7lux - Revision history

OCA at 15:00, 6 March 2024

2024-03-06T15:00:42Z

←Older revision		Revision as of 15:00, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='7lux' size='340' side='right'caption='[[7lux]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='7lux' size='340' side='right'caption='[[7lux]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7lux]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lux]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.303Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[7ltu\|7ltu]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lux OCA], [https://pdbe.org/7lux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lux RCSB], [https://www.ebi.ac.uk/pdbsum/7lux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lux ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lux OCA], [https://pdbe.org/7lux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lux RCSB], [https://www.ebi.ac.uk/pdbsum/7lux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lux ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2]] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.	+	[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The SARS-CoV-2 Nucleoprotein (NCAP) functions in RNA packaging during viral replication and assembly. Computational analysis of its amino acid sequence reveals a central low-complexity domain (LCD) having sequence features akin to LCDs in other proteins known to function in liquid-liquid phase separation. Here we show that in the presence of viral RNA, NCAP, and also its LCD segment alone, form amyloid-like fibrils when undergoing liquid-liquid phase separation. Within the LCD we identified three 6-residue segments that drive amyloid fibril formation. We determined atomic structures for fibrils formed by each of the three identified segments. These structures informed our design of peptide inhibitors of NCAP fibril formation and liquid-liquid phase separation, suggesting a therapeutic route for Covid-19. One Sentence Summary: Atomic structures of amyloid-driving peptide segments from SARS-CoV-2 Nucleoprotein inform the development of Covid-19 therapeutics.	+
-		+
-	Inhibition of amyloid formation of the Nucleoprotein of SARS-CoV-2.,Tayeb-Fligelman E, Cheng X, Tai C, Bowler JT, Griner S, Sawaya MR, Seidler PM, Jiang YX, Lu J, Rosenberg GM, Salwinski L, Abskharon R, Zee CT, Hou K, Li Y, Boyer DR, Murray KA, Falcon G, Anderson DH, Cascio D, Saelices L, Damoiseaux R, Guo F, Eisenberg DS bioRxiv. 2021 Mar 5. doi: 10.1101/2021.03.05.434000. PMID:33688654<ref>PMID:33688654</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 7lux" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eisenberg~~, D S~~]]	+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
-	[[Category: Lu, J]]	+	[[Category: Eisenberg DS]]
-	[[Category: Rodriguez~~, J A~~]]	+	[[Category: Lu J]]
-	[[Category: Sawaya~~, M R~~]]	+	[[Category: Rodriguez JA]]
-	[[Category: Zee, C T]]	+	[[Category: Sawaya MR]]
-	~~[[Category: Amyloid fibril]]~~	+	[[Category: Zee C-T]]
-	~~[[Category: Protein fibril~~]]	+

OCA at 06:55, 24 March 2021

2021-03-24T06:55:03Z

←Older revision		Revision as of 06:55, 24 March 2021
Line 1:		Line 1:

	==AALALL segment from the Nucleoprotein of SARS-CoV-2, residues 217-222, crystal form 2==		==AALALL segment from the Nucleoprotein of SARS-CoV-2, residues 217-222, crystal form 2==
-	<StructureSection load='7lux' size='340' side='right'caption='[[7lux]]' scene=''>	+	<StructureSection load='7lux' size='340' side='right'caption='[[7lux]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lux]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lux OCA], [https://pdbe.org/7lux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lux RCSB], [https://www.ebi.ac.uk/pdbsum/7lux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lux ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7ltu\|7ltu]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lux OCA], [https://pdbe.org/7lux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lux RCSB], [https://www.ebi.ac.uk/pdbsum/7lux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lux ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2]] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The SARS-CoV-2 Nucleoprotein (NCAP) functions in RNA packaging during viral replication and assembly. Computational analysis of its amino acid sequence reveals a central low-complexity domain (LCD) having sequence features akin to LCDs in other proteins known to function in liquid-liquid phase separation. Here we show that in the presence of viral RNA, NCAP, and also its LCD segment alone, form amyloid-like fibrils when undergoing liquid-liquid phase separation. Within the LCD we identified three 6-residue segments that drive amyloid fibril formation. We determined atomic structures for fibrils formed by each of the three identified segments. These structures informed our design of peptide inhibitors of NCAP fibril formation and liquid-liquid phase separation, suggesting a therapeutic route for Covid-19. One Sentence Summary: Atomic structures of amyloid-driving peptide segments from SARS-CoV-2 Nucleoprotein inform the development of Covid-19 therapeutics.
		+
		+	Inhibition of amyloid formation of the Nucleoprotein of SARS-CoV-2.,Tayeb-Fligelman E, Cheng X, Tai C, Bowler JT, Griner S, Sawaya MR, Seidler PM, Jiang YX, Lu J, Rosenberg GM, Salwinski L, Abskharon R, Zee CT, Hou K, Li Y, Boyer DR, Murray KA, Falcon G, Anderson DH, Cascio D, Saelices L, Damoiseaux R, Guo F, Eisenberg DS bioRxiv. 2021 Mar 5. doi: 10.1101/2021.03.05.434000. PMID:33688654<ref>PMID:33688654</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lux" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eisenberg DS]]	+	[[Category: Eisenberg, D S]]
-	[[Category: Lu J]]	+	[[Category: Lu, J]]
-	[[Category: Rodriguez JA]]	+	[[Category: Rodriguez, J A]]
-	[[Category: Sawaya MR]]	+	[[Category: Sawaya, M R]]
-	[[Category: Zee C-T]]	+	[[Category: Zee, C T]]
		+	[[Category: Amyloid fibril]]
		+	[[Category: Protein fibril]]

OCA at 06:49, 17 March 2021

2021-03-17T06:49:15Z

←Older revision		Revision as of 06:49, 17 March 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7lux is ON HOLD until Paper Publication~~	+	==AALALL segment from the Nucleoprotein of SARS-CoV-2, residues 217-222, crystal form 2==
-		+	<StructureSection load='7lux' size='340' side='right'caption='[[7lux]]' scene=''>
-	~~Authors: Lu, J., Zee, C.-T., Sawaya, M.R., Rodriguez, J.A., Eisenberg, D.S.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUX FirstGlance]. <br>
-	~~Description:~~ AALALL segment from the Nucleoprotein of SARS-CoV-2, residues 217-222, crystal form 2	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lux OCA], [https://pdbe.org/7lux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lux RCSB], [https://www.ebi.ac.uk/pdbsum/7lux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lux ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Sawaya~~, M.R]]	+	__TOC__
-	[[~~Category~~: ~~Zee~~, C.-T]]	+	</StructureSection>
-	[[Category: Eisenberg~~, D.S~~]]	+	[[Category: Large Structures]]
-	[[Category: Lu, J]]	+	[[Category: Eisenberg DS]]
-	[[Category: Rodriguez~~, J.A~~]]	+	[[Category: Lu J]]
		+	[[Category: Rodriguez JA]]
		+	[[Category: Sawaya MR]]
		+	[[Category: Zee C-T]]

OCA: Protected "7lux" [edit=sysop:move=sysop]

2021-03-03T13:28:04Z

Protected "7lux" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:28, 3 March 2021

OCA: New page: '''Unreleased structure''' The entry 7lux is ON HOLD until Paper Publication Authors: Lu, J., Zee, C.-T., Sawaya, M.R., Rodriguez, J.A., Eisenberg, D.S. Description: AALALL segment fro...

2021-03-03T13:28:03Z

New page: '''Unreleased structure''' The entry 7lux is ON HOLD until Paper Publication Authors: Lu, J., Zee, C.-T., Sawaya, M.R., Rodriguez, J.A., Eisenberg, D.S. Description: AALALL segment fro...

New page

'''Unreleased structure'''

The entry 7lux is ON HOLD until Paper Publication

Authors: Lu, J., Zee, C.-T., Sawaya, M.R., Rodriguez, J.A., Eisenberg, D.S.

Description: AALALL segment from the Nucleoprotein of SARS-CoV-2, residues 217-222, crystal form 2
[[Category: Unreleased Structures]]
[[Category: Sawaya, M.R]]
[[Category: Zee, C.-T]]
[[Category: Eisenberg, D.S]]
[[Category: Lu, J]]
[[Category: Rodriguez, J.A]]