7na6 - Revision history

OCA at 12:56, 13 October 2021

2021-10-13T12:56:13Z

←Older revision		Revision as of 12:56, 13 October 2021
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	==Cryo-EM structure of AAV True Type==		==Cryo-EM structure of AAV True Type==
-	<StructureSection load='7na6' size='340' side='right'caption='[[7na6]]' scene=''>	+	<StructureSection load='7na6' size='340' side='right'caption='[[7na6]], [[Resolution\|resolution]] 3.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NA6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7na6]] is a 60 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NA6 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7na6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7na6 OCA], [https://pdbe.org/7na6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7na6 RCSB], [https://www.ebi.ac.uk/pdbsum/7na6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7na6 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7na6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7na6 OCA], [https://pdbe.org/7na6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7na6 RCSB], [https://www.ebi.ac.uk/pdbsum/7na6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7na6 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Adeno-associated viruses (AAV) are utilized as gene transfer vectors in the treatment of monogenic disorders. A variant, rationally engineered based on natural AAV2 isolates, designated AAV-True Type (AAV-TT), is highly neurotropic compared to wild type AAV2 in vivo, and vectors based on it, are currently being evaluated for central nervous system applications. AAV-TT differs from AAV2 by 14 amino acids, including R585S and R588T, two residues previously shown to be essential for heparan sulfate binding of AAV2. The capsid structures of AAV-TT and AAV2 visualized by cryo-electron microscopy at 3.4 and 3.0 A resolution, respectively, highlighted structural perturbations at specific amino acid differences. Differential scanning fluorimetry (DSF) performed at different pH conditions demonstrated that the melting temperature (Tm) of AAV2 was consistently approximately 5 degrees C lower than AAV-TT, but both showed maximal stability at pH 5.5, corresponding to the pH in the late endosome, proposed as required for VP1u externalization to facilitate endosomal escape. Reintroduction of arginines at positions 585 and 588 in AAV-TT caused a reduction in Tm, demonstrating that the lack of basic amino acids at these positions are associated with capsid stability. These results provide structural and thermal annotation of AAV2/AAV-TT residue differences, that account for divergent cell binding, transduction, antigenic reactivity, and transduction of permissive tissues between the two viruses. Specifically, these data indicate that AAV-TT may not utilize a glycan receptor mediated pathway to enter cells and may have lower antigenic properties as compared to AAV2.
		+
		+	Comparative structural, biophysical, and receptor binding study of true type and wild type AAV2.,Bennett A, Hull J, Jolinon N, Tordo J, Moss K, Binns E, Mietzsch M, Hagemann C, Linden RM, Serio A, Chipman P, Sousa D, Broecker F, Seeberger P, Henckaerts E, McKenna R, Agbandje-McKenna M J Struct Biol. 2021 Sep 10;213(4):107795. doi: 10.1016/j.jsb.2021.107795. PMID:34509611<ref>PMID:34509611</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7na6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bennett AD]]	+	[[Category: Bennett, A D]]
-	[[Category: McKenna R]]	+	[[Category: McKenna, R]]
		+	[[Category: Beta-barrel]]
		+	[[Category: Icosahedron]]
		+	[[Category: Therapeutic]]
		+	[[Category: Vector]]
		+	[[Category: Virus]]

OCA at 08:50, 29 September 2021

2021-09-29T08:50:55Z

←Older revision		Revision as of 08:50, 29 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7na6 ~~is ON HOLD until Paper Publication~~	+	==Cryo-EM structure of AAV True Type==
-		+	<StructureSection load='7na6' size='340' side='right'caption='[[7na6]]' scene=''>
-	~~Authors~~: ~~Bennett~~, A.D., ~~McKenna~~, R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NA6 FirstGlance]. <br>
-	~~Description~~: ~~Cryo~~-~~EM structure of AAV True Type~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7na6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7na6 OCA], [https://pdbe.org/7na6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7na6 RCSB], [https://www.ebi.ac.uk/pdbsum/7na6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7na6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Bennett~~, A.D~~]]	+	__TOC__
-	[[Category: ~~Mckenna,~~ R]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bennett AD]]
		+	[[Category: McKenna R]]

OCA at 07:51, 21 July 2021

2021-07-21T07:51:55Z

←Older revision		Revision as of 07:51, 21 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7na6 is ON HOLD	+	The entry 7na6 is ON HOLD until Paper Publication

	Authors: Bennett, A.D., McKenna, R.		Authors: Bennett, A.D., McKenna, R.

OCA: Protected "7na6" [edit=sysop:move=sysop]

2021-07-02T02:30:40Z

Protected "7na6" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:30, 2 July 2021

OCA: New page: '''Unreleased structure''' The entry 7na6 is ON HOLD Authors: Bennett, A.D., McKenna, R. Description: Cryo-EM structure of AAV True Type Category: Unreleased Structures [[Category:...

2021-07-02T02:30:39Z

New page: '''Unreleased structure''' The entry 7na6 is ON HOLD Authors: Bennett, A.D., McKenna, R. Description: Cryo-EM structure of AAV True Type Category: Unreleased Structures [[Category:...

New page

'''Unreleased structure'''

The entry 7na6 is ON HOLD

Authors: Bennett, A.D., McKenna, R.

Description: Cryo-EM structure of AAV True Type
[[Category: Unreleased Structures]]
[[Category: Bennett, A.D]]
[[Category: Mckenna, R]]