7ml9 - Revision history

OCA at 10:39, 22 May 2024

2024-05-22T10:39:11Z

←Older revision		Revision as of 10:39, 22 May 2024
Line 3:		Line 3:
	<StructureSection load='7ml9' size='340' side='right'caption='[[7ml9]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='7ml9' size='340' side='right'caption='[[7ml9]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ml9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_4517 Atcc 4517~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ML9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ML9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ml9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacillus_laterosporus Brevibacillus laterosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ML9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ML9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ml9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ml9 OCA], [https://pdbe.org/7ml9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ml9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ml9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ml9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ml9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ml9 OCA], [https://pdbe.org/7ml9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ml9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ml9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ml9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A249JQG5_BRELA A0A249JQG5_BRELA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 4517~~]]	+	[[Category: Brevibacillus laterosporus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Evdokimov, A]]	+	[[Category: Evdokimov A]]
-	[[Category: Rydel~~, T J~~]]	+	[[Category: Rydel TJ]]
-	[[Category: Zheng, M]]	+	[[Category: Zheng M]]
-	~~[[Category: Beta-pore-forming protein]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 15:51, 27 October 2021

2021-10-27T15:51:47Z

←Older revision		Revision as of 15:51, 27 October 2021
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ml9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ml9 OCA], [https://pdbe.org/7ml9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ml9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ml9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ml9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ml9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ml9 OCA], [https://pdbe.org/7ml9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ml9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ml9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ml9 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is a major corn pest of significant economic importance in the United States. The continuous need to control this corn maize pest and the development of field-evolved resistance toward all existing transgenic maize (Zea mays L.) expressing Bacillus thuringiensis (Bt) insecticidal proteins against WCR has prompted the development of new insect-protected crops expressing distinct structural classes of insecticidal proteins. In this current study, we describe the crystal structure and functional characterization of Mpp75Aa1.1, which represents the first corn rootworm (CRW) active insecticidal protein member of the ETX_MTX2 sub-family of beta-pore forming proteins (beta-PFPs), and provides new and effective protection against WCR feeding. The Mpp75Aa1.1 crystal structure was solved at 1.94 A resolution. The Mpp75Aa1.1 is processed at its carboxyl-terminus by WCR midgut proteases, forms an oligomer, and specifically interacts with putative membrane-associated binding partners on the midgut apical microvilli to cause cellular tissue damage resulting in insect death. Alanine substitution of the surface-exposed amino acids W206, Y212, and G217 within the Mpp75Aa1.1 putative receptor binding domain I demonstrates that at least these three amino acids are required for WCR activity. The distinctive spatial arrangement of these amino acids suggests that they are part of a receptor binding epitope, which may be unique to Mpp75Aa1.1 and not present in other ETX_MTX2 proteins that do not have WCR activity. Overall, this work establishes that Mpp75Aa1.1 shares a mode of action consistent with traditional WCR-active Bt proteins despite significant structural differences.
		+
		+	Structural and functional characterization of Mpp75Aa1.1, a putative beta-pore forming protein from Brevibacillus laterosporus active against the western corn rootworm.,Kouadio JL, Duff S, Aikins M, Zheng M, Rydel T, Chen D, Bretsnyder E, Xia C, Zhang J, Milligan J, Evdokimov A, Nageotte J, Yin Y, Moar W, Giddings K, Park Y, Jerga A, Haas J PLoS One. 2021 Oct 11;16(10):e0258052. doi: 10.1371/journal.pone.0258052., eCollection 2021. PMID:34634061<ref>PMID:34634061</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ml9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:52, 2 June 2021

2021-06-02T14:52:54Z

←Older revision		Revision as of 14:52, 2 June 2021
Line 1:		Line 1:

	==The Mpp75Aa1.1 beta-pore-forming protein from Brevibacillus laterosporus==		==The Mpp75Aa1.1 beta-pore-forming protein from Brevibacillus laterosporus==
-	<StructureSection load='7ml9' size='340' side='right'caption='[[7ml9]]' scene=''>	+	<StructureSection load='7ml9' size='340' side='right'caption='[[7ml9]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ML9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ML9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ml9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_4517 Atcc 4517]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ML9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ML9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ml9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ml9 OCA], [https://pdbe.org/7ml9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ml9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ml9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ml9 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ml9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ml9 OCA], [https://pdbe.org/7ml9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ml9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ml9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ml9 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 4517]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Evdokimov A]]	+	[[Category: Evdokimov, A]]
-	[[Category: Rydel TJ]]	+	[[Category: Rydel, T J]]
-	[[Category: Zheng M]]	+	[[Category: Zheng, M]]
		+	[[Category: Beta-pore-forming protein]]
		+	[[Category: Toxin]]

OCA: Protected "7ml9" [edit=sysop:move=sysop]

2021-05-05T08:54:48Z

Protected "7ml9" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:54, 5 May 2021

OCA: New page: ==The Mpp75Aa1.1 beta-pore-forming protein from Brevibacillus laterosporus== == Structural highlights == ...

2021-05-05T08:54:47Z

New page: ==The Mpp75Aa1.1 beta-pore-forming protein from Brevibacillus laterosporus== <StructureSection load='7ml9' size='340' side='right'caption='7ml9' scene=''> == Structural highlights == ...

New page

==The Mpp75Aa1.1 beta-pore-forming protein from Brevibacillus laterosporus==
<StructureSection load='7ml9' size='340' side='right'caption='[[7ml9]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ML9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ML9 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ml9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ml9 OCA], [https://pdbe.org/7ml9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ml9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ml9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ml9 ProSAT]</span></td></tr>
</table>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Evdokimov A]]
[[Category: Rydel TJ]]
[[Category: Zheng M]]