OCA at 06:34, 25 August 2021

2021-08-25T06:34:14Z

←Older revision		Revision as of 06:34, 25 August 2021
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	==Apo Rr RsiG- crystal form 1==		==Apo Rr RsiG- crystal form 1==
-	<StructureSection load='7lq2' size='340' side='right'caption='[[7lq2]]' scene=''>	+	<StructureSection load='7lq2' size='340' side='right'caption='[[7lq2]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LQ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LQ2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lq2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_51242 Atcc 51242]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LQ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LQ2 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lq2 OCA], [https://pdbe.org/7lq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lq2 RCSB], [https://www.ebi.ac.uk/pdbsum/7lq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lq2 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RradSPS_1442 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42256 ATCC 51242])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lq2 OCA], [https://pdbe.org/7lq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lq2 RCSB], [https://www.ebi.ac.uk/pdbsum/7lq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lq2 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Filamentous actinobacteria of the genus Streptomyces have a complex lifecycle involving the differentiation of reproductive aerial hyphae into spores. We recently showed c-di-GMP controls this transition by arming a unique anti-sigma, RsiG, to bind the sporulation-specific sigma, WhiG. The Streptomyces venezuelae RsiG-(c-di-GMP)2-WhiG structure revealed that a monomeric RsiG binds c-di-GMP via two E(X)3S(X)2R(X)3Q(X)3D repeat motifs, one on each helix of an antiparallel coiled-coil. Here we show that RsiG homologs are found scattered throughout the Actinobacteria. Strikingly, RsiGs from unicellular bacteria descending from the most basal branch of the Actinobacteria are small proteins containing only one c-di-GMP binding motif, yet still bind their WhiG partners. Our structure of a Rubrobacter radiotolerans (RsiG)2-(c-di-GMP)2-WhiG complex revealed that these single-motif RsiGs are able to form an antiparallel coiled-coil through homodimerization, thereby allowing them to bind c-di-GMP similar to the monomeric twin-motif RsiGs. Further data show that in the unicellular actinobacterium R. radiotolerans, the (RsiG)2-(c-di-GMP)2-WhiG regulatory switch controls type IV pilus expression. Phylogenetic analysis indicates the single-motif RsiGs likely represent the ancestral state and an internal gene-duplication event gave rise to the twin-motif RsiGs inherited elsewhere in the Actinobacteria. Thus, these studies show how the anti-sigma RsiG has evolved through an intragenic duplication event from a small protein carrying a single c-di-GMP binding motif, which functions as a homodimer, to a larger protein carrying two c-di-GMP binding motifs, which functions as a monomer. Consistent with this, our structures reveal potential selective advantages of the monomeric twin-motif anti-sigma factors.
		+
		+	Evolution of a sigma-(c-di-GMP)-anti-sigma switch.,Schumacher MA, Gallagher KA, Holmes NA, Chandra G, Henderson M, Kysela DT, Brennan RG, Buttner MJ Proc Natl Acad Sci U S A. 2021 Jul 27;118(30). pii: 2105447118. doi:, 10.1073/pnas.2105447118. PMID:34290147<ref>PMID:34290147</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lq2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 51242]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brennan RG]]	+	[[Category: Brennan, R G]]
-	[[Category: Schumacher MA]]	+	[[Category: Schumacher, M A]]
		+	[[Category: Evolution]]
		+	[[Category: Homodimer]]
		+	[[Category: Rsig]]
		+	[[Category: Rubrobacter radiotoleran]]
		+	[[Category: Single-motif]]
		+	[[Category: Transcription]]
		+	[[Category: Whig]]

OCA at 09:54, 14 July 2021

2021-07-14T09:54:41Z

←Older revision		Revision as of 09:54, 14 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7lq2 ~~is ON HOLD until Paper Publication~~	+	==Apo Rr RsiG- crystal form 1==
-		+	<StructureSection load='7lq2' size='340' side='right'caption='[[7lq2]]' scene=''>
-	~~Authors~~: ~~Schumacher~~, M.A., ~~Brennan~~, R.G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LQ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LQ2 FirstGlance]. <br>
-	~~Description~~: ~~Apo Rr RsiG~~-~~crystal form 1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lq2 OCA], [https://pdbe.org/7lq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lq2 RCSB], [https://www.ebi.ac.uk/pdbsum/7lq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lq2 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Brennan~~, R.G~~]]	+	__TOC__
-	[[Category: Schumacher~~, M.A~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Brennan RG]]
		+	[[Category: Schumacher MA]]

OCA: Protected "7lq2" [edit=sysop:move=sysop]

2021-02-24T05:39:56Z

Protected "7lq2" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 24 February 2021

OCA: New page: '''Unreleased structure''' The entry 7lq2 is ON HOLD until Paper Publication Authors: Schumacher, M.A., Brennan, R.G. Description: Apo Rr RsiG-crystal form 1 [[Category: Unreleased Str...

2021-02-24T05:39:55Z

New page: '''Unreleased structure''' The entry 7lq2 is ON HOLD until Paper Publication Authors: Schumacher, M.A., Brennan, R.G. Description: Apo Rr RsiG-crystal form 1 [[Category: Unreleased Str...

New page

'''Unreleased structure'''

The entry 7lq2 is ON HOLD until Paper Publication

Authors: Schumacher, M.A., Brennan, R.G.

Description: Apo Rr RsiG-crystal form 1
[[Category: Unreleased Structures]]
[[Category: Brennan, R.G]]
[[Category: Schumacher, M.A]]

7lq2 - Revision history

OCA at 06:34, 25 August 2021

OCA at 09:54, 14 July 2021

OCA: Protected "7lq2" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7lq2 is ON HOLD until Paper Publication Authors: Schumacher, M.A., Brennan, R.G. Description: Apo Rr RsiG-crystal form 1 [[Category: Unreleased Str...