OCA at 12:55, 13 October 2021

2021-10-13T12:55:24Z

←Older revision		Revision as of 12:55, 13 October 2021
Line 1:		Line 1:

	==Cryo-EM structure of minimal TRPV1 with 2 bound RTX in opposite pockets==		==Cryo-EM structure of minimal TRPV1 with 2 bound RTX in opposite pockets==
-	<StructureSection load='7mze' size='340' side='right'caption='[[7mze]]' scene=''>	+	<StructureSection load='7mze' size='340' side='right'caption='[[7mze]], [[Resolution\|resolution]] 3.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mze]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mze OCA], [https://pdbe.org/7mze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mze RCSB], [https://www.ebi.ac.uk/pdbsum/7mze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mze ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6EU:RESINIFERATOXIN'>6EU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=XJ7:(2S)-1-(butanoyloxy)-3-{[(R)-hydroxy{[(1r,2R,3S,4S,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propan-2-yl+tridecanoate'>XJ7</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mze OCA], [https://pdbe.org/7mze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mze RCSB], [https://www.ebi.ac.uk/pdbsum/7mze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mze ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/TRPV1_RAT TRPV1_RAT]] Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.<ref>PMID:9349813</ref> <ref>PMID:10644739</ref> <ref>PMID:11140687</ref> <ref>PMID:11418861</ref> <ref>PMID:12095983</ref> <ref>PMID:12194871</ref> <ref>PMID:12808128</ref> <ref>PMID:14523239</ref> <ref>PMID:12764195</ref> <ref>PMID:14630912</ref> <ref>PMID:15173182</ref> <ref>PMID:21076423</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many transient receptor potential (TRP) channels respond to diverse stimuli and conditionally conduct small and large cations. Such functional plasticity is presumably enabled by a uniquely dynamic ion selectivity filter that is regulated by physiological agents. What is currently missing is a "photo series" of intermediate structural states that directly address this hypothesis and reveal specific mechanisms behind such dynamic channel regulation. Here, we exploit cryoelectron microscopy (cryo-EM) to visualize conformational transitions of the capsaicin receptor, TRPV1, as a model to understand how dynamic transitions of the selectivity filter in response to algogenic agents, including protons, vanilloid agonists, and peptide toxins, permit permeation by small and large organic cations. These structures also reveal mechanisms governing ligand binding substates, as well as allosteric coupling between key sites that are proximal to the selectivity filter and cytoplasmic gate. These insights suggest a general framework for understanding how TRP channels function as polymodal signal integrators.
		+
		+	Structural snapshots of TRPV1 reveal mechanism of polymodal functionality.,Zhang K, Julius D, Cheng Y Cell. 2021 Aug 31. pii: S0092-8674(21)00982-X. doi: 10.1016/j.cell.2021.08.012. PMID:34496225<ref>PMID:34496225</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mze" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cheng Y]]	+	[[Category: Cheng, Y]]
-	[[Category: Julius D]]	+	[[Category: Julius, D]]
-	[[Category: Zhang K]]	+	[[Category: Zhang, K]]
		+	[[Category: Cryo-em]]
		+	[[Category: Nanodisc]]
		+	[[Category: Stoichiometry]]
		+	[[Category: Transport protein]]
		+	[[Category: Trp channel]]

OCA at 06:52, 22 September 2021

2021-09-22T06:52:23Z

←Older revision		Revision as of 06:52, 22 September 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7mze is ON HOLD until Paper Publication~~	+	==Cryo-EM structure of minimal TRPV1 with 2 bound RTX in opposite pockets==
-		+	<StructureSection load='7mze' size='340' side='right'caption='[[7mze]]' scene=''>
-	~~Authors: Zhang, K., Julius, D., Cheng, Y.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZE FirstGlance]. <br>
-	~~Description:~~ Cryo-EM structure of minimal TRPV1 with 2 bound RTX in opposite pockets	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mze OCA], [https://pdbe.org/7mze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mze RCSB], [https://www.ebi.ac.uk/pdbsum/7mze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mze ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cheng, Y]]	+	__TOC__
-	[[Category: ~~Zhang, K~~]]	+	</StructureSection>
-	[[Category: ~~Julius, D~~]]	+	[[Category: Large Structures]]
		+	[[Category: Cheng Y]]
		+	[[Category: Julius D]]
		+	[[Category: Zhang K]]

OCA at 02:27, 2 July 2021

2021-07-02T02:27:43Z

←Older revision		Revision as of 02:27, 2 July 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mze is ON HOLD	+	The entry 7mze is ON HOLD until Paper Publication

	Authors: Zhang, K., Julius, D., Cheng, Y.		Authors: Zhang, K., Julius, D., Cheng, Y.

OCA: Protected "7mze" [edit=sysop:move=sysop]

2021-06-02T13:30:56Z

Protected "7mze" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:30, 2 June 2021

OCA: New page: '''Unreleased structure''' The entry 7mze is ON HOLD Authors: Zhang, K., Julius, D., Cheng, Y. Description: Cryo-EM structure of minimal TRPV1 with 2 bound RTX in opposite pockets [[Ca...

2021-06-02T13:30:55Z

New page: '''Unreleased structure''' The entry 7mze is ON HOLD Authors: Zhang, K., Julius, D., Cheng, Y. Description: Cryo-EM structure of minimal TRPV1 with 2 bound RTX in opposite pockets [[Ca...

New page

'''Unreleased structure'''

The entry 7mze is ON HOLD

Authors: Zhang, K., Julius, D., Cheng, Y.

Description: Cryo-EM structure of minimal TRPV1 with 2 bound RTX in opposite pockets
[[Category: Unreleased Structures]]
[[Category: Cheng, Y]]
[[Category: Zhang, K]]
[[Category: Julius, D]]

7mze - Revision history

OCA at 12:55, 13 October 2021

OCA at 06:52, 22 September 2021

OCA at 02:27, 2 July 2021

OCA: Protected "7mze" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7mze is ON HOLD Authors: Zhang, K., Julius, D., Cheng, Y. Description: Cryo-EM structure of minimal TRPV1 with 2 bound RTX in opposite pockets [[Ca...