conformational changes can facilitate the binding of noncognate substrates and underlying promiscuous activities. However, the contribution of substrate conformational dynamics to this process is comparatively poorly understood. Here, we analyze human (hMAT2A) and Escherichia coli (eMAT) methionine adenosyltransferases that have identical active sites but different substrate specificity. In the promiscuous hMAT2A, noncognate substrates bind in a stable conformation to allow catalysis. In contrast, noncognate substrates sample stable productive binding modes less frequently in eMAT owing to altered mobility in the enzyme active site. Different cellular concentrations of substrates likely drove the evolutionary divergence of substrate specificity in these orthologues. The observation of catalytic promiscuity in hMAT2A led to the detection of a new human metabolite, methyl thioguanosine, that is produced at elevated levels in a cancer cell line. This work establishes that identical active sites can result in different substrate specificity owing to the effects of substrate and enzyme dynamics.
		+
		+	Substrate Dynamics Contribute to Enzymatic Specificity in Human and Bacterial Methionine Adenosyltransferases.,Gade M, Tan LL, Damry AM, Sandhu M, Brock JS, Delaney A, Villar-Briones A, Jackson CJ, Laurino P JACS Au. 2021 Nov 19;1(12):2349-2360. doi: 10.1021/jacsau.1c00464. eCollection, 2021 Dec 27. PMID:34977903<ref>PMID:34977903</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7low" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli 908573]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jackson CJ]]	+	[[Category: Methionine adenosyltransferase]]
-	[[Category: Tan LL]]	+	[[Category: Jackson, C J]]
		+	[[Category: Tan, L L]]
		+	[[Category: S-adenosylmethionine synthetase]]
		+	[[Category: Transferase]]

OCA at 10:34, 31 March 2021

2021-03-31T10:34:05Z

←Older revision		Revision as of 10:34, 31 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7low ~~is ON HOLD until Feb 10 2023~~	+	==S-adenosylmethionine synthetase==
-		+	<StructureSection load='7low' size='340' side='right'caption='[[7low]]' scene=''>
-	~~Authors~~: ~~Tan~~, L.L., ~~Jackson~~, C.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOW FirstGlance]. <br>
-	~~Description~~: S-~~adenosylmethionine synthetase~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7low FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7low OCA], [https://pdbe.org/7low PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7low RCSB], [https://www.ebi.ac.uk/pdbsum/7low PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7low ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Jackson~~, C.J~~]]	+	__TOC__
-	[[Category: Tan~~, L.L~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Jackson CJ]]
		+	[[Category: Tan LL]]

OCA at 05:22, 17 March 2021

2021-03-17T05:22:40Z

←Older revision		Revision as of 05:22, 17 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7low is ON HOLD	+	The entry 7low is ON HOLD until Feb 10 2023

	Authors: Tan, L.L., Jackson, C.J.		Authors: Tan, L.L., Jackson, C.J.

OCA: Protected "7low" [edit=sysop:move=sysop]

2021-02-17T08:49:46Z

Protected "7low" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:49, 17 February 2021

OCA: New page: '''Unreleased structure''' The entry 7low is ON HOLD Authors: Tan, L.L., Jackson, C.J. Description: S-adenosylmethionine synthetase Category: Unreleased Structures [[Category: Jack...

2021-02-17T08:49:45Z

New page: '''Unreleased structure''' The entry 7low is ON HOLD Authors: Tan, L.L., Jackson, C.J. Description: S-adenosylmethionine synthetase Category: Unreleased Structures [[Category: Jack...

New page

'''Unreleased structure'''

The entry 7low is ON HOLD

Authors: Tan, L.L., Jackson, C.J.

Description: S-adenosylmethionine synthetase
[[Category: Unreleased Structures]]
[[Category: Jackson, C.J]]
[[Category: Tan, L.L]]