7lrn - Revision history

OCA at 15:57, 18 October 2023

2023-10-18T15:57:37Z

←Older revision		Revision as of 15:57, 18 October 2023
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	<StructureSection load='7lrn' size='340' side='right'caption='[[7lrn]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='7lrn' size='340' side='right'caption='[[7lrn]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7lrn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aciba Aciba~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LRN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lrn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LRN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.85Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~A7M79_07860, BGC29_02895 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 ACIBA])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lrn OCA], [https://pdbe.org/7lrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lrn RCSB], [https://www.ebi.ac.uk/pdbsum/7lrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lrn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lrn OCA], [https://pdbe.org/7lrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lrn RCSB], [https://www.ebi.ac.uk/pdbsum/7lrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lrn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1E3M8P2_ACIBA A0A1E3M8P2_ACIBA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aciba~~]]	+	[[Category: Acinetobacter baumannii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Korasick~~, D A~~]]	+	[[Category: Korasick DA]]
-	[[Category: Tanner~~, J J]]~~	+	[[Category: Tanner JJ]]
-	~~[[Category: Flavin binding]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Siderophore-interacting protein~~]]	+

OCA at 06:34, 25 August 2021

2021-08-25T06:34:28Z

←Older revision		Revision as of 06:34, 25 August 2021
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	==Structure of the Siderophore Interacting Protein from Acinetbacter baumannii==		==Structure of the Siderophore Interacting Protein from Acinetbacter baumannii==
-	<StructureSection load='7lrn' size='340' side='right'caption='[[7lrn]]' scene=''>	+	<StructureSection load='7lrn' size='340' side='right'caption='[[7lrn]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LRN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lrn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aciba Aciba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LRN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lrn OCA], [https://pdbe.org/7lrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lrn RCSB], [https://www.ebi.ac.uk/pdbsum/7lrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lrn ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A7M79_07860, BGC29_02895 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 ACIBA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lrn OCA], [https://pdbe.org/7lrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lrn RCSB], [https://www.ebi.ac.uk/pdbsum/7lrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lrn ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acinetobacter baumannii is an opportunistic pathogen with a high mortality rate due to multi-drug-resistant strains. The synthesis and uptake of the iron-chelating siderophores acinetobactin (Acb) and preacinetobactin (pre-Acb) have been shown to be essential for virulence. Here, we report the kinetic and structural characterization of BauF, a flavin-dependent siderophore-interacting protein (SIP) required for the reduction of Fe(III) bound to Acb/pre-Acb and release of Fe(II). Stopped-flow spectrophotometric studies of the reductive half-reaction show that BauF forms a stable neutral flavin semiquinone intermediate. Reduction with NAD(P)H is very slow (k obs, 0.001 s(-1)) and commensurate with the rate of reduction by photobleaching, suggesting that NAD(P)H are not the physiological partners of BauF. The reduced BauF was oxidized by Acb-Fe (k obs, 0.02 s(-1)) and oxazole pre-Acb-Fe (ox-pre-Acb-Fe) (k obs, 0.08 s(-1)), a rigid analogue of pre-Acb, at a rate 3-11 times faster than that with molecular oxygen alone. The structure of FAD-bound BauF was solved at 2.85 A and was found to share a similarity to Shewanella SIPs. The biochemical and structural data presented here validate the role of BauF in A. baumannii iron assimilation and provide information important for drug design.
		+
		+	Structural and Biochemical Characterization of the Flavin-Dependent Siderophore-Interacting Protein from Acinetobacter baumannii.,Valentino H, Korasick DA, Bohac TJ, Shapiro JA, Wencewicz TA, Tanner JJ, Sobrado P ACS Omega. 2021 Jul 6;6(28):18537-18547. doi: 10.1021/acsomega.1c03047., eCollection 2021 Jul 20. PMID:34308084<ref>PMID:34308084</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lrn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aciba]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Korasick DA]]	+	[[Category: Korasick, D A]]
-	[[Category: Tanner JJ]]	+	[[Category: Tanner, J J]]
		+	[[Category: Flavin binding]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Siderophore-interacting protein]]

OCA at 08:58, 21 July 2021

2021-07-21T08:58:23Z

←Older revision		Revision as of 08:58, 21 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7lrn ~~is ON HOLD until Paper Publication~~	+	==Structure of the Siderophore Interacting Protein from Acinetbacter baumannii==
-		+	<StructureSection load='7lrn' size='340' side='right'caption='[[7lrn]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LRN FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lrn OCA], [https://pdbe.org/7lrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lrn RCSB], [https://www.ebi.ac.uk/pdbsum/7lrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lrn ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Korasick DA]]
		+	[[Category: Tanner JJ]]

OCA: Protected "7lrn" [edit=sysop:move=sysop]

2021-02-24T05:42:26Z

Protected "7lrn" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:42, 24 February 2021

OCA: New page: '''Unreleased structure''' The entry 7lrn is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2021-02-24T05:42:25Z

New page: '''Unreleased structure''' The entry 7lrn is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7lrn is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]