7m1l - Revision history

OCA at 16:03, 18 October 2023

2023-10-18T16:03:44Z

←Older revision		Revision as of 16:03, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='7m1l' size='340' side='right'caption='[[7m1l]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='7m1l' size='340' side='right'caption='[[7m1l]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7m1l]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M1L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7m1l]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M1L FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>clpP2, clpP, PAMH19_1747 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~287~~ "~~Bacillus aeruginosus~~" ~~(Schroeter 1872) Trevisan 1885])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Endopeptidase_Clp Endopeptidase Clp], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.92 3.4.21.92]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m1l OCA], [https://pdbe.org/7m1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m1l RCSB], [https://www.ebi.ac.uk/pdbsum/7m1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m1l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m1l OCA], [https://pdbe.org/7m1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m1l RCSB], [https://www.ebi.ac.uk/pdbsum/7m1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m1l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~A0A0A8RGC1_PSEAI A0A0A8RGC1_PSEAI]~~] Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.[HAMAP-Rule:MF_00444]	+	[https://www.uniprot.org/uniprot/CLPP2_PSEAE CLPP2_PSEAE] Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.[HAMAP-Rule:MF_00444]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	==See Also==		==See Also==
	*[[Clp protease 3D structures\|Clp protease 3D structures]]		*[[Clp protease 3D structures\|Clp protease 3D structures]]
		+	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Endopeptidase Clp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Baker, T A~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Grant, R A~~]]	+	[[Category: Baker TA]]
-	[[Category: ~~Hall, B M~~]]	+	[[Category: Grant RA]]
-	[[Category: ~~Sauer, R T~~]]	+	[[Category: Hall BM]]
-	[[Category: ~~Hydrolase]]~~	+	[[Category: Sauer RT]]
-	~~[[Category: Petidase~~]]	+

OCA at 03:51, 2 July 2021

2021-07-02T03:51:32Z

←Older revision		Revision as of 03:51, 2 July 2021
Line 1:		Line 1:

	==Crystal structure of Pseudomonas aeruginosa ClpP2==		==Crystal structure of Pseudomonas aeruginosa ClpP2==
-	<StructureSection load='7m1l' size='340' side='right'caption='[[7m1l]]' scene=''>	+	<StructureSection load='7m1l' size='340' side='right'caption='[[7m1l]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M1L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7m1l]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M1L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m1l OCA], [https://pdbe.org/7m1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m1l RCSB], [https://www.ebi.ac.uk/pdbsum/7m1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m1l ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clpP2, clpP, PAMH19_1747 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Endopeptidase_Clp Endopeptidase Clp], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.92 3.4.21.92] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m1l OCA], [https://pdbe.org/7m1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m1l RCSB], [https://www.ebi.ac.uk/pdbsum/7m1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m1l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A0A8RGC1_PSEAI A0A0A8RGC1_PSEAI]] Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.[HAMAP-Rule:MF_00444]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Caseinolytic proteases (Clp) are central to bacterial proteolysis and control cellular physiology and stress responses. They are composed of a double-ring compartmentalized peptidase (ClpP) and a AAA+ unfoldase (ClpX or ClpA/ClpC). Unlike many bacteria, the opportunistic pathogen Pseudomonas aeruginosa contains two ClpP homologs: ClpP1 and ClpP2. The specific functions of these homologs, however, are largely elusive. Here, we report that the active form of PaClpP2 is a part of a heteromeric PaClpP17 P27 tetradecamer that is required for proper biofilm development. PaClpP114 and PaClpP17 P27 complexes exhibit distinct peptide cleavage specificities and interact differentially with P. aeruginosa ClpX and ClpA. Crystal structures reveal that PaClpP2 has non-canonical features in its N- and C-terminal regions that explain its poor interaction with unfoldases. However, experiments in vivo indicate that the PaClpP2 peptidase active site uniquely contributes to biofilm development. These data strongly suggest that the specificity of different classes of ClpP peptidase subunits contributes to the biological outcome of proteolysis. This specialized role of PaClpP2 highlights it as an attractive target for developing antimicrobial agents that interfere specifically with late-stage P. aeruginosa development.
		+
		+	ClpP1P2 peptidase activity promotes biofilm formation in Pseudomonas aeruginosa.,Mawla GD, Hall BM, Carcamo-Oyarce G, Grant RA, Zhang JJ, Kardon JR, Ribbeck K, Sauer RT, Baker TA Mol Microbiol. 2020 Nov 24. doi: 10.1111/mmi.14649. PMID:33231899<ref>PMID:33231899</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7m1l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Clp protease 3D structures\|Clp protease 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Endopeptidase Clp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baker TA]]	+	[[Category: Baker, T A]]
-	[[Category: Grant RA]]	+	[[Category: Grant, R A]]
-	[[Category: Hall BM]]	+	[[Category: Hall, B M]]
-	[[Category: Sauer RT]]	+	[[Category: Sauer, R T]]
		+	[[Category: Hydrolase]]
		+	[[Category: Petidase]]

OCA: Protected "7m1l" [edit=sysop:move=sysop]

2021-03-24T06:42:55Z

Protected "7m1l" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:42, 24 March 2021

OCA: New page: ==Crystal structure of Pseudomonas aeruginosa ClpP2== == Structural highlights ==

2021-03-24T06:42:54Z

New page: ==Crystal structure of Pseudomonas aeruginosa ClpP2== <StructureSection load='7m1l' size='340' side='right'caption='7m1l' scene=''> == Structural highlights == <table><tr><td colspan=...

New page

==Crystal structure of Pseudomonas aeruginosa ClpP2==
<StructureSection load='7m1l' size='340' side='right'caption='[[7m1l]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M1L FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m1l OCA], [https://pdbe.org/7m1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m1l RCSB], [https://www.ebi.ac.uk/pdbsum/7m1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m1l ProSAT]</span></td></tr>
</table>
TOC
</StructureSection>
[[Category: Large Structures]]
[[Category: Baker TA]]
[[Category: Grant RA]]
[[Category: Hall BM]]
[[Category: Sauer RT]]