OCA at 19:40, 29 May 2024

2024-05-29T19:40:54Z

←Older revision		Revision as of 19:40, 29 May 2024
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-	====	+	==Mtb 70SIC in complex with MtbEttA at Trans_R1 state==
-	<StructureSection load='7msz' size='340' side='right'caption='[[7msz]]' scene=''>	+	<StructureSection load='7msz' size='340' side='right'caption='[[7msz]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7msz]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MSZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7msz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7msz OCA], [https://pdbe.org/7msz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7msz RCSB], [https://www.ebi.ac.uk/pdbsum/7msz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7msz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=4OC:4N,O2-METHYLCYTIDINE-5-MONOPHOSPHATE'>4OC</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=G7M:N7-METHYL-GUANOSINE-5-MONOPHOSPHATE'>G7M</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7msz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7msz OCA], [https://pdbe.org/7msz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7msz RCSB], [https://www.ebi.ac.uk/pdbsum/7msz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7msz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RL32_MYCTU RL32_MYCTU]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	EttA, energy-dependent translational throttle A, is a ribosomal factor that gates ribosome entry into the translation elongation cycle. A detailed understanding of its mechanism of action is limited due to the lack of high-resolution structures along its ATPase cycle. Here we present the cryo-electron microscopy (cryo-EM) structures of EttA from Mycobacterium tuberculosis (Mtb), referred to as MtbEttA, in complex with the Mtb 70S ribosome initiation complex (70SIC) at the pre-hydrolysis (ADPNP) and transition (ADP-VO4) states, and the crystal structure of MtbEttA alone in the post-hydrolysis (ADP) state. We observe that MtbEttA binds the E-site of the Mtb 70SIC, remodeling the P-site tRNA and the ribosomal intersubunit bridge B7a during the ribosomal ratcheting. In return, the rotation of the 30S causes conformational changes in MtbEttA, forcing the two nucleotide-binding sites (NBSs) to alternate to engage each ADPNP in the pre-hydrolysis states, followed by complete engagements of both ADP-VO4 molecules in the ATP-hydrolysis transition states. In the post-hydrolysis state, the conserved ATP-hydrolysis motifs of MtbEttA dissociate from both ADP molecules, leaving two nucleotide-binding domains (NBDs) in an open conformation. These structures reveal a dynamic interplay between MtbEttA and the Mtb ribosome, providing insights into the mechanism of translational regulation by EttA-like proteins.
		+
		+	Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis.,Cui Z, Li X, Shin J, Gamper H, Hou YM, Sacchettini JC, Zhang J Nat Commun. 2022 Jan 21;13(1):432. doi: 10.1038/s41467-022-28078-1. PMID:35064151<ref>PMID:35064151</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7msz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribosome 3D structures\|Ribosome 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
		+	[[Category: Cui Z]]
		+	[[Category: Zhang J]]

OCA at 07:00, 2 February 2022

2022-02-02T07:00:09Z

←Older revision		Revision as of 07:00, 2 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7msz ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7msz' size='340' side='right'caption='[[7msz]]' scene=''>
-	~~Authors~~: ~~Cui, Z~~.~~, Zhang, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Mtb 70SIC in complex with MtbEttA at Trans_R1 state~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7msz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7msz OCA], [https://pdbe.org/7msz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7msz RCSB], [https://www.ebi.ac.uk/pdbsum/7msz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7msz ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Cui, Z~~]]	+	__TOC__
-	[[Category: ~~Zhang, J~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 13:37, 17 June 2021

2021-06-17T13:37:38Z

←Older revision		Revision as of 13:37, 17 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7msz is ON HOLD	+	The entry 7msz is ON HOLD until Paper Publication

	Authors: Cui, Z., Zhang, J.		Authors: Cui, Z., Zhang, J.

OCA: Protected "7msz" [edit=sysop:move=sysop]

2021-05-19T08:58:24Z

Protected "7msz" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:58, 19 May 2021

OCA: New page: '''Unreleased structure''' The entry 7msz is ON HOLD Authors: Cui, Z., Zhang, J. Description: Mtb 70SIC in complex with MtbEttA at Trans_R1 state Category: Unreleased Structures [[...

2021-05-19T08:58:22Z

New page: '''Unreleased structure''' The entry 7msz is ON HOLD Authors: Cui, Z., Zhang, J. Description: Mtb 70SIC in complex with MtbEttA at Trans_R1 state Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 7msz is ON HOLD

Authors: Cui, Z., Zhang, J.

Description: Mtb 70SIC in complex with MtbEttA at Trans_R1 state
[[Category: Unreleased Structures]]
[[Category: Cui, Z]]
[[Category: Zhang, J]]

7msz - Revision history

OCA at 19:40, 29 May 2024

OCA at 07:00, 2 February 2022

OCA at 13:37, 17 June 2021

OCA: Protected "7msz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7msz is ON HOLD Authors: Cui, Z., Zhang, J. Description: Mtb 70SIC in complex with MtbEttA at Trans_R1 state Category: Unreleased Structures [[...