7mld - Revision history

OCA at 16:14, 18 October 2023

2023-10-18T16:14:49Z

←Older revision		Revision as of 16:14, 18 October 2023
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	==PYL10 bound to the ABA pan-antagonist antabactin==		==PYL10 bound to the ABA pan-antagonist antabactin==
-	<StructureSection load='7mld' size='340' side='right'caption='[[7mld]]' scene=''>	+	<StructureSection load='7mld' size='340' side='right'caption='[[7mld]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MLD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MLD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mld]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MLD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MLD FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mld OCA], [https://pdbe.org/7mld PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mld RCSB], [https://www.ebi.ac.uk/pdbsum/7mld PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mld ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=ZLG:antabactin'>ZLG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mld OCA], [https://pdbe.org/7mld PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mld RCSB], [https://www.ebi.ac.uk/pdbsum/7mld PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mld ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PYL10_ARATH PYL10_ARATH] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Abscisic acid (ABA) is a key plant hormone that mediates both plant biotic and abiotic stress responses and many other developmental processes. ABA receptor antagonists are useful for dissecting and manipulating ABA's physiological roles in vivo. We set out to design antagonists that block receptor-PP2C interactions by modifying the agonist opabactin (OP), a synthetically accessible, high-affinity scaffold. Click chemistry was used to create an approximately 4,000-member library of C4-diversified opabactin derivatives that were screened for receptor antagonism in vitro. This revealed a peptidotriazole motif shared among hits, which we optimized to yield antabactin (ANT), a pan-receptor antagonist. An X-ray crystal structure of an ANT-PYL10 complex (1.86 A) reveals that ANT's peptidotriazole headgroup is positioned to sterically block receptor-PP2C interactions in the 4' tunnel and stabilizes a noncanonical closed-gate receptor conformer that partially opens to accommodate ANT binding. To facilitate binding-affinity studies using fluorescence polarization, we synthesized TAMRA-ANT. Equilibrium dissociation constants for TAMRA-ANT binding to Arabidopsis receptors range from approximately 400 to 1,700 pM. ANT displays improved activity in vivo and disrupts ABA-mediated processes in multiple species. ANT is able to accelerate seed germination in Arabidopsis, tomato, and barley, suggesting that it could be useful as a germination stimulant in species where endogenous ABA signaling limits seed germination. Thus, click-based diversification of a synthetic agonist scaffold allowed us to rapidly develop a high-affinity probe of ABA-receptor function for dissecting and manipulating ABA signaling.
		+
		+	Click-to-lead design of a picomolar ABA receptor antagonist with potent activity in vivo.,Vaidya AS, Peterson FC, Eckhardt J, Xing Z, Park SY, Dejonghe W, Takeuchi J, Pri-Tal O, Faria J, Elzinga D, Volkman BF, Todoroki Y, Mosquna A, Okamoto M, Cutler SR Proc Natl Acad Sci U S A. 2021 Sep 21;118(38). pii: 2108281118. doi:, 10.1073/pnas.2108281118. PMID:34531324<ref>PMID:34531324</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mld" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Abscisic acid receptor 3D structures\|Abscisic acid receptor 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cutler SR]]		[[Category: Cutler SR]]

OCA at 08:50, 29 September 2021

2021-09-29T08:50:39Z

←Older revision		Revision as of 08:50, 29 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7mld ~~is ON HOLD until Paper Publication~~	+	==PYL10 bound to the ABA pan-antagonist antabactin==
-		+	<StructureSection load='7mld' size='340' side='right'caption='[[7mld]]' scene=''>
-	~~Authors~~: ~~Peterson~~, F.C., ~~Vaidya~~, A.S., ~~Volkman, B~~.F., ~~Cutler, S~~.R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MLD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MLD FirstGlance]. <br>
-	~~Description: PYL10 bound to the ABA pan-antagonist antabactin~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mld OCA], [https://pdbe.org/7mld PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mld RCSB], [https://www.ebi.ac.uk/pdbsum/7mld PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mld ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cutler~~, S.R~~]]	+	__TOC__
-	[[Category: Peterson~~, F.C~~]]	+	</StructureSection>
-	[[Category: ~~Volkman, B.F~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Vaidya, A.S~~]]	+	[[Category: Cutler SR]]
		+	[[Category: Peterson FC]]
		+	[[Category: Vaidya AS]]
		+	[[Category: Volkman BF]]

OCA at 11:04, 9 June 2021

2021-06-09T11:04:24Z

←Older revision		Revision as of 11:04, 9 June 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mld is ON HOLD	+	The entry 7mld is ON HOLD until Paper Publication

	Authors: Peterson, F.C., Vaidya, A.S., Volkman, B.F., Cutler, S.R.		Authors: Peterson, F.C., Vaidya, A.S., Volkman, B.F., Cutler, S.R.

OCA: Protected "7mld" [edit=sysop:move=sysop]

2021-05-05T07:27:52Z

Protected "7mld" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:27, 5 May 2021

OCA: New page: '''Unreleased structure''' The entry 7mld is ON HOLD Authors: Peterson, F.C., Vaidya, A.S., Volkman, B.F., Cutler, S.R. Description: PYL10 bound to the ABA pan-antagonist antabactin [[...

2021-05-05T07:27:51Z

New page: '''Unreleased structure''' The entry 7mld is ON HOLD Authors: Peterson, F.C., Vaidya, A.S., Volkman, B.F., Cutler, S.R. Description: PYL10 bound to the ABA pan-antagonist antabactin [[...

New page

'''Unreleased structure'''

The entry 7mld is ON HOLD

Authors: Peterson, F.C., Vaidya, A.S., Volkman, B.F., Cutler, S.R.

Description: PYL10 bound to the ABA pan-antagonist antabactin
[[Category: Unreleased Structures]]
[[Category: Cutler, S.R]]
[[Category: Peterson, F.C]]
[[Category: Volkman, B.F]]
[[Category: Vaidya, A.S]]