OCA at 20:22, 16 November 2022

2022-11-16T20:22:06Z

←Older revision		Revision as of 20:22, 16 November 2022
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-	====	+	==Cryo-EM structure of broadly neutralizing antibody 2-36 in complex with prefusion SARS-CoV-2 spike glycoprotein==
-	<StructureSection load='7n5h' size='340' side='right'caption='[[7n5h]]' scene=''>	+	<StructureSection load='7n5h' size='340' side='right'caption='[[7n5h]], [[Resolution\|resolution]] 3.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7n5h]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N5H FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5h OCA], [https://pdbe.org/7n5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5h RCSB], [https://www.ebi.ac.uk/pdbsum/7n5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5h ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5h OCA], [https://pdbe.org/7n5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5h RCSB], [https://www.ebi.ac.uk/pdbsum/7n5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5h ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The repeated emergence of highly pathogenic human coronaviruses as well as their evolving variants highlight the need to develop potent and broad-spectrum antiviral therapeutics and vaccines. By screening monoclonal antibodies (mAbs) isolated from COVID-19-convalescent patients, we found one mAb, 2-36, with cross-neutralizing activity against SARS-CoV. We solved the cryo-EM structure of 2-36 in complex with SARS-CoV-2 or SARS-CoV spike, revealing a highly conserved epitope in the receptor-binding domain (RBD). Antibody 2-36 neutralized not only all current circulating SARS-CoV-2 variants and SARS-COV, but also a panel of bat and pangolin sarbecoviruses that can use human angiotensin-converting enzyme 2 (ACE2) as a receptor. We selected 2-36-escape viruses in vitro and confirmed that K378 T in SARS-CoV-2 RBD led to viral resistance. Taken together, 2-36 represents a strategic reserve drug candidate for the prevention and treatment of possible diseases caused by pre-emergent SARS-related coronaviruses. Its epitope defines a promising target for the development of a pan-sarbecovirus vaccine.
		+
		+	A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses.,Wang P, Casner RG, Nair MS, Yu J, Guo Y, Wang M, Chan JF, Cerutti G, Iketani S, Liu L, Sheng Z, Chen Z, Yuen KY, Kwong PD, Huang Y, Shapiro L, Ho DD Emerg Microbes Infect. 2022 Dec;11(1):147-157. doi:, 10.1080/22221751.2021.2011623. PMID:34836485<ref>PMID:34836485</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7n5h" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Casner RG]]
		+	[[Category: Cerutti G]]
		+	[[Category: Shapiro L]]

OCA at 14:30, 3 November 2021

2021-11-03T14:30:57Z

←Older revision		Revision as of 14:30, 3 November 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7n5h ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7n5h' size='340' side='right'caption='[[7n5h]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5h OCA], [https://pdbe.org/7n5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5h RCSB], [https://www.ebi.ac.uk/pdbsum/7n5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5h ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:46, 7 July 2021

2021-07-07T08:46:51Z

←Older revision		Revision as of 08:46, 7 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7n5h is ON HOLD	+	The entry 7n5h is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7n5h" [edit=sysop:move=sysop]

2021-06-17T13:39:33Z

Protected "7n5h" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:39, 17 June 2021

OCA: New page: '''Unreleased structure''' The entry 7n5h is ON HOLD Authors: Description: Category: Unreleased Structures

2021-06-17T13:39:32Z

New page: '''Unreleased structure''' The entry 7n5h is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7n5h is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7n5h - Revision history

OCA at 20:22, 16 November 2022

OCA at 14:30, 3 November 2021

OCA at 08:46, 7 July 2021

OCA: Protected "7n5h" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7n5h is ON HOLD Authors: Description: Category: Unreleased Structures