7nqf - Revision history

OCA at 12:38, 1 February 2024

2024-02-01T12:38:20Z

←Older revision		Revision as of 12:38, 1 February 2024
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-	====	+	==Prim-Pol Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137 with dsDNA==
-	<StructureSection load='7nqf' size='340' side='right'caption='[[7nqf]]' scene=''>	+	<StructureSection load='7nqf' size='340' side='right'caption='[[7nqf]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nqf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinitoga_sp._1137 Marinitoga sp. 1137] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NQF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nqf OCA], [https://pdbe.org/7nqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nqf RCSB], [https://www.ebi.ac.uk/pdbsum/7nqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nqf ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.02Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nqf OCA], [https://pdbe.org/7nqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nqf RCSB], [https://www.ebi.ac.uk/pdbsum/7nqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nqf ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/H2J4R1_MARPK H2J4R1_MARPK]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During the initiation of DNA replication, oligonucleotide primers are synthesized de novo by primases and are subsequently extended by replicative polymerases to complete genome duplication. The primase-polymerase (Prim-Pol) superfamily is a diverse grouping of primases, which includes replicative primases and CRISPR-associated primase-polymerases (CAPPs) involved in adaptive immunity(1-3). Although much is known about the activities of these enzymes, the precise mechanism used by primases to initiate primer synthesis has not been elucidated. Here we identify the molecular bases for the initiation of primer synthesis by CAPP and show that this mechanism is also conserved in replicative primases. The crystal structure of a primer initiation complex reveals how the incoming nucleotides are positioned within the active site, adjacent to metal cofactors and paired to the templating single-stranded DNA strand, before synthesis of the first phosphodiester bond. Furthermore, the structure of a Prim-Pol complex with double-stranded DNA shows how the enzyme subsequently extends primers in a processive polymerase mode. The structural and mechanistic studies presented here establish how Prim-Pol proteins instigate primer synthesis, revealing the requisite molecular determinants for primer synthesis within the catalytic domain. This work also establishes that the catalytic domain of Prim-Pol enzymes, including replicative primases, is sufficient to catalyse primer formation.
		+
		+	Molecular basis for the initiation of DNA primer synthesis.,Li AWH, Zabrady K, Bainbridge LJ, Zabrady M, Naseem-Khan S, Berger MB, Kolesar P, Cisneros GA, Doherty AJ Nature. 2022 May 4. pii: 10.1038/s41586-022-04695-0. doi:, 10.1038/s41586-022-04695-0. PMID:35508653<ref>PMID:35508653</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nqf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Marinitoga sp. 1137]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Doherty AJ]]
		+	[[Category: Li AWH]]

OCA at 12:47, 4 May 2022

2022-05-04T12:47:22Z

←Older revision		Revision as of 12:47, 4 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7nqf ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7nqf' size='340' side='right'caption='[[7nqf]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nqf OCA], [https://pdbe.org/7nqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nqf RCSB], [https://www.ebi.ac.uk/pdbsum/7nqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nqf ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:29, 31 March 2021

2021-03-31T09:29:59Z

←Older revision		Revision as of 09:29, 31 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nqf is ON HOLD	+	The entry 7nqf is ON HOLD until Paper Publication

-	Authors: ~~Li, A.W.H., Doherty, A.J.~~	+	Authors:

-	Description: ~~Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137 with dGTP~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Doherty, A.J]]
-	[[Category: Li, A.W.H]]

OCA: Protected "7nqf" [edit=sysop:move=sysop]

2021-03-10T15:03:58Z

Protected "7nqf" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:03, 10 March 2021

OCA: New page: '''Unreleased structure''' The entry 7nqf is ON HOLD Authors: Li, A.W.H., Doherty, A.J. Description: Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from M...

2021-03-10T15:03:56Z

New page: '''Unreleased structure''' The entry 7nqf is ON HOLD Authors: Li, A.W.H., Doherty, A.J. Description: Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from M...

New page

'''Unreleased structure'''

The entry 7nqf is ON HOLD

Authors: Li, A.W.H., Doherty, A.J.

Description: Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137 with dGTP
[[Category: Unreleased Structures]]
[[Category: Doherty, A.J]]
[[Category: Li, A.W.H]]